Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453 |
Resumo: | Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents. The massive use of antibiotics is among the leading causes of bacterial resistance. The objective of this study is to investigate whether the sewage of the Maringá Regional University Hospital (HUM) has pathogenic bacteria resistant to antibiotics. The samples were collected at two sites, one from the entire hospital and the other from the blood bank. 100 mL were inserted in dilutions of 10-2, 10-3 and 10-4 in cultures Mac Conkey Agar, Salmonella-Shigella Agar, Mannitol Salt Agar and Sabouraud Dextrose Agar. The samples were subjected to bacterial identification and antibiogram. The identification was based on bacterioscopy by Gram staining, and biochemical evidence. The antibiograms were made in accordance with the disk diffusion methodology in Müeller-Hinton agar, and 12 antibiotics were used in these tests. They were isolated and 39 samples were identified; of these, 18 species showed resistance and moderate resistance to at least one antibiotic. Bacteria isolated and identified as K. pneumoniae, E. coli, K. pneumoniae, E. cloacae and C. freundii showed resistance to almost all tested antibiotics. It was found that the HUM releases bacteria with multi-resistant to certain antibiotics into the public sewage system. Therefore, it becomes to deploy an efficient system of treating effluents in the HUM. |
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Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453Avaliação da resistência a antibióticos de bactérias isoladas de efluente hospitalar - DOI: 10.4025/actascitechnol.v32i1.7453bacterial resistancehospital effluentantibioticsresistência bacterianaefluente hospitalarantibióticosEvaluation of resistance to antibiotics of bacteria isolated from hospital effluents. The massive use of antibiotics is among the leading causes of bacterial resistance. The objective of this study is to investigate whether the sewage of the Maringá Regional University Hospital (HUM) has pathogenic bacteria resistant to antibiotics. The samples were collected at two sites, one from the entire hospital and the other from the blood bank. 100 mL were inserted in dilutions of 10-2, 10-3 and 10-4 in cultures Mac Conkey Agar, Salmonella-Shigella Agar, Mannitol Salt Agar and Sabouraud Dextrose Agar. The samples were subjected to bacterial identification and antibiogram. The identification was based on bacterioscopy by Gram staining, and biochemical evidence. The antibiograms were made in accordance with the disk diffusion methodology in Müeller-Hinton agar, and 12 antibiotics were used in these tests. They were isolated and 39 samples were identified; of these, 18 species showed resistance and moderate resistance to at least one antibiotic. Bacteria isolated and identified as K. pneumoniae, E. coli, K. pneumoniae, E. cloacae and C. freundii showed resistance to almost all tested antibiotics. It was found that the HUM releases bacteria with multi-resistant to certain antibiotics into the public sewage system. Therefore, it becomes to deploy an efficient system of treating effluents in the HUM.O uso intensivo de antibióticos está entre as principais causas de resistência bacteriana. O objetivo deste estudo foi investigar se o esgoto do Hospital Universitário Regional de Maringá (HUM) apresentava bactérias patogênicas resistentes a antibióticos. As amostras foram coletadas em dois pontos, um de todo o hospital e outro do Hemocentro. Foram semeados 100 µL nas diluições 10-2, 10-3 e 10-4 nos meios de culturas Ágar Mac Conkey, Ágar Salmonella-Shigella, Ágar Manitol Salgado e Ágar Sabouraud Dextrose. As amostras foram submetidas à identificação bacteriana e ao antibiograma. A identificação bacteriana se baseou na bacterioscopia por coloração de Gram e provas bioquímicas. Os antibiogramas foram feitos de acordo com a metodologia de difusão de disco em Ágar Müeller-Hinton, e foram utilizados 12 antibióticos nesses testes. Foram isoladas e identificadas 39 amostras, e dentre estas, 18 espécies apresentaram resistência e moderada resistência pelo menos para um dos antibióticos. Bactérias isoladas e identificadas como K. pneumoniae, E.coli, K. pneumoniae, E. cloacae e C. freundii apresentaram resistência para quase todos os antibióticos testados. Verificou-se que o HUM lança, na rede pública coletora de esgoto, bactérias com multirresistência a determinados antibióticos. Portanto, torna-se necessária a implantação de sistema eficiente de tratamento de efluentes no HUM.Universidade Estadual De Maringá2009-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/745310.4025/actascitechnol.v32i1.7453Acta Scientiarum. Technology; Vol 32 No 1 (2010); 1-5Acta Scientiarum. Technology; v. 32 n. 1 (2010); 1-51806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453/7453Abreu, Elenice TavaresPretto, João AdelmoCaleare, Ângelo de OliveiraTavares, Célia Regina GranhenNakamura, Celso Vataruinfo:eu-repo/semantics/openAccess2024-05-17T13:03:09Zoai:periodicos.uem.br/ojs:article/7453Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:09Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 Avaliação da resistência a antibióticos de bactérias isoladas de efluente hospitalar - DOI: 10.4025/actascitechnol.v32i1.7453 |
title |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
spellingShingle |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 Abreu, Elenice Tavares bacterial resistance hospital effluent antibiotics resistência bacteriana efluente hospitalar antibióticos |
title_short |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
title_full |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
title_fullStr |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
title_full_unstemmed |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
title_sort |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents - DOI: 10.4025/actascitechnol.v32i1.7453 |
author |
Abreu, Elenice Tavares |
author_facet |
Abreu, Elenice Tavares Pretto, João Adelmo Caleare, Ângelo de Oliveira Tavares, Célia Regina Granhen Nakamura, Celso Vataru |
author_role |
author |
author2 |
Pretto, João Adelmo Caleare, Ângelo de Oliveira Tavares, Célia Regina Granhen Nakamura, Celso Vataru |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Abreu, Elenice Tavares Pretto, João Adelmo Caleare, Ângelo de Oliveira Tavares, Célia Regina Granhen Nakamura, Celso Vataru |
dc.subject.por.fl_str_mv |
bacterial resistance hospital effluent antibiotics resistência bacteriana efluente hospitalar antibióticos |
topic |
bacterial resistance hospital effluent antibiotics resistência bacteriana efluente hospitalar antibióticos |
description |
Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents. The massive use of antibiotics is among the leading causes of bacterial resistance. The objective of this study is to investigate whether the sewage of the Maringá Regional University Hospital (HUM) has pathogenic bacteria resistant to antibiotics. The samples were collected at two sites, one from the entire hospital and the other from the blood bank. 100 mL were inserted in dilutions of 10-2, 10-3 and 10-4 in cultures Mac Conkey Agar, Salmonella-Shigella Agar, Mannitol Salt Agar and Sabouraud Dextrose Agar. The samples were subjected to bacterial identification and antibiogram. The identification was based on bacterioscopy by Gram staining, and biochemical evidence. The antibiograms were made in accordance with the disk diffusion methodology in Müeller-Hinton agar, and 12 antibiotics were used in these tests. They were isolated and 39 samples were identified; of these, 18 species showed resistance and moderate resistance to at least one antibiotic. Bacteria isolated and identified as K. pneumoniae, E. coli, K. pneumoniae, E. cloacae and C. freundii showed resistance to almost all tested antibiotics. It was found that the HUM releases bacteria with multi-resistant to certain antibiotics into the public sewage system. Therefore, it becomes to deploy an efficient system of treating effluents in the HUM. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453 10.4025/actascitechnol.v32i1.7453 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453 |
identifier_str_mv |
10.4025/actascitechnol.v32i1.7453 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453/7453 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 32 No 1 (2010); 1-5 Acta Scientiarum. Technology; v. 32 n. 1 (2010); 1-5 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315333721358336 |