Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550 |
Resumo: | Lipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as inducer. The experiments were conduced in 250 mL Erlenmeyer flasks in a shaker (170 rpm), containing 125 mL (%, w v-1) of KH2PO4, MgSO4.7H2O, NaNO3, yeast extract, peptone and starch. The pH was 5.0 and 7.0; incubation temperature was 30 and 37°C, respectively; fermentation time was 24, 48, 72, 96h, 120 and 144h; and the inducer concentration was 3 and 4%, added after 72h of fermentation. The determination of lipolytic activity was examined at 37°C, in a shaker in thermostatic bath with rotation of 82 rpm, using gum arabic 7% (w v-1), olive oil and water. The reactions were conducted for 10 min with agitation and later stopped by the addition of an acetone:ethanol:water solution, where released fatty acids were titrated with a solution of KOH (0.04 N), using phenolphthalein as an indicator. The maximum activity was determined as 4369.75 U mL-1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%. |
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Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550Produção de lipase por meio de microrganismos isolados de solos com histórico de contato com petróleo - DOI: 10.4025/actascitechnol.v32i1.7550submerged fermentationlipolytic activitiesinducerfermentação submersaatividade lipolíticaindutorLipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as inducer. The experiments were conduced in 250 mL Erlenmeyer flasks in a shaker (170 rpm), containing 125 mL (%, w v-1) of KH2PO4, MgSO4.7H2O, NaNO3, yeast extract, peptone and starch. The pH was 5.0 and 7.0; incubation temperature was 30 and 37°C, respectively; fermentation time was 24, 48, 72, 96h, 120 and 144h; and the inducer concentration was 3 and 4%, added after 72h of fermentation. The determination of lipolytic activity was examined at 37°C, in a shaker in thermostatic bath with rotation of 82 rpm, using gum arabic 7% (w v-1), olive oil and water. The reactions were conducted for 10 min with agitation and later stopped by the addition of an acetone:ethanol:water solution, where released fatty acids were titrated with a solution of KOH (0.04 N), using phenolphthalein as an indicator. The maximum activity was determined as 4369.75 U mL-1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%.O presente trabalho tem como objetivo apresentar alternativas para a produção de enzimas lipolíticas a partir de microrganismos isolados de solos com histórico de contato com petróleo da região de Sergipe, por fermentação submersa, utilizando o óleo de palma como indutor. Os experimentos foram conduzidos em frascos erlenmeyer de 250 mL, em agitador do tipo shaker (170 rpm), contendo 125 mL (%, w v-1) de KH2PO4, MgSO4.7H2O, NaNO3, extrato de levedura, peptona e amido. O pH foi de 5,0 e 7,0; a temperatura de incubação foi de 30 e 37ºC; o tempo de fermentação, de 24, 48, 72, 96, 120 e 144h; e a proporção de indutor foi de 3 e 4%, adicionado após 72h de fermentação. A determinação da atividade lipolítica foi analisada a 37ºC, num Shaker em banho termostático com agitasção, utilizando goma arábica 7% (p v-1); azeite de oliva e água. As reações foram conduzidas durante 10 min e posteriormente interrompida pela adição de uma solução acetanólica, e titulados com uma solução de KOH (0,04 N), utilizando a fenolftaleína como indicador. A máxima atividade determinada foi de 4369,75 U mL-1 a pH 7,0 e temperatura de 37ºC, com a porcentagem do indutor óleo de palma a 4%.Universidade Estadual De Maringá2009-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/755010.4025/actascitechnol.v32i1.7550Acta Scientiarum. Technology; Vol 32 No 1 (2010); 27-31Acta Scientiarum. Technology; v. 32 n. 1 (2010); 27-311806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550/7550Feitosa, Ingrid CavalcantiBarbosa, José Murillo de PinhoOrellana, Sara CuadrosLima, Álvaro SilvaSoares, Cleide Mara Fariainfo:eu-repo/semantics/openAccess2022-11-23T18:37:38Zoai:periodicos.uem.br/ojs:article/7550Revistahttp://periodicos.uem.br/ojs/index.php/ActaSciTechnolPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2022-11-23T18:37:38Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 Produção de lipase por meio de microrganismos isolados de solos com histórico de contato com petróleo - DOI: 10.4025/actascitechnol.v32i1.7550 |
title |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
spellingShingle |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 Feitosa, Ingrid Cavalcanti submerged fermentation lipolytic activities inducer fermentação submersa atividade lipolítica indutor |
title_short |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
title_full |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
title_fullStr |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
title_full_unstemmed |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
title_sort |
Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550 |
author |
Feitosa, Ingrid Cavalcanti |
author_facet |
Feitosa, Ingrid Cavalcanti Barbosa, José Murillo de Pinho Orellana, Sara Cuadros Lima, Álvaro Silva Soares, Cleide Mara Faria |
author_role |
author |
author2 |
Barbosa, José Murillo de Pinho Orellana, Sara Cuadros Lima, Álvaro Silva Soares, Cleide Mara Faria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Feitosa, Ingrid Cavalcanti Barbosa, José Murillo de Pinho Orellana, Sara Cuadros Lima, Álvaro Silva Soares, Cleide Mara Faria |
dc.subject.por.fl_str_mv |
submerged fermentation lipolytic activities inducer fermentação submersa atividade lipolítica indutor |
topic |
submerged fermentation lipolytic activities inducer fermentação submersa atividade lipolítica indutor |
description |
Lipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as inducer. The experiments were conduced in 250 mL Erlenmeyer flasks in a shaker (170 rpm), containing 125 mL (%, w v-1) of KH2PO4, MgSO4.7H2O, NaNO3, yeast extract, peptone and starch. The pH was 5.0 and 7.0; incubation temperature was 30 and 37°C, respectively; fermentation time was 24, 48, 72, 96h, 120 and 144h; and the inducer concentration was 3 and 4%, added after 72h of fermentation. The determination of lipolytic activity was examined at 37°C, in a shaker in thermostatic bath with rotation of 82 rpm, using gum arabic 7% (w v-1), olive oil and water. The reactions were conducted for 10 min with agitation and later stopped by the addition of an acetone:ethanol:water solution, where released fatty acids were titrated with a solution of KOH (0.04 N), using phenolphthalein as an indicator. The maximum activity was determined as 4369.75 U mL-1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550 10.4025/actascitechnol.v32i1.7550 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550 |
identifier_str_mv |
10.4025/actascitechnol.v32i1.7550 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550/7550 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 32 No 1 (2010); 27-31 Acta Scientiarum. Technology; v. 32 n. 1 (2010); 27-31 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1750315281380540416 |