Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum. Animal Sciences (Online) |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6462 |
Resumo: | This study evaluated the effect of fibrolytic enzyme addition (cellulase and xylanase) on the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose in situ degradability of corn silage (CS) and rice straw (RS), using six cattle cannulated on the rumen. Acid detergent insoluble nitrogen (ADIN/total N) was evaluated. The fibrolytic enzymes were extracted from Aspergillus niger and Trichoderma longibrachiatum fungi, supplied at 0.75 g/kg of DM/animal/day, through ruminal cannula. The incubation times were 0, 3, 6, 12, 24, 48, 72 and 96 hours. The incubation residues were compared by scanning electron micrograph (SEM). Soluble fraction of DM and CP corn silage increased with enzyme supplementation. There was no effect of enzymes on the cell wall degradation of roughages. The enzyme addition did not alter the ADIN/total N level of corn silage; however, this ratio was increased on the rice straw incubated during 12 and 48 h. In general, the SEM observations showed increased bacterial colonization on the cell wall of roughages with fibrolytic enzymes addition, although without effect on ruminal degradation. |
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Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462Degradação ruminal da silagem de milho e da palha de arroz utilizando enzimas fibrolíticas exógenas - DOI: 10.4025/actascianimsci.v30i4.6462celluloseelectron micrographfibermicroorganismsrumen.celulasefibramicrorganismosmicroscopia eletrônicarúmen.This study evaluated the effect of fibrolytic enzyme addition (cellulase and xylanase) on the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose in situ degradability of corn silage (CS) and rice straw (RS), using six cattle cannulated on the rumen. Acid detergent insoluble nitrogen (ADIN/total N) was evaluated. The fibrolytic enzymes were extracted from Aspergillus niger and Trichoderma longibrachiatum fungi, supplied at 0.75 g/kg of DM/animal/day, through ruminal cannula. The incubation times were 0, 3, 6, 12, 24, 48, 72 and 96 hours. The incubation residues were compared by scanning electron micrograph (SEM). Soluble fraction of DM and CP corn silage increased with enzyme supplementation. There was no effect of enzymes on the cell wall degradation of roughages. The enzyme addition did not alter the ADIN/total N level of corn silage; however, this ratio was increased on the rice straw incubated during 12 and 48 h. In general, the SEM observations showed increased bacterial colonization on the cell wall of roughages with fibrolytic enzymes addition, although without effect on ruminal degradation.Estudou-se o efeito da adição de enzimas fibrolíticas (celulase e xilanase) sobre a degradabilidade in situ da matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA) e hemicelulose da silagem de milho (SM) e da palha de arroz (PA), utilizando seis bovinos fistulados no rúmen. Também foi avaliada a proporção de nitrogênio (N) insolúvel em detergente ácido em relação ao N total (NIDA/N total). As enzimas fibrolíticas foram extraídas dos fungos Aspergillus Níger e Trichoderma longibrachiatum, sendo fornecido 0,75 g kg-1 de MS animal-1 dia-1, via cânula ruminal. Os tempos de incubação foram: 0, 3, 6, 12, 24, 48, 72 e 96 h. Os resíduos de incubação foram comparados por meio da microscopia eletrônica de varredura (MEV). A fração solúvel da MS e da PB da silagem de milho aumentou com a adição de enzimas fibrolíticas. Não houve efeito das enzimas sobre a degradação da parede celular dos volumosos. A adição de enzimas não alterou o teor de NIDA/N total da silagem de milho, porém aumentou esta proporção nos resíduos da palha de arroz incubados durante 12 e 48 h. As observações ao MEV indicaram aumento da colonização bacteriana sobre a parede celular dos volumosos, mas sem efeito sobre a degradação ruminal.Editora da Universidade Estadual de Maringá2009-03-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/646210.4025/actascianimsci.v30i4.6462Acta Scientiarum. Animal Sciences; Vol 30 No 4 (2008); 435-442Acta Scientiarum. Animal Sciences; v. 30 n. 4 (2008); 435-4421807-86721806-2636reponame:Acta Scientiarum. Animal Sciences (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6462/6462Martins, Adriana de SouzaVieira, Paulo de FigueiredoBerchielli, Telma TeresinhaPrado, Ivanor Nunes doinfo:eu-repo/semantics/openAccess2024-05-17T13:04:09Zoai:periodicos.uem.br/ojs:article/6462Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/oaiactaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com1807-86721806-2636opendoar:2024-05-17T13:04:09Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 Degradação ruminal da silagem de milho e da palha de arroz utilizando enzimas fibrolíticas exógenas - DOI: 10.4025/actascianimsci.v30i4.6462 |
title |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
spellingShingle |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 Martins, Adriana de Souza cellulose electron micrograph fiber microorganisms rumen. celulase fibra microrganismos microscopia eletrônica rúmen. |
title_short |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
title_full |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
title_fullStr |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
title_full_unstemmed |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
title_sort |
Ruminal degradation of corn silage and rice straw using exogenous fibrolityc enzymes - DOI: 10.4025/actascianimsci.v30i4.6462 |
author |
Martins, Adriana de Souza |
author_facet |
Martins, Adriana de Souza Vieira, Paulo de Figueiredo Berchielli, Telma Teresinha Prado, Ivanor Nunes do |
author_role |
author |
author2 |
Vieira, Paulo de Figueiredo Berchielli, Telma Teresinha Prado, Ivanor Nunes do |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, Adriana de Souza Vieira, Paulo de Figueiredo Berchielli, Telma Teresinha Prado, Ivanor Nunes do |
dc.subject.por.fl_str_mv |
cellulose electron micrograph fiber microorganisms rumen. celulase fibra microrganismos microscopia eletrônica rúmen. |
topic |
cellulose electron micrograph fiber microorganisms rumen. celulase fibra microrganismos microscopia eletrônica rúmen. |
description |
This study evaluated the effect of fibrolytic enzyme addition (cellulase and xylanase) on the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose in situ degradability of corn silage (CS) and rice straw (RS), using six cattle cannulated on the rumen. Acid detergent insoluble nitrogen (ADIN/total N) was evaluated. The fibrolytic enzymes were extracted from Aspergillus niger and Trichoderma longibrachiatum fungi, supplied at 0.75 g/kg of DM/animal/day, through ruminal cannula. The incubation times were 0, 3, 6, 12, 24, 48, 72 and 96 hours. The incubation residues were compared by scanning electron micrograph (SEM). Soluble fraction of DM and CP corn silage increased with enzyme supplementation. There was no effect of enzymes on the cell wall degradation of roughages. The enzyme addition did not alter the ADIN/total N level of corn silage; however, this ratio was increased on the rice straw incubated during 12 and 48 h. In general, the SEM observations showed increased bacterial colonization on the cell wall of roughages with fibrolytic enzymes addition, although without effect on ruminal degradation. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6462 10.4025/actascianimsci.v30i4.6462 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6462 |
identifier_str_mv |
10.4025/actascianimsci.v30i4.6462 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6462/6462 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Animal Sciences; Vol 30 No 4 (2008); 435-442 Acta Scientiarum. Animal Sciences; v. 30 n. 4 (2008); 435-442 1807-8672 1806-2636 reponame:Acta Scientiarum. Animal Sciences (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Animal Sciences (Online) |
collection |
Acta Scientiarum. Animal Sciences (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com |
_version_ |
1799315359623282688 |