MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Agricultura Neotropical |
Texto Completo: | https://periodicosonline.uems.br/index.php/agrineo/article/view/3203 |
Resumo: | Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry. |
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MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANTLippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia2019-03-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicosonline.uems.br/index.php/agrineo/article/view/320310.32404/rean.v6i1.3203Revista de Agricultura Neotropical; v. 6 n. 1 (2019): Revista de Agricultura Neotropical; 1-7REVISTA DE AGRICULTURA NEOTROPICAL; Vol. 6 No. 1 (2019): REVISTA DE AGRICULTURA NEOTROPICAL; 1-72358-630310.32404/rean.v6i1reponame:Revista de Agricultura Neotropicalinstname:Universidade Estadual de Mato Grosso do Sul (UEMS)instacron:UEMSenghttps://periodicosonline.uems.br/index.php/agrineo/article/view/3203/2740Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICALinfo:eu-repo/semantics/openAccessJosé, Diego Pandelóde Campos, José Marcello SalabertViccini, Lyderson FacioAlkimim, Emilly RuasSantos, Marcelo de Oliveira2021-07-22T10:02:06Zoai:ojs2.periodicos.uems.br:article/3203Revistahttps://periodicosonline.uems.br/index.php/agrineoPUBhttps://periodicosonline.uems.br/index.php/agrineo/oaiagriculturaneotropical@uems.br || wellingthon.junnyor@uems.br2358-63032358-6303opendoar:2022-05-16T12:53:13.043408Revista de Agricultura Neotropical - Universidade Estadual de Mato Grosso do Sul (UEMS)false |
dc.title.none.fl_str_mv |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
title |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
spellingShingle |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT José, Diego Pandeló |
title_short |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
title_full |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
title_fullStr |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
title_full_unstemmed |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
title_sort |
MICROPROPAGATION AND PLOIDY STABILITY OF Lippia lacunosa Mart. & Schauer: AN ENDANGERED BRAZILIAN MEDICINAL PLANT |
author |
José, Diego Pandeló |
author_facet |
José, Diego Pandeló de Campos, José Marcello Salabert Viccini, Lyderson Facio Alkimim, Emilly Ruas Santos, Marcelo de Oliveira |
author_role |
author |
author2 |
de Campos, José Marcello Salabert Viccini, Lyderson Facio Alkimim, Emilly Ruas Santos, Marcelo de Oliveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
José, Diego Pandeló de Campos, José Marcello Salabert Viccini, Lyderson Facio Alkimim, Emilly Ruas Santos, Marcelo de Oliveira |
description |
Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicosonline.uems.br/index.php/agrineo/article/view/3203 10.32404/rean.v6i1.3203 |
url |
https://periodicosonline.uems.br/index.php/agrineo/article/view/3203 |
identifier_str_mv |
10.32404/rean.v6i1.3203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicosonline.uems.br/index.php/agrineo/article/view/3203/2740 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICAL info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 REVISTA DE AGRICULTURA NEOTROPICAL |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia |
publisher.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Cassilândia |
dc.source.none.fl_str_mv |
Revista de Agricultura Neotropical; v. 6 n. 1 (2019): Revista de Agricultura Neotropical; 1-7 REVISTA DE AGRICULTURA NEOTROPICAL; Vol. 6 No. 1 (2019): REVISTA DE AGRICULTURA NEOTROPICAL; 1-7 2358-6303 10.32404/rean.v6i1 reponame:Revista de Agricultura Neotropical instname:Universidade Estadual de Mato Grosso do Sul (UEMS) instacron:UEMS |
instname_str |
Universidade Estadual de Mato Grosso do Sul (UEMS) |
instacron_str |
UEMS |
institution |
UEMS |
reponame_str |
Revista de Agricultura Neotropical |
collection |
Revista de Agricultura Neotropical |
repository.name.fl_str_mv |
Revista de Agricultura Neotropical - Universidade Estadual de Mato Grosso do Sul (UEMS) |
repository.mail.fl_str_mv |
agriculturaneotropical@uems.br || wellingthon.junnyor@uems.br |
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1797051251415842816 |