Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/3177 |
Resumo: | The objective of this study was to analyze the antifungal potential of the Syzygium cumini (Linn.) Skeels leaves’ hydroalcoholic extract on strains of the Candida genre, as well as to investigate its cytotoxic and antioxidant effect. The percolation method was used to obtain the extract. The extract was chemically characterized through gas chromatography combined with mass spectrometry. The extract’s antifungal activity was determined through Minimum Inhibitory Concentration and Minimum Fungicide Concentration over C. albicans (ATCC 10231), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), C. tropicalis (ATCC 750) ) as well as C. albicans (LM1) and C. albicans (LM3) clinical strains and through the evaluation of its effect over the C. albicans (ATCC 10231) growth kinetics. Cytotoxicity tests were done from the hemolysis and osmotic fragility experiments in human erythrocytes as well as the extract’s oxidant and antioxidant potentials in the presence of phenylhydrazine and reactive oxygen species, respectively. The data about the extract’s essay over C. albicans growth kinetics were evaluated by the ANOVA test and by the post-tests of Tukey, while the cytotoxity tests were evaluated by the ANOVA test and by the post-tests of Dunnett. Both were done taking into consideration the value α=0,05. By the phytochemical analysis, a total of 35 compounds were identified, knowing that 2,2- Dimethoxybutane; 4H-Pyran-4-one,2,3-di-hydro-3,5-di-hydroxy-6-methyl-; 5-Hydroxymethylfurfural; 2,4-Di-hydroxy-2,5-dimethyl-3 (2H) -furan-3-one, propanedioic acid and dimethyl ester were the main substances found in the extract. The extract showed MIC values among 31,25 and 125 μg/mL and MFC among 250 and >1000 μg/mL, with fungistatic effect over the tested strains, mainly after they were exposed to the C. albicans for a period of eight hours. In those concentrations that can have a minimum inhibitory effect over the fungal growth, the extract showed a low hemolytic activity, having a protective effect over osmotic stress induced hemolysis, at the same concentrations, depending on the blood type used. In concentrations of 31,25 and 62,5 μg/mL, the extract shows an antioxidant effect in relation to oxidative stress induced by hydrogen peroxide, though it does not demonstrate any antioxidant action when exposed to phenylhydrazine. Besides, it does not induce oxidation in the erythrocytes when used in concentrations ≤ 125 μg/mL. Based on these results, the extract showed a promising antifungal potential, manifesting a low cytotoxic effect over the erythrocytes, offering a protective effect over cells that were exposed to osmotic and oxidative stress situations. |
id |
UEPB_05caa4e8a12dd362f8a142150c397e07 |
---|---|
oai_identifier_str |
oai:tede.bc.uepb.edu.br:tede/3177 |
network_acronym_str |
UEPB |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEPB |
repository_id_str |
|
spelling |
Pereira, Jozinete Vieira46831576400http://lattes.cnpq.br/8041850574045487Medeiros, Ana Claudia Dantas de92956467468http://lattes.cnpq.br/0640671064146120Cavalcanti, Yuri Wanderley06637446440http://lattes.cnpq.br/130369520544280207837886496http://lattes.cnpq.br/4286434824060449Figueirêdo Júnior, Ernani Canuto2019-02-18T14:24:10Z2017-06-20FIGUEIRÊDO JÚNIOR, E. C. Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel. 2017. 130f. Dissertação (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande, 2017.http://tede.bc.uepb.edu.br/jspui/handle/tede/3177The objective of this study was to analyze the antifungal potential of the Syzygium cumini (Linn.) Skeels leaves’ hydroalcoholic extract on strains of the Candida genre, as well as to investigate its cytotoxic and antioxidant effect. The percolation method was used to obtain the extract. The extract was chemically characterized through gas chromatography combined with mass spectrometry. The extract’s antifungal activity was determined through Minimum Inhibitory Concentration and Minimum Fungicide Concentration over C. albicans (ATCC 10231), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), C. tropicalis (ATCC 750) ) as well as C. albicans (LM1) and C. albicans (LM3) clinical strains and through the evaluation of its effect over the C. albicans (ATCC 10231) growth kinetics. Cytotoxicity tests were done from the hemolysis and osmotic fragility experiments in human erythrocytes as well as the extract’s oxidant and antioxidant potentials in the presence of phenylhydrazine and reactive oxygen species, respectively. The data about the extract’s essay over C. albicans growth kinetics were evaluated by the ANOVA test and by the post-tests of Tukey, while the cytotoxity tests were evaluated by the ANOVA test and by the post-tests of Dunnett. Both were done taking into consideration the value α=0,05. By the phytochemical analysis, a total of 35 compounds were identified, knowing that 2,2- Dimethoxybutane; 4H-Pyran-4-one,2,3-di-hydro-3,5-di-hydroxy-6-methyl-; 5-Hydroxymethylfurfural; 2,4-Di-hydroxy-2,5-dimethyl-3 (2H) -furan-3-one, propanedioic acid and dimethyl ester were the main substances found in the extract. The extract showed MIC values among 31,25 and 125 μg/mL and MFC among 250 and >1000 μg/mL, with fungistatic effect over the tested strains, mainly after they were exposed to the C. albicans for a period of eight hours. In those concentrations that can have a minimum inhibitory effect over the fungal growth, the extract showed a low hemolytic activity, having a protective effect over osmotic stress induced hemolysis, at the same concentrations, depending on the blood type used. In concentrations of 31,25 and 62,5 μg/mL, the extract shows an antioxidant effect in relation to oxidative stress induced by hydrogen peroxide, though it does not demonstrate any antioxidant action when exposed to phenylhydrazine. Besides, it does not induce oxidation in the erythrocytes when used in concentrations ≤ 125 μg/mL. Based on these results, the extract showed a promising antifungal potential, manifesting a low cytotoxic effect over the erythrocytes, offering a protective effect over cells that were exposed to osmotic and oxidative stress situations.O objetivo desse estudo foi avaliar o potencial antifúngico do extrato hidroalcoólico de folhas de Syzygium cumini (Linn.) Skeels sobre cepas do gênero Candida, bem como investigar o efeito citotóxico e antioxidante. O método de extração utilizado para obtenção do extrato foi a percolação. O extrato foi quimicamente caracterizado por meio de cromatografia gasosa associada a espectrometria de massa. A atividade antifúngica do extrato foi determinada por meio da Concentração Inibitória Mínima, Concentração Fungicida Mínima sobre C. albicans (ATCC 10231), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), C. tropicalis (ATCC 750) e cepas clínicas de C. albicans (LM1) e C. albicans (LM3) e da avaliação do seu efeito sobre a cinética de crescimento de C. albicans (ATCC 10231). Testes de citotoxicidade foram realizados a partir dos ensaios de hemólise e fragilidade osmótica em eritrócitos humanos, além da avaliação do seu potencial oxidante e antioxidante na presença de fenilhidrazina e espécies reativas de oxigênio, respectivamente. Os dados do ensaio do extrato sobre a cinética de crescimento de C. albicans foram avaliados através do teste ANOVA seguido pelo pós-teste de Tukey, enquanto os testes de citotoxicidade foram avaliados através do teste ANOVA seguido pelo pós- teste de Dunnett. Ambos foram realizados admitindo-se um valor α=0,05. Mediante a análise fitoquímica, um total de 35 compostos foram identificados, sendo 2,2- Dimetoxibutano;4H-Piran-4-ona, 2,3-di-hidro-3,5-di-hidroxi-6-metil-; 5-Hidroximetilfurfural; 2,4-Di-hidroxi-2,5-dimetil-3 (2H) -furan-3-ona e Ácido propanodioico, dimetil éster as substâncias majoritárias presentes no extrato. O extrato demonstrou valores de CIM entre 31,25 e 125 μg/mL e de CFM entre 250 e >1000 μg/mL, com efeito fungistático sobre as cepas testadas, sendo esse mais significativo em um período de oito horas de exposição à C. albicans. Nas concentrações capazes de exercer efeito inibitório mínimo sobre o crescimento fúngico, o extrato demonstrou baixa atividade hemolítica, exercendo efeito protetor sobre a hemólise induzida por estresse osmótico quando utilizado nessas mesmas concentrações, de acordo com o tipo sanguíneo. Em concentrações de 31,25 e 62,5 μg/mL o extrato demonstrou efeito antioxidante frente a estresse oxidativo induzido pelo peróxido de hidrogênio, apesar de não demonstrar ação antioxidante frente a fenilhidrazina. Além disso, sua utilização em concentrações ≤ 125 μg/mL não induz a ocorrência de oxidação nos eritrócitos. Diante dos resultados obtidos, o extrato demonstrou potencial antifúngico promissor, apresentando baixo efeito citotóxico sobre eritrócitos, oferecendo efeito protetor sobre células expostas a situações de estresse osmótico e oxidativo.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2018-11-23T14:59:40Z No. of bitstreams: 1 PDF - Ernani Canuto Figueirêdo Júnior.pdf: 44903202 bytes, checksum: 904f463fa51a77c97b6bc8a7d217c759 (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2019-02-18T14:24:10Z (GMT) No. of bitstreams: 1 PDF - Ernani Canuto Figueirêdo Júnior.pdf: 44903202 bytes, checksum: 904f463fa51a77c97b6bc8a7d217c759 (MD5)Made available in DSpace on 2019-02-18T14:24:10Z (GMT). No. of bitstreams: 1 PDF - Ernani Canuto Figueirêdo Júnior.pdf: 44903202 bytes, checksum: 904f463fa51a77c97b6bc8a7d217c759 (MD5) Previous issue date: 2017-06-20Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.bc.uepb.edu.br/jspui/retrieve/7541/PDF%20-%20Ernani%20Canuto%20Figueir%c3%aado%20J%c3%banior.pdf.jpgporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Odontologia - PPGOUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPToxicidadeAntifúngicosCandidose bucalFitoterapiaAntifungalToxicityCIENCIAS DA SAUDE::ODONTOLOGIAProspecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis188746850794111162600600600524871450381110278-2070498469879244349info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBTHUMBNAILPDF - Ernani Canuto Figueirêdo Júnior.pdf.jpgPDF - Ernani Canuto Figueirêdo Júnior.pdf.jpgimage/jpeg3898http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/4/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf.jpg4a8a81aad727de75ee5ebb50bdc23527MD54TEXTPDF - Ernani Canuto Figueirêdo Júnior.pdf.txtPDF - Ernani Canuto Figueirêdo Júnior.pdf.txttext/plain132http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/3/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf.txtbf6517c8752a19cbb92ac7857100ed42MD53ORIGINALPDF - Ernani Canuto Figueirêdo Júnior.pdfPDF - Ernani Canuto Figueirêdo Júnior.pdfapplication/pdf44903202http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/2/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf904f463fa51a77c97b6bc8a7d217c759MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/31772019-02-19 01:27:14.375oai:tede.bc.uepb.edu.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2019-02-19T04:27:14Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
title |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
spellingShingle |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel Figueirêdo Júnior, Ernani Canuto Toxicidade Antifúngicos Candidose bucal Fitoterapia Antifungal Toxicity CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
title_full |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
title_fullStr |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
title_full_unstemmed |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
title_sort |
Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel |
author |
Figueirêdo Júnior, Ernani Canuto |
author_facet |
Figueirêdo Júnior, Ernani Canuto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Pereira, Jozinete Vieira |
dc.contributor.advisor1ID.fl_str_mv |
46831576400 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8041850574045487 |
dc.contributor.referee1.fl_str_mv |
Medeiros, Ana Claudia Dantas de |
dc.contributor.referee1ID.fl_str_mv |
92956467468 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0640671064146120 |
dc.contributor.referee2.fl_str_mv |
Cavalcanti, Yuri Wanderley |
dc.contributor.referee2ID.fl_str_mv |
06637446440 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1303695205442802 |
dc.contributor.authorID.fl_str_mv |
07837886496 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4286434824060449 |
dc.contributor.author.fl_str_mv |
Figueirêdo Júnior, Ernani Canuto |
contributor_str_mv |
Pereira, Jozinete Vieira Medeiros, Ana Claudia Dantas de Cavalcanti, Yuri Wanderley |
dc.subject.por.fl_str_mv |
Toxicidade Antifúngicos Candidose bucal Fitoterapia |
topic |
Toxicidade Antifúngicos Candidose bucal Fitoterapia Antifungal Toxicity CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Antifungal Toxicity |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The objective of this study was to analyze the antifungal potential of the Syzygium cumini (Linn.) Skeels leaves’ hydroalcoholic extract on strains of the Candida genre, as well as to investigate its cytotoxic and antioxidant effect. The percolation method was used to obtain the extract. The extract was chemically characterized through gas chromatography combined with mass spectrometry. The extract’s antifungal activity was determined through Minimum Inhibitory Concentration and Minimum Fungicide Concentration over C. albicans (ATCC 10231), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), C. tropicalis (ATCC 750) ) as well as C. albicans (LM1) and C. albicans (LM3) clinical strains and through the evaluation of its effect over the C. albicans (ATCC 10231) growth kinetics. Cytotoxicity tests were done from the hemolysis and osmotic fragility experiments in human erythrocytes as well as the extract’s oxidant and antioxidant potentials in the presence of phenylhydrazine and reactive oxygen species, respectively. The data about the extract’s essay over C. albicans growth kinetics were evaluated by the ANOVA test and by the post-tests of Tukey, while the cytotoxity tests were evaluated by the ANOVA test and by the post-tests of Dunnett. Both were done taking into consideration the value α=0,05. By the phytochemical analysis, a total of 35 compounds were identified, knowing that 2,2- Dimethoxybutane; 4H-Pyran-4-one,2,3-di-hydro-3,5-di-hydroxy-6-methyl-; 5-Hydroxymethylfurfural; 2,4-Di-hydroxy-2,5-dimethyl-3 (2H) -furan-3-one, propanedioic acid and dimethyl ester were the main substances found in the extract. The extract showed MIC values among 31,25 and 125 μg/mL and MFC among 250 and >1000 μg/mL, with fungistatic effect over the tested strains, mainly after they were exposed to the C. albicans for a period of eight hours. In those concentrations that can have a minimum inhibitory effect over the fungal growth, the extract showed a low hemolytic activity, having a protective effect over osmotic stress induced hemolysis, at the same concentrations, depending on the blood type used. In concentrations of 31,25 and 62,5 μg/mL, the extract shows an antioxidant effect in relation to oxidative stress induced by hydrogen peroxide, though it does not demonstrate any antioxidant action when exposed to phenylhydrazine. Besides, it does not induce oxidation in the erythrocytes when used in concentrations ≤ 125 μg/mL. Based on these results, the extract showed a promising antifungal potential, manifesting a low cytotoxic effect over the erythrocytes, offering a protective effect over cells that were exposed to osmotic and oxidative stress situations. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-06-20 |
dc.date.accessioned.fl_str_mv |
2019-02-18T14:24:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FIGUEIRÊDO JÚNIOR, E. C. Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel. 2017. 130f. Dissertação (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande, 2017. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3177 |
identifier_str_mv |
FIGUEIRÊDO JÚNIOR, E. C. Prospecção dos efeitos antifúngico, citotóxico e anti oxidante do extrato de Syzygium cumini (LINN.) Skeel. 2017. 130f. Dissertação (Programa de Pós-Graduação em Odontologia - PPGO) - Universidade Estadual da Paraíba, Campina Grande, 2017. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3177 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
188746850794111162 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
524871450381110278 |
dc.relation.cnpq.fl_str_mv |
-2070498469879244349 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Odontologia - PPGO |
dc.publisher.initials.fl_str_mv |
UEPB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
publisher.none.fl_str_mv |
Universidade Estadual da Paraíba |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEPB instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
instname_str |
Universidade Estadual da Paraíba (UEPB) |
instacron_str |
UEPB |
institution |
UEPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEPB |
collection |
Biblioteca Digital de Teses e Dissertações da UEPB |
bitstream.url.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/4/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf.jpg http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/3/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf.txt http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/2/PDF+-+Ernani+Canuto+Figueir%C3%AAdo+J%C3%BAnior.pdf http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3177/1/license.txt |
bitstream.checksum.fl_str_mv |
4a8a81aad727de75ee5ebb50bdc23527 bf6517c8752a19cbb92ac7857100ed42 904f463fa51a77c97b6bc8a7d217c759 6052ae61e77222b2086e666b7ae213ce |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB) |
repository.mail.fl_str_mv |
bc@uepb.edu.br|| |
_version_ |
1800227412173127680 |