Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/3531 |
Resumo: | Skin cancer is currently the most common type in the world, accounting for 33% of cancer cases in Brazil. Thus, in order to prevent skin cancer, premature aging and other inconveniences related to exposure to UV radiation, there is growing interest and research on photoprotectors. However, the risk of photodegradation, skin permeation and systemic absorption of these products diminishes their safety. In addition, the high lipophilicity of conventional sunscreens and low skin permanence lead to improper use, reducing effectiveness. An alternative to overcoming such deadlocks is the incorporation of sunscreens into nanostructured lipid carriers (NLC), which are aqueous dispersions of nanoparticles formed by mixtures of solid and liquid lipids. In this study, NLC containing the organic UV filter bemotrizinol (BEMT), which has a broad absorption spectrum and increased photostability, was developed and characterized using carnauba wax (solid lipid) and caprylic and caprylic acid triglyceride (liquid lipid) by ultrasound technique. A Box-Behnken experimental design was performed to optimize the NLC and evaluate the influence of three independent variables on particle size, so the ideal system was chosen for characterization. The particle size was evaluated using the dynamic light scattering (DLS) technique and decreased as the surfactant concentration increased, being analyzed up to 30 days of storage, presenting at 122.4 ± 0.3 nm on the last day. Scanning electron microscopy showed spherical particles, intact and without clusters. Analyzes of thermogravimetry, differential exploratory calorimetry, and Fourier transform infrared spectroscopy confirm the incorporation of sunscreen in nanocarriers. X-ray diffraction allows observing the reduction of crystallinity in the NLC, desired result for the nanosystem. Qualitative and quantitative in vitro analyzes performed by ultraviolet spectroscopy indicated that an increase in sunscreen photoprotective activity was observed when incorporated into the NLC. Thus, the results suggest that the developed NLC can be an innovative, promising, stable and biocompatible system for use in sunscreens. |
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Damasceno, Bolívar Ponciano Goulart de Lima91616751487http://lattes.cnpq.br/6407334157973308Oshiro Junior, Joao Augusto32661123825http://lattes.cnpq.br/7718234509377672Guimarães, Geovani Pereira06309855450http://lattes.cnpq.br/9377892025897596Formiga, Fábio Rocha00986611409http://lattes.cnpq.br/935688210165352610727153412http://lattes.cnpq.br/1987367321820890Medeiros, Thayse Silva2019-10-31T11:49:00Z2019-08-23MEDEIROS, T. S. Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol. 2019. 63f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2019.http://tede.bc.uepb.edu.br/jspui/handle/tede/3531Skin cancer is currently the most common type in the world, accounting for 33% of cancer cases in Brazil. Thus, in order to prevent skin cancer, premature aging and other inconveniences related to exposure to UV radiation, there is growing interest and research on photoprotectors. However, the risk of photodegradation, skin permeation and systemic absorption of these products diminishes their safety. In addition, the high lipophilicity of conventional sunscreens and low skin permanence lead to improper use, reducing effectiveness. An alternative to overcoming such deadlocks is the incorporation of sunscreens into nanostructured lipid carriers (NLC), which are aqueous dispersions of nanoparticles formed by mixtures of solid and liquid lipids. In this study, NLC containing the organic UV filter bemotrizinol (BEMT), which has a broad absorption spectrum and increased photostability, was developed and characterized using carnauba wax (solid lipid) and caprylic and caprylic acid triglyceride (liquid lipid) by ultrasound technique. A Box-Behnken experimental design was performed to optimize the NLC and evaluate the influence of three independent variables on particle size, so the ideal system was chosen for characterization. The particle size was evaluated using the dynamic light scattering (DLS) technique and decreased as the surfactant concentration increased, being analyzed up to 30 days of storage, presenting at 122.4 ± 0.3 nm on the last day. Scanning electron microscopy showed spherical particles, intact and without clusters. Analyzes of thermogravimetry, differential exploratory calorimetry, and Fourier transform infrared spectroscopy confirm the incorporation of sunscreen in nanocarriers. X-ray diffraction allows observing the reduction of crystallinity in the NLC, desired result for the nanosystem. Qualitative and quantitative in vitro analyzes performed by ultraviolet spectroscopy indicated that an increase in sunscreen photoprotective activity was observed when incorporated into the NLC. Thus, the results suggest that the developed NLC can be an innovative, promising, stable and biocompatible system for use in sunscreens.O câncer de pele representa, atualmente, o tipo mais comum no mundo, abrangendo 33% dos casos de câncer no Brasil. Deste modo, com o objetivo de prevenir o câncer de pele, o envelhecimento precoce e outros inconvenientes relacionados à exposição à radiação UV, é crescente o interesse e pesquisas sobre fotoprotetores. Porém, o risco de fotodegradação, permeação cutânea e absorção sistêmica desses produtos diminuem sua segurança. Além disso, a alta lipofilia dos filtros solares convencionais e baixa permanência na pele levam ao seu uso inadequado, reduzindo a eficácia. Uma alternativa para ultrapassar tais impasses é a incorporação de filtros solares em carreadores lipídicos nanoestruturados (CLN), que são dispersões aquosas de nanopartículas formadas por misturas de lipídios sólidos e líquidos. Neste estudo, CLN contendo o filtro solar orgânico bemotrizinol (BEMT), que apresenta amplo espectro de absorção e fotoestabilidade aumentada, foram desenvolvidos e carcaterizados utilizando a cera de carnaúba (lipídio sólido) e o triglicerídio do ácido cáprico e caprílico (lipídio líquido), através da técnica de ultrassonicação. Um planejamento experimental do tipo Box-Behnken foi realizado para otimizar os CLN e avaliar a influência de três variáveis independentes sobre o tamanho das partículas, então o sistema ideal foi escolhido para caracterização. O tamanho das partículas foi avaliado utilizando a técnica de espalhamento de luz dinâmico (DLS), e diminuíu à medida que a concentração de tensoativos aumentou, sendo analisado até 30 dias de armazenamento, apresentando-se em 122,4 ± 0,3 nm no último dia. A microscopia eletrônica de varredura mostrou partículas esféricas, íntegras e sem aglomerados. As análises de termogravimetria, calorimetria exploratória diferencial, e a espectroscopia na região de infravermelho com transformada de Fourier confirmam a incorporação do filtro solar nos nanocarreadores. A difração de raios-X permitiu a observação da redução da cristalinidade nos CLN, resultado desejado para o nanossistema. Análises in vitro qualitativa e quantitativa realizadas por espectroscopia na região ultravioleta indicaram que um aumento da atividade fotoprotetora do filtro solar foi observado quando incorporado aos CLN. Deste modo, os resultados sugerem que o CLN desenvolvido pode ser um sistema inovador, promissor, estável e biocompatível para o uso em fotoprotetores.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2019-10-21T16:51:11Z No. of bitstreams: 1 PDF - Thayse Silva Medeiros.pdf: 3463306 bytes, checksum: 4cacb08f2b4f2afae03d558c9e33a2b8 (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2019-10-31T11:49:00Z (GMT) No. of bitstreams: 1 PDF - Thayse Silva Medeiros.pdf: 3463306 bytes, checksum: 4cacb08f2b4f2afae03d558c9e33a2b8 (MD5)Made available in DSpace on 2019-10-31T11:49:00Z (GMT). No. of bitstreams: 1 PDF - Thayse Silva Medeiros.pdf: 3463306 bytes, checksum: 4cacb08f2b4f2afae03d558c9e33a2b8 (MD5) Previous issue date: 2019-08-23Programa de Incentivo à Pós-Graduação e Pesquisa - PROPESQapplication/pdfhttp://tede.bc.uepb.edu.br/jspui/retrieve/8992/PDF%20-%20Thayse%20Silva%20Medeiros.pdf.jpgporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Ciências Farmacêuticas - PPGCFUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPProtetor solarRadiação solar ultravioletaNanotecnologiaFotoproteçãoPhotoprotectionNanotechnologyUltraviolet solar radiationCIENCIAS DA SAUDE::FARMACIACarreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinolinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis81579685552518768746006006005248714503811102786997636413449754996info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBTHUMBNAILPDF - Thayse Silva Medeiros.pdf.jpgPDF - Thayse Silva Medeiros.pdf.jpgimage/jpeg3243http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3531/4/PDF+-+Thayse+Silva+Medeiros.pdf.jpg1948eac7e6ead476dd964710c7479233MD54TEXTPDF - Thayse Silva Medeiros.pdf.txtPDF - Thayse Silva Medeiros.pdf.txttext/plain108133http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3531/3/PDF+-+Thayse+Silva+Medeiros.pdf.txt9ea7b2e7a179362cf694e455af23e32eMD53ORIGINALPDF - Thayse Silva Medeiros.pdfPDF - Thayse Silva Medeiros.pdfapplication/pdf3463306http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3531/2/PDF+-+Thayse+Silva+Medeiros.pdf4cacb08f2b4f2afae03d558c9e33a2b8MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/3531/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/35312019-11-01 01:08:33.754oai:tede.bc.uepb.edu.br:tede/3531TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlIApFc3RhZHVhbCBkYSBQYXJhw61iYSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrdXAgZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIMOpIG9yaWdpbmFsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIApuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldSAKY29uaGVjaW1lbnRvLCBpbmZyaW5nZSBkaXJlaXRvcyBhdXRvcmFpcyBkZSBuaW5ndcOpbS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIGNvbnRlbmhhIG1hdGVyaWFsIHF1ZSB2b2PDqiBuw6NvIHBvc3N1aSBhIHRpdHVsYXJpZGFkZSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHZvY8OqIApkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVUVQQiAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRVBCLCAKVk9Dw4ogREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklTw4NPIENPTU8gVEFNQsOJTSBBUyAKREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRVBCIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKSAKZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28sIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbywgYWzDqW0gZGFxdWVsYXMgCmNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2019-11-01T04:08:33Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
title |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
spellingShingle |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol Medeiros, Thayse Silva Protetor solar Radiação solar ultravioleta Nanotecnologia Fotoproteção Photoprotection Nanotechnology Ultraviolet solar radiation CIENCIAS DA SAUDE::FARMACIA |
title_short |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
title_full |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
title_fullStr |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
title_full_unstemmed |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
title_sort |
Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol |
author |
Medeiros, Thayse Silva |
author_facet |
Medeiros, Thayse Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Damasceno, Bolívar Ponciano Goulart de Lima |
dc.contributor.advisor1ID.fl_str_mv |
91616751487 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6407334157973308 |
dc.contributor.advisor-co1.fl_str_mv |
Oshiro Junior, Joao Augusto |
dc.contributor.advisor-co1ID.fl_str_mv |
32661123825 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7718234509377672 |
dc.contributor.referee1.fl_str_mv |
Guimarães, Geovani Pereira |
dc.contributor.referee1ID.fl_str_mv |
06309855450 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9377892025897596 |
dc.contributor.referee2.fl_str_mv |
Formiga, Fábio Rocha |
dc.contributor.referee2ID.fl_str_mv |
00986611409 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9356882101653526 |
dc.contributor.authorID.fl_str_mv |
10727153412 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1987367321820890 |
dc.contributor.author.fl_str_mv |
Medeiros, Thayse Silva |
contributor_str_mv |
Damasceno, Bolívar Ponciano Goulart de Lima Oshiro Junior, Joao Augusto Guimarães, Geovani Pereira Formiga, Fábio Rocha |
dc.subject.por.fl_str_mv |
Protetor solar Radiação solar ultravioleta Nanotecnologia Fotoproteção |
topic |
Protetor solar Radiação solar ultravioleta Nanotecnologia Fotoproteção Photoprotection Nanotechnology Ultraviolet solar radiation CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Photoprotection Nanotechnology Ultraviolet solar radiation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Skin cancer is currently the most common type in the world, accounting for 33% of cancer cases in Brazil. Thus, in order to prevent skin cancer, premature aging and other inconveniences related to exposure to UV radiation, there is growing interest and research on photoprotectors. However, the risk of photodegradation, skin permeation and systemic absorption of these products diminishes their safety. In addition, the high lipophilicity of conventional sunscreens and low skin permanence lead to improper use, reducing effectiveness. An alternative to overcoming such deadlocks is the incorporation of sunscreens into nanostructured lipid carriers (NLC), which are aqueous dispersions of nanoparticles formed by mixtures of solid and liquid lipids. In this study, NLC containing the organic UV filter bemotrizinol (BEMT), which has a broad absorption spectrum and increased photostability, was developed and characterized using carnauba wax (solid lipid) and caprylic and caprylic acid triglyceride (liquid lipid) by ultrasound technique. A Box-Behnken experimental design was performed to optimize the NLC and evaluate the influence of three independent variables on particle size, so the ideal system was chosen for characterization. The particle size was evaluated using the dynamic light scattering (DLS) technique and decreased as the surfactant concentration increased, being analyzed up to 30 days of storage, presenting at 122.4 ± 0.3 nm on the last day. Scanning electron microscopy showed spherical particles, intact and without clusters. Analyzes of thermogravimetry, differential exploratory calorimetry, and Fourier transform infrared spectroscopy confirm the incorporation of sunscreen in nanocarriers. X-ray diffraction allows observing the reduction of crystallinity in the NLC, desired result for the nanosystem. Qualitative and quantitative in vitro analyzes performed by ultraviolet spectroscopy indicated that an increase in sunscreen photoprotective activity was observed when incorporated into the NLC. Thus, the results suggest that the developed NLC can be an innovative, promising, stable and biocompatible system for use in sunscreens. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-10-31T11:49:00Z |
dc.date.issued.fl_str_mv |
2019-08-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEDEIROS, T. S. Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol. 2019. 63f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3531 |
identifier_str_mv |
MEDEIROS, T. S. Carreadores lipídicos nanoestruturados como estratégia para incorporação do filtro solar Bemotrizinol. 2019. 63f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF) - Universidade Estadual da Paraíba, Campina Grande, 2019. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/3531 |
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por |
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por |
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8157968555251876874 |
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600 600 600 |
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524871450381110278 |
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6997636413449754996 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual da Paraíba |
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Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF |
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UEPB |
dc.publisher.country.fl_str_mv |
Brasil |
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Pró-Reitoria de Pós-Graduação e Pesquisa - PRPGP |
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Universidade Estadual da Paraíba |
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