Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/20943 |
Resumo: | The clinical features observed in cases of classic diphtheria caused by Corynebacterium diphtheriae are associated with diphtheria toxin production (DT), its main virulence factor. However, non-DT-producing strains have been isolated from invasive infections. Recent studies have demonstrated the involvement of a multifactorial virulence mechanism expressed by C. diphtheriae strains. The analysis of the pangenome of this species identified the presence of 57 genomic islands, indicating that horizontal gene transfer is a strategy frequently used by corynebacteria to establish differences in pathogen-host interactions. Several studies indicate that the genes encoding Shiga toxin are transferable between species. In Corynebacterium ulcerans, the Rbp protein, similar to the A chain of Escherichia coli Shiga toxin (SLT-1), whose function is to inhibit the synthesis of ribosomes causing cell death, was previously identified. Additionally, genes encoding hemolysins, another important virulence factor, may cause cell damage through pore formation. The alpha-hemolysin (HlyA) genes from E. coli and other bacterial species are also capable of horizontal transfer. Some C. diphtheriae strains possibly produce specific hemolysins, as they are classified according to hemolytic activity. Genomic sequencing of C. diphtheriae biotype gravis NCTC 13129 strain identified the sequence of a hypothetical hemolysin-like protein (TlyA) from Mycobacterium tuberculosis. Therefore, the presence and expression of genes similar to Shiga-like toxins and hemolysin were demonstrated in different Corynebacterium species. Additionally, the following experiments for the investigation of phenotypic expression were performed: i) construction of mutant strains for the target genes: ii) gene expression by real-time PCR; iii) Vero cell cytotoxicity assays; iv) influence of environmental conditions on the expressions of these genes; v) arthritogenic potential using an animal model (murine). Data obtained through bioinformatics analysis showed that different Corynebacterium species presented genes that encode proteins with expressive homology to the toxins Rbp (Shiga-like toxin) and TlyA (hemolysin) toxins. Phenotypic assays verified the cytotoxic effects of these proteins on different C. diphtheriae strains. In conclusion, C. diphtheriae strains isolated from cases of diphtheria and/or invasive infections may also utilize the production of TlyA hemolysin and Shiga-like toxin in the strain-dependent mechanisms of pathogenicity. Additional studies related to these virulence factors remain ongoing for both C. diphtheriae and other clinically relevant corynebacteria |
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Guaraldi, Ana Luíza de Mattoshttp://lattes.cnpq.br/8091118564093203Saliba, Alessandra Mattoshttp://lattes.cnpq.br/6134920105834158Ignacio, Ana Claudia de Paula Rosahttp://lattes.cnpq.br/7809403473011306Sant'Anna, Louisy Sanches dos Santoshttp://lattes.cnpq.br/5999066009401057Gomes, Débora Leandro Ramahttp://lattes.cnpq.br/4860403157095790Neves, Felipe Piedade Gonçalveshttp://lattes.cnpq.br/5289973921789746http://lattes.cnpq.br/6918848605710038Cucinelli, Andrezza do Espírito Santoandrezzacucinelli@yahoo.com.br2024-01-17T19:09:32Z2022-06-29CUCINELLI, Andrezza do Espírito Santo. Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência. 2022. 171 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2022.http://www.bdtd.uerj.br/handle/1/20943The clinical features observed in cases of classic diphtheria caused by Corynebacterium diphtheriae are associated with diphtheria toxin production (DT), its main virulence factor. However, non-DT-producing strains have been isolated from invasive infections. Recent studies have demonstrated the involvement of a multifactorial virulence mechanism expressed by C. diphtheriae strains. The analysis of the pangenome of this species identified the presence of 57 genomic islands, indicating that horizontal gene transfer is a strategy frequently used by corynebacteria to establish differences in pathogen-host interactions. Several studies indicate that the genes encoding Shiga toxin are transferable between species. In Corynebacterium ulcerans, the Rbp protein, similar to the A chain of Escherichia coli Shiga toxin (SLT-1), whose function is to inhibit the synthesis of ribosomes causing cell death, was previously identified. Additionally, genes encoding hemolysins, another important virulence factor, may cause cell damage through pore formation. The alpha-hemolysin (HlyA) genes from E. coli and other bacterial species are also capable of horizontal transfer. Some C. diphtheriae strains possibly produce specific hemolysins, as they are classified according to hemolytic activity. Genomic sequencing of C. diphtheriae biotype gravis NCTC 13129 strain identified the sequence of a hypothetical hemolysin-like protein (TlyA) from Mycobacterium tuberculosis. Therefore, the presence and expression of genes similar to Shiga-like toxins and hemolysin were demonstrated in different Corynebacterium species. Additionally, the following experiments for the investigation of phenotypic expression were performed: i) construction of mutant strains for the target genes: ii) gene expression by real-time PCR; iii) Vero cell cytotoxicity assays; iv) influence of environmental conditions on the expressions of these genes; v) arthritogenic potential using an animal model (murine). Data obtained through bioinformatics analysis showed that different Corynebacterium species presented genes that encode proteins with expressive homology to the toxins Rbp (Shiga-like toxin) and TlyA (hemolysin) toxins. Phenotypic assays verified the cytotoxic effects of these proteins on different C. diphtheriae strains. In conclusion, C. diphtheriae strains isolated from cases of diphtheria and/or invasive infections may also utilize the production of TlyA hemolysin and Shiga-like toxin in the strain-dependent mechanisms of pathogenicity. Additional studies related to these virulence factors remain ongoing for both C. diphtheriae and other clinically relevant corynebacteriaAs manifestações clínicas observadas nas infecções por Corynebacterium diphtheriae, principal agente etiológico da difteria, são frequentemente associadas à ação da toxina diftérica (TD), seu principal fator de virulência. No entanto, cepas não produtoras de TD têm sido isoladas de infecções invasivas e estudos recentes vem demonstrando o envolvimento de mecanismo multifatorial na sua patogenicidade. A análise do pangenoma desta espécie identificou a presença de 57 ilhas genômicas, indicando que a transferência horizontal de genes é uma estratégia frequentemente utilizada pelas corinebactérias para estabelecer diferenças nas interações patógeno-hospedeiro. Diversos trabalhos indicam que os genes codificadores da toxina Shiga são passíveis de transferência entre espécies. Em Corynebacterium ulcerans a proteína Rbp, similar à cadeia A da toxina Shiga de Escherichia coli (SLT-1), cuja função é impedir a síntese de ribossomos provocando morte celular, foi identificada. Adicionalmente, os genes codificadores de hemolisinas, outro importante fator de virulência, são capazes de causar lesão celular através da formação de poros. Os genes da alfa-hemolisina (HlyA) de E. coli e, de outras espécies bacterianas, também são passíveis de transferência horizontal. Algumas cepas de C. diphtheriae parecem ser produtoras de hemolisinas específicas, uma vez que são classificadas quanto à atividade hemolítica. O sequenciamento genômico de C. diphtheriae biovar gravis cepa NCTC 13129 identificou a sequência de uma proteína hipotética, semelhante a hemolisina (TlyA) de Mycobacterium tuberculosis. Neste sentido, foi demonstrada a presença e a expressão de genes semelhantes às toxinas Shiga-like e hemolisina, em diferentes espécies de Corynebacterium. Pesquisas in silico da presença dos genes rbp (toxina Shiga-like) e tlyA (hemolisina) nas espécies de Corynebacterium. foram realizados. Adicionalmente, os seguintes experimentos para a confirmação fenotípica foram desenvolvidos: i) construção de cepa mutante para os genes alvos; ii) padronização de PCR e RT-qPCR; iii) expressão gênica em PCR por tempo real; iv) ensaios de citotoxicidade em células Vero; v) influência de diferentes condições ambientais na expressão destes genes, e vi) potencial artritogênico em modelos animais (murinos). Os resultados obtidos através de análises de bioinformática demonstraram que diferentes espécies de Corynebacterium apresentavam genes que codificam proteínas com expressiva homologia para as toxinas Rbp e TlyA. Os dados dos ensaios fenotípicos verificaram os efeitos citotóxicos destas proteínas em diferentes cepas de C. diphtheriae analisadas. Em conclusão, as cepas de C. diphtheriae isoladas de casos de difteria e/ou infecções invasivas podem também utilizar a produção de hemolisina TlyA e de toxina Shiga-like nos mecanismos cepa-dependentes de patogenicidade. Estudos adicionais relacionados a esses fatores de virulência permanecem em andamento tanto para C. diphtheriae quanto para outras corinebactérias de relevância clínicaSubmitted by Felipe CB/A (felipebibliotecario@gmail.com) on 2024-01-17T19:09:32Z No. of bitstreams: 1 Tese - Andrezza do Espírito Santo Cucinelli - 2022 - Completa.pdf: 5623067 bytes, checksum: e17a979be165870c36d010fc22b53e58 (MD5)Made available in DSpace on 2024-01-17T19:09:32Z (GMT). No. of bitstreams: 1 Tese - Andrezza do Espírito Santo Cucinelli - 2022 - Completa.pdf: 5623067 bytes, checksum: e17a979be165870c36d010fc22b53e58 (MD5) Previous issue date: 2022-06-29application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Ciências MédicasUERJBrasilCentro Biomédico::Faculdade de Ciências MédicasHemolysinShiga-like toxinCorynebacterium diphtheriae – PatogenicidadeToxina Shiga-like – GenéticaHemolisina – GenéticaGene tlyAGene rbpCIENCIAS BIOLOGICAS::GENETICAToxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulênciaShiga-like toxin and TlyA hemolysin in Corynebacterium diphtheriae: identification, functional characterization and influence on virulence potentialinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALTese - Andrezza do Espírito Santo Cucinelli - 2022 - Completa.pdfTese - Andrezza do Espírito Santo Cucinelli - 2022 - Completa.pdfapplication/pdf5623067http://www.bdtd.uerj.br/bitstream/1/20943/2/Tese+-+Andrezza+do+Esp%C3%ADrito+Santo+Cucinelli+-+2022+-+Completa.pdfe17a979be165870c36d010fc22b53e58MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82123http://www.bdtd.uerj.br/bitstream/1/20943/1/license.txte5502652da718045d7fcd832b79fca29MD511/209432024-02-26 15:59:52.07oai:www.bdtd.uerj.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T18:59:52Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
dc.title.alternative.eng.fl_str_mv |
Shiga-like toxin and TlyA hemolysin in Corynebacterium diphtheriae: identification, functional characterization and influence on virulence potential |
title |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
spellingShingle |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência Cucinelli, Andrezza do Espírito Santo Hemolysin Shiga-like toxin Corynebacterium diphtheriae – Patogenicidade Toxina Shiga-like – Genética Hemolisina – Genética Gene tlyA Gene rbp CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
title_full |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
title_fullStr |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
title_full_unstemmed |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
title_sort |
Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência |
author |
Cucinelli, Andrezza do Espírito Santo |
author_facet |
Cucinelli, Andrezza do Espírito Santo andrezzacucinelli@yahoo.com.br |
author_role |
author |
author2 |
andrezzacucinelli@yahoo.com.br |
author2_role |
author |
dc.contributor.advisor1.fl_str_mv |
Guaraldi, Ana Luíza de Mattos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8091118564093203 |
dc.contributor.advisor-co1.fl_str_mv |
Saliba, Alessandra Mattos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6134920105834158 |
dc.contributor.referee1.fl_str_mv |
Ignacio, Ana Claudia de Paula Rosa |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7809403473011306 |
dc.contributor.referee2.fl_str_mv |
Sant'Anna, Louisy Sanches dos Santos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5999066009401057 |
dc.contributor.referee3.fl_str_mv |
Gomes, Débora Leandro Rama |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4860403157095790 |
dc.contributor.referee4.fl_str_mv |
Neves, Felipe Piedade Gonçalves |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/5289973921789746 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6918848605710038 |
dc.contributor.author.fl_str_mv |
Cucinelli, Andrezza do Espírito Santo andrezzacucinelli@yahoo.com.br |
contributor_str_mv |
Guaraldi, Ana Luíza de Mattos Saliba, Alessandra Mattos Ignacio, Ana Claudia de Paula Rosa Sant'Anna, Louisy Sanches dos Santos Gomes, Débora Leandro Rama Neves, Felipe Piedade Gonçalves |
dc.subject.eng.fl_str_mv |
Hemolysin Shiga-like toxin |
topic |
Hemolysin Shiga-like toxin Corynebacterium diphtheriae – Patogenicidade Toxina Shiga-like – Genética Hemolisina – Genética Gene tlyA Gene rbp CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.por.fl_str_mv |
Corynebacterium diphtheriae – Patogenicidade Toxina Shiga-like – Genética Hemolisina – Genética Gene tlyA Gene rbp |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA |
description |
The clinical features observed in cases of classic diphtheria caused by Corynebacterium diphtheriae are associated with diphtheria toxin production (DT), its main virulence factor. However, non-DT-producing strains have been isolated from invasive infections. Recent studies have demonstrated the involvement of a multifactorial virulence mechanism expressed by C. diphtheriae strains. The analysis of the pangenome of this species identified the presence of 57 genomic islands, indicating that horizontal gene transfer is a strategy frequently used by corynebacteria to establish differences in pathogen-host interactions. Several studies indicate that the genes encoding Shiga toxin are transferable between species. In Corynebacterium ulcerans, the Rbp protein, similar to the A chain of Escherichia coli Shiga toxin (SLT-1), whose function is to inhibit the synthesis of ribosomes causing cell death, was previously identified. Additionally, genes encoding hemolysins, another important virulence factor, may cause cell damage through pore formation. The alpha-hemolysin (HlyA) genes from E. coli and other bacterial species are also capable of horizontal transfer. Some C. diphtheriae strains possibly produce specific hemolysins, as they are classified according to hemolytic activity. Genomic sequencing of C. diphtheriae biotype gravis NCTC 13129 strain identified the sequence of a hypothetical hemolysin-like protein (TlyA) from Mycobacterium tuberculosis. Therefore, the presence and expression of genes similar to Shiga-like toxins and hemolysin were demonstrated in different Corynebacterium species. Additionally, the following experiments for the investigation of phenotypic expression were performed: i) construction of mutant strains for the target genes: ii) gene expression by real-time PCR; iii) Vero cell cytotoxicity assays; iv) influence of environmental conditions on the expressions of these genes; v) arthritogenic potential using an animal model (murine). Data obtained through bioinformatics analysis showed that different Corynebacterium species presented genes that encode proteins with expressive homology to the toxins Rbp (Shiga-like toxin) and TlyA (hemolysin) toxins. Phenotypic assays verified the cytotoxic effects of these proteins on different C. diphtheriae strains. In conclusion, C. diphtheriae strains isolated from cases of diphtheria and/or invasive infections may also utilize the production of TlyA hemolysin and Shiga-like toxin in the strain-dependent mechanisms of pathogenicity. Additional studies related to these virulence factors remain ongoing for both C. diphtheriae and other clinically relevant corynebacteria |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-06-29 |
dc.date.accessioned.fl_str_mv |
2024-01-17T19:09:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CUCINELLI, Andrezza do Espírito Santo. Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência. 2022. 171 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2022. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/20943 |
identifier_str_mv |
CUCINELLI, Andrezza do Espírito Santo. Toxina Shiga-like e hemolisina TlyA em cepas de Corynebacterium diphtheriae: identificação, caracterização funcional e influência no potencial de virulência. 2022. 171 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2022. |
url |
http://www.bdtd.uerj.br/handle/1/20943 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Médicas |
dc.publisher.initials.fl_str_mv |
UERJ |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Centro Biomédico::Faculdade de Ciências Médicas |
publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UERJ instname:Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
instname_str |
Universidade do Estado do Rio de Janeiro (UERJ) |
instacron_str |
UERJ |
institution |
UERJ |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UERJ |
collection |
Biblioteca Digital de Teses e Dissertações da UERJ |
bitstream.url.fl_str_mv |
http://www.bdtd.uerj.br/bitstream/1/20943/2/Tese+-+Andrezza+do+Esp%C3%ADrito+Santo+Cucinelli+-+2022+-+Completa.pdf http://www.bdtd.uerj.br/bitstream/1/20943/1/license.txt |
bitstream.checksum.fl_str_mv |
e17a979be165870c36d010fc22b53e58 e5502652da718045d7fcd832b79fca29 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ) |
repository.mail.fl_str_mv |
bdtd.suporte@uerj.br |
_version_ |
1792352394042933248 |