Efeito do movimento dentário nos níveis de metaloproteinases da matriz no fluido gengival de pacientes com periodontite controlada

• Autor(a) principal: Almeida, Rhita Cristina Cunha
• Data de Publicação: 2012
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14018
• topic: Matrix metalloproteinases. Tooth movement. Gingival crevicular fluid; Metaloproteinases da matriz; Movimentação dentária; Líquido do sulco gengival; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The aim of this study was to evaluate whether orthodontic tooth movement of periodontally compromised teeth would result in detectable changes in gingival crevicular fluid (GCF) volume and in the levels of matrix metalloproteinases (MMP) -1, -2, -3, -7, -8, -12 and -13 in the GCF. Ten controlled periodontitis subjects (8 females and 2 males, mean age of 46.2 ± 10.4 years) with flared incisors were submitted to orthodontic treatment. One dental arch was subjected to orthodontic movement, and teeth in the opposite arch were used as controls. GCF samples were collected from the lingual sites of two test and two control incisors one week before orthodontic activation (-7d), immediately after orthodontic activation, and after 1 h, 24 h, and 7, 14 and 21 days. Filter paper strips were used in combination with a Periotron for GCF volume measurements. All patients received hygiene instructions and a kit containing toothbrush, fluoride dentifrice and gluconate chlorexidine 0.12% mouthwash to be used during all experiment. Multiplexed bead immunoassay was used to measure MMPs in GCF. Data were analyzed using Friedman and Mann-Whitney statistical tests. There were no statistically significant differences over time in the GCF volume for any of the two groups. Regarding the MMPs levels, the only significant change found over time was in the levels of MMP-1 in the movement group (p<0.05). When the two groups were compared after activation, the only statistically significant difference found was in levels of MMP-12 24 h after activation (p<0.05). This findings suggested that the GCF volume could not be associated to orthodontic movement and that the orthodontic movement of periodontally compromised teeth did not result in significant changes in the GCF levels of MMPs.