Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/15039 |
Resumo: | p. 308-312 |
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Colombo, FábioValença Neto, Alberto de Aguiar PiresSousa, Ana Paula Cavalcanti deMarchionni, Antônio Márcio TeixeiraPinheiro, Antonio Luiz BarbosaReis, Silvia Regina de AlmeidaColombo, FábioValença Neto, Alberto de Aguiar PiresSousa, Ana Paula Cavalcanti deMarchionni, Antônio Márcio TeixeiraPinheiro, Antonio Luiz BarbosaReis, Silvia Regina de Almeida2014-06-02T12:40:53Z2014-06-02T12:40:53Z20130103-6440http://repositorio.ufba.br/ri/handle/ri/15039v. 24, n. 4p. 308-312The aim of the present investigation was to evaluate the angiogenesis on dorsal cutaneous wounds in a rodent model treated with λ660 nm laser light. New vessel formation is a multistep process involving vessel sprouting, endothelial cell migration, proliferation and tube formation. Although several in vivo studies have shown that laser phototherapy influences tissue repair, a fully understanding of angiogenesis mechanisms are not yet known. Twenty-four young adult male Wistar rats weighing between 200 and 250 g were used. Under general anesthesia, one excisional wound was created on the dorsum of each animal and they were randomly distributed into two groups: one control and one treated with laser (λ660 nm, 16 mW, 10 J/cm2). Each group was subdivided into three subgroups according to the animal death timing (2, 4 and 6 days). Laser irradiation started immediately after surgery and was repeated every other day during the experiment and marked with Sirius Red, specific for collagen, and immunomarked with anti-TGF-β and anti-von Willebrand factor. Marked sections underwent histological analysis by light microscopy and the mean area of the wound of each animal was calculated and analyzed by ANOVA and Tukey's test (α=0.05). Although at some death periods, collagen expression and number of blood vessels on irradiated animals were higher than in the control ones, no significant differences were found at any time in relation to TGF-β expression (p>0.05). It was concluded that laser treatment (λ660 nm) contributed to increase angiogenesis.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-05-20T13:38:23Z No. of bitstreams: 1 0103-6440-bdj-24-04-308.pdf: 647044 bytes, checksum: 99838fb690eaa9b647e34a6d68bb93fe (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-06-02T12:40:52Z (GMT) No. of bitstreams: 1 0103-6440-bdj-24-04-308.pdf: 647044 bytes, checksum: 99838fb690eaa9b647e34a6d68bb93fe (MD5)Made available in DSpace on 2014-06-02T12:40:53Z (GMT). No. of bitstreams: 1 0103-6440-bdj-24-04-308.pdf: 647044 bytes, checksum: 99838fb690eaa9b647e34a6d68bb93fe (MD5) Previous issue date: 2013http://dx.doi.org/10.1590/0103-6440201301867reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAngiogenesisTGF-βLaserRepairGranulation tissueMast cellsVasodilationEffect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent modelBrazilian Dental Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengORIGINAL0103-6440-bdj-24-04-308.pdf0103-6440-bdj-24-04-308.pdfapplication/pdf647044https://repositorio.ufba.br/bitstream/ri/15039/1/0103-6440-bdj-24-04-308.pdf99838fb690eaa9b647e34a6d68bb93feMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15039/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT0103-6440-bdj-24-04-308.pdf.txt0103-6440-bdj-24-04-308.pdf.txtExtracted texttext/plain23735https://repositorio.ufba.br/bitstream/ri/15039/3/0103-6440-bdj-24-04-308.pdf.txt33367ce7c26e6b2abf6c37c48ab1b4c2MD53ri/150392022-07-05 14:04:04.972oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:04Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| dc.title.alternative.pt_BR.fl_str_mv |
Brazilian Dental Journal |
| title |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| spellingShingle |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model Colombo, Fábio Angiogenesis TGF-β Laser Repair Granulation tissue Mast cells Vasodilation |
| title_short |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| title_full |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| title_fullStr |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| title_full_unstemmed |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| title_sort |
Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model |
| author |
Colombo, Fábio |
| author_facet |
Colombo, Fábio Valença Neto, Alberto de Aguiar Pires Sousa, Ana Paula Cavalcanti de Marchionni, Antônio Márcio Teixeira Pinheiro, Antonio Luiz Barbosa Reis, Silvia Regina de Almeida |
| author_role |
author |
| author2 |
Valença Neto, Alberto de Aguiar Pires Sousa, Ana Paula Cavalcanti de Marchionni, Antônio Márcio Teixeira Pinheiro, Antonio Luiz Barbosa Reis, Silvia Regina de Almeida |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Colombo, Fábio Valença Neto, Alberto de Aguiar Pires Sousa, Ana Paula Cavalcanti de Marchionni, Antônio Márcio Teixeira Pinheiro, Antonio Luiz Barbosa Reis, Silvia Regina de Almeida Colombo, Fábio Valença Neto, Alberto de Aguiar Pires Sousa, Ana Paula Cavalcanti de Marchionni, Antônio Márcio Teixeira Pinheiro, Antonio Luiz Barbosa Reis, Silvia Regina de Almeida |
| dc.subject.por.fl_str_mv |
Angiogenesis TGF-β Laser Repair Granulation tissue Mast cells Vasodilation |
| topic |
Angiogenesis TGF-β Laser Repair Granulation tissue Mast cells Vasodilation |
| description |
p. 308-312 |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013 |
| dc.date.accessioned.fl_str_mv |
2014-06-02T12:40:53Z |
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2014-06-02T12:40:53Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/15039 |
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0103-6440 |
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v. 24, n. 4 |
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0103-6440 v. 24, n. 4 |
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http://repositorio.ufba.br/ri/handle/ri/15039 |
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Brasil |
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http://dx.doi.org/10.1590/0103-6440201301867 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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