Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/15535 |
Resumo: | p. 1-15 |
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Moens, BrittaPannecouque, ChristopheLópez, GiovanniTalledo, MichaelGotuzzo, EduardoCunha, Antônio Ricardo KhouriBittencourt, Achilea Candida LisboaFarré, LourdesCastro, Bernardo GalvãoVandamme, Anne MiekeVan Weyenbergh, JohanMoens, BrittaPannecouque, ChristopheLópez, GiovanniTalledo, MichaelGotuzzo, EduardoCunha, Antônio Ricardo KhouriBittencourt, Achilea Candida LisboaFarré, LourdesCastro, Bernardo GalvãoVandamme, Anne MiekeVan Weyenbergh, Johan2014-08-11T15:38:05Z2014-08-11T15:38:05Z20121743-422Xhttp://repositorio.ufba.br/ri/handle/ri/15535v. 9, n. 171p. 1-15Background IFN-α contributes extensively to host immune response upon viral infection through antiviral, pro-apoptotic, antiproliferative and immunomodulatory activities. Although extensively documented in various types of human cancers and viral infections, controversy exists in the exact mechanism of action of IFN-α in human immunodeficiency virus type 1 (HIV-1) and human T-lymphotropic virus type 1 (HTLV-1) retroviral infections. Results IFN-α displayed strong anti-HIV-1 effects in HIV-1/HTLV-1 co-infected MT-4 cells in vitro, demonstrated by the dose-dependent inhibition of the HIV-1-induced cytopathic effect (IC50 = 83.5 IU/ml, p < 0.0001) and p24 levels in cell-free supernatant (IC50 = 1.2 IU/ml, p < 0.0001). In contrast, IFN-α treatment did not affect cell viability or HTLV-1 viral mRNA levels in HTLV-1 mono-infected cell lines, based on flow cytometry and nCounter analysis, respectively. However, we were able to confirm the previously described post-transcriptional inhibition of HTLV-1 p19 secretion by IFN-α in cell lines (p = 0.0045), and extend this finding to primary Adult T cell Leukemia patient samples (p = 0.031). In addition, through microarray and nCounter analysis, we performed the first genome-wide simultaneous quantification of complete human and retroviral transciptomes, demonstrating significant transcriptional activation of interferon-stimulated genes without concomitant decrease of HTLV-1 mRNA levels. Conclusions Taken together, our results indicate that both the absence of in vitro antiproliferative and pro-apoptotic activity as well as the modest post-transcriptional antiviral activity of IFN-α against HTLV-1, were not due to a cell-intrinsic defect in IFN-α signalisation, but rather represents a retrovirus-specific phenomenon, considering the strong HIV-1 inhibition in co-infected cells.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-07-16T11:52:51Z No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-08-11T15:38:05Z (GMT) No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5)Made available in DSpace on 2014-08-11T15:38:05Z (GMT). No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1186/1743-422X-9-171reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBARetrovirusIFN-αHIV-1HTLV-1IFN-α signalingAntiviral activitySimultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell linesVirology Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALAchiléa Bittencourt.pdfAchiléa Bittencourt.pdfapplication/pdf1561034https://repositorio.ufba.br/bitstream/ri/15535/1/Achil%c3%a9a%20Bittencourt.pdf891e9491b8c19acf3073a5c4c4d4eecfMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15535/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTAchiléa Bittencourt.pdf.txtAchiléa Bittencourt.pdf.txtExtracted texttext/plain64632https://repositorio.ufba.br/bitstream/ri/15535/3/Achil%c3%a9a%20Bittencourt.pdf.txt630098110d80450446d6b21d53ecd14bMD53ri/155352022-08-08 13:19:12.885oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:19:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| dc.title.alternative.pt_BR.fl_str_mv |
Virology Journal |
| title |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| spellingShingle |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines Moens, Britta Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity |
| title_short |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| title_full |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| title_fullStr |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| title_full_unstemmed |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| title_sort |
Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines |
| author |
Moens, Britta |
| author_facet |
Moens, Britta Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antônio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Farré, Lourdes Castro, Bernardo Galvão Vandamme, Anne Mieke Van Weyenbergh, Johan |
| author_role |
author |
| author2 |
Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antônio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Farré, Lourdes Castro, Bernardo Galvão Vandamme, Anne Mieke Van Weyenbergh, Johan |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moens, Britta Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antônio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Farré, Lourdes Castro, Bernardo Galvão Vandamme, Anne Mieke Van Weyenbergh, Johan Moens, Britta Pannecouque, Christophe López, Giovanni Talledo, Michael Gotuzzo, Eduardo Cunha, Antônio Ricardo Khouri Bittencourt, Achilea Candida Lisboa Farré, Lourdes Castro, Bernardo Galvão Vandamme, Anne Mieke Van Weyenbergh, Johan |
| dc.subject.por.fl_str_mv |
Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity |
| topic |
Retrovirus IFN-α HIV-1 HTLV-1 IFN-α signaling Antiviral activity |
| description |
p. 1-15 |
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2012 |
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2012 |
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2014-08-11T15:38:05Z |
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2014-08-11T15:38:05Z |
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1743-422X |
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v. 9, n. 171 |
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1743-422X v. 9, n. 171 |
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