Evaluation of factors that can modify platelet-rich plasma properties

Detalhes bibliográficos
Autor(a) principal: Andrade, Miguel Gustavo Setúbal
Data de Publicação: 2008
Outros Autores: Brandão, Claudio José de Freitas, Sá, Camila Neves, Bittencourt, Thereza Cristina Borio dos Santos Calmon de, Sadigursky, Moysés
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6468
Resumo: Acesso restrito: Texto completo. p. 5-12
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spelling Andrade, Miguel Gustavo SetúbalBrandão, Claudio José de FreitasSá, Camila NevesBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, MoysésAndrade, Miguel Gustavo SetúbalBrandão, Claudio José de FreitasSá, Camila NevesBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, Moysés2012-07-19T14:08:40Z20081079-2104http://www.repositorio.ufba.br/ri/handle/ri/6468v. 105, n. 1Acesso restrito: Texto completo. p. 5-12Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time. Study design Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were obtained through automatic measurement. The amount of erythrocyte- and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson’s correlation coefficient. The significance level was set at P .05. A linear regression was performed to investigate the relationship among the correlated variables. Results From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/ L. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented significant Pearson correlation. Conclusions The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Wholeblood hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count is dependent upon whole-blood platelet count. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:e5-e12)Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-07-19T14:08:40Z No. of bitstreams: 1 __ac.els-cdn.com_S107921...b06ccf86387e5f6ee7e1ec7deba58.pdf: 223555 bytes, checksum: c35b2852f6742ca79ca7f90f95a11c18 (MD5)Made available in DSpace on 2012-07-19T14:08:40Z (GMT). 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dc.title.pt_BR.fl_str_mv Evaluation of factors that can modify platelet-rich plasma properties
dc.title.alternative.pt_BR.fl_str_mv ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
title Evaluation of factors that can modify platelet-rich plasma properties
spellingShingle Evaluation of factors that can modify platelet-rich plasma properties
Andrade, Miguel Gustavo Setúbal
title_short Evaluation of factors that can modify platelet-rich plasma properties
title_full Evaluation of factors that can modify platelet-rich plasma properties
title_fullStr Evaluation of factors that can modify platelet-rich plasma properties
title_full_unstemmed Evaluation of factors that can modify platelet-rich plasma properties
title_sort Evaluation of factors that can modify platelet-rich plasma properties
author Andrade, Miguel Gustavo Setúbal
author_facet Andrade, Miguel Gustavo Setúbal
Brandão, Claudio José de Freitas
Sá, Camila Neves
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
author_role author
author2 Brandão, Claudio José de Freitas
Sá, Camila Neves
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Miguel Gustavo Setúbal
Brandão, Claudio José de Freitas
Sá, Camila Neves
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
Andrade, Miguel Gustavo Setúbal
Brandão, Claudio José de Freitas
Sá, Camila Neves
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
description Acesso restrito: Texto completo. p. 5-12
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-07-19T14:08:40Z
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6468
dc.identifier.issn.none.fl_str_mv 1079-2104
dc.identifier.number.pt_BR.fl_str_mv v. 105, n. 1
identifier_str_mv 1079-2104
v. 105, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/6468
dc.language.iso.fl_str_mv eng
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