Evaluation of factors that can modify platelet-rich plasma properties
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/6468 |
Resumo: | Acesso restrito: Texto completo. p. 5-12 |
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Andrade, Miguel Gustavo SetúbalBrandão, Claudio José de FreitasSá, Camila NevesBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, MoysésAndrade, Miguel Gustavo SetúbalBrandão, Claudio José de FreitasSá, Camila NevesBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, Moysés2012-07-19T14:08:40Z20081079-2104http://www.repositorio.ufba.br/ri/handle/ri/6468v. 105, n. 1Acesso restrito: Texto completo. p. 5-12Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time. Study design Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were obtained through automatic measurement. The amount of erythrocyte- and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson’s correlation coefficient. The significance level was set at P .05. A linear regression was performed to investigate the relationship among the correlated variables. Results From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/ L. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented significant Pearson correlation. Conclusions The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Wholeblood hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count is dependent upon whole-blood platelet count. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:e5-e12)Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-07-19T14:08:40Z No. of bitstreams: 1 __ac.els-cdn.com_S107921...b06ccf86387e5f6ee7e1ec7deba58.pdf: 223555 bytes, checksum: c35b2852f6742ca79ca7f90f95a11c18 (MD5)Made available in DSpace on 2012-07-19T14:08:40Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Evaluation of factors that can modify platelet-rich plasma properties |
dc.title.alternative.pt_BR.fl_str_mv |
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY |
title |
Evaluation of factors that can modify platelet-rich plasma properties |
spellingShingle |
Evaluation of factors that can modify platelet-rich plasma properties Andrade, Miguel Gustavo Setúbal |
title_short |
Evaluation of factors that can modify platelet-rich plasma properties |
title_full |
Evaluation of factors that can modify platelet-rich plasma properties |
title_fullStr |
Evaluation of factors that can modify platelet-rich plasma properties |
title_full_unstemmed |
Evaluation of factors that can modify platelet-rich plasma properties |
title_sort |
Evaluation of factors that can modify platelet-rich plasma properties |
author |
Andrade, Miguel Gustavo Setúbal |
author_facet |
Andrade, Miguel Gustavo Setúbal Brandão, Claudio José de Freitas Sá, Camila Neves Bittencourt, Thereza Cristina Borio dos Santos Calmon de Sadigursky, Moysés |
author_role |
author |
author2 |
Brandão, Claudio José de Freitas Sá, Camila Neves Bittencourt, Thereza Cristina Borio dos Santos Calmon de Sadigursky, Moysés |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Miguel Gustavo Setúbal Brandão, Claudio José de Freitas Sá, Camila Neves Bittencourt, Thereza Cristina Borio dos Santos Calmon de Sadigursky, Moysés Andrade, Miguel Gustavo Setúbal Brandão, Claudio José de Freitas Sá, Camila Neves Bittencourt, Thereza Cristina Borio dos Santos Calmon de Sadigursky, Moysés |
description |
Acesso restrito: Texto completo. p. 5-12 |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2012-07-19T14:08:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/6468 |
dc.identifier.issn.none.fl_str_mv |
1079-2104 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 105, n. 1 |
identifier_str_mv |
1079-2104 v. 105, n. 1 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/6468 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1016/j.tripleo.2007.07.032 |
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UFBA |
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Repositório Institucional da UFBA |
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