Naïve donor responses to Schistosoma mansoni soluble egg antigens

Detalhes bibliográficos
Autor(a) principal: Reis, E. A. G.
Data de Publicação: 2007
Outros Autores: Azevedo, Theomira Mauadi Carmo, McBride, A. J. A., Harn Jr, D. A., Reis, M. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14486
Resumo: Texto completo: acesso restrito. p. 662-670
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spelling Reis, E. A. G.Azevedo, Theomira Mauadi CarmoMcBride, A. J. A.Harn Jr, D. A.Reis, M. G.Reis, E. A. G.Azevedo, Theomira Mauadi CarmoMcBride, A. J. A.Harn Jr, D. A.Reis, M. G.2014-01-28T13:20:34Z20070300-9475http://repositorio.ufba.br/ri/handle/ri/14486v. 66, n. 6Texto completo: acesso restrito. p. 662-670Schistosome infection induces profound Th-biasing and immune suppression. Although much has been examined in mice, few studies have examined responses of naïve humans to schistosome antigens. In this study, we examined the response of naïve human peripheral blood mononuclear cells (nPBMC) to stimulation with Schistosoma mansoni soluble egg antigen (SEA) using a priming in vitro (PIV) assay. We found that SEA induced a pronounced CD4+ T-helper cell response based on cytokine secretion and phenotyping markers. SEA-stimulated nPBMC (SEA cells) at day 7 post-priming and after the first recall consisted predominantly of Th0-like CD4+ T cells. Following the second recall, the majority of donor (10/12) responses were Th2-like. The cell population consisted of approximately 64% CD4+, 17% CD8+high, 12% CD19+, and 7% CD23+ cells. The CD4+ population also expressed HLA-DR+, CD54+, CD45RO+ and CD25+ whereas the CD19+ cells expressed CD80 and CD86. Following priming, we detected high levels of IL-6, IFN-γ, IL-12p40, IL-10 and IL-5. Upon restimulation, SEA cells secreted IL-5 and high levels of IL-10, typical of a Th2-like response. The data presented herein shows that the majority of naïve donor dendritic cells, following stimulation with SEA, prime and clonally expand SEA-specific T cells towards a Th2-type response. However, two donors responded with an atypical response, producing IFN-γ coincident with low levels of IL-10. Whether this differential response was due to HLA or other genes was not determined but is currently under investigation.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-06T13:25:16Z No. of bitstreams: 1 j.1365-3083.2007.02024.x.pdf: 430957 bytes, checksum: 195dd9ec4cd69f51ee6f6008a1a4fa5a (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-01-28T13:20:33Z (GMT) No. of bitstreams: 1 j.1365-3083.2007.02024.x.pdf: 430957 bytes, checksum: 195dd9ec4cd69f51ee6f6008a1a4fa5a (MD5)Made available in DSpace on 2014-01-28T13:20:34Z (GMT). No. of bitstreams: 1 j.1365-3083.2007.02024.x.pdf: 430957 bytes, checksum: 195dd9ec4cd69f51ee6f6008a1a4fa5a (MD5) Previous issue date: 2007http://dx.doi.org/10.1111/j.1365-3083.2007.02024.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASchistosoma mansoniCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesInterleukin-5Interleukin-10Th2 CellsHLA AntigensNaïve donor responses to Schistosoma mansoni soluble egg antigensScandinavian Journal of Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALj.1365-3083.2007.02024.x.pdfj.1365-3083.2007.02024.x.pdfapplication/pdf430957https://repositorio.ufba.br/bitstream/ri/14486/1/j.1365-3083.2007.02024.x.pdf195dd9ec4cd69f51ee6f6008a1a4fa5aMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14486/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTj.1365-3083.2007.02024.x.pdf.txtj.1365-3083.2007.02024.x.pdf.txtExtracted texttext/plain39370https://repositorio.ufba.br/bitstream/ri/14486/3/j.1365-3083.2007.02024.x.pdf.txtd4a66053931f4f0c339d4befcf87dbf5MD53ri/144862022-08-08 13:19:57.89oai:repositorio.ufba.br:ri/14486VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBLgoKIFBlbG8gcHJvY2Vzc28gZGUgc3VibWlzc++/vW8gZGUgZG9jdW1lbnRvcywgbyBhdXRvciBvdSBzZXUgcmVwcmVzZW50YW50ZSBsZWdhbCwgYW8gYWNlaXRhciAKZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEsIGNvbmNlZGUgYW8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRhIEJhaGlhIApvIGRpcmVpdG8gZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2Ye+/ve+/vW8uIApFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIGF1dG9yL2NvcHlyaWdodCwgbWFzIGVudGVuZGUgbyBkb2N1bWVudG8gCmNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4KCiBQYXJhIG9zIGRvY3VtZW50b3MgcHVibGljYWRvcyBjb20gcmVwYXNzZSBkZSBkaXJlaXRvcyBkZSBkaXN0cmlidWnvv73vv71vLCBlc3NlIHRlcm1vIGRlIGxpY2Vu77+9YSAKZW50ZW5kZSBxdWU6CgogTWFudGVuZG8gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHJlcGFzc2Fkb3MgYSB0ZXJjZWlyb3MsIGVtIGNhc28gZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8KcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIGludGVncmFsLCBtYXMgbGliZXJhIGFzIGluZm9ybWHvv73vv71lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gCmFzIHJlc3Ryae+/ve+/vWVzIGltcG9zdGFzIHBlbG9zIGVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2Hvv73vv71lcyBzZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVw77+9c2l0b3MgCmNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIG1hcyBtYW5077+9bSBhY2Vzc28gaXJyZXN0cml0byAKYW8gbWV0YWRhZG9zIGUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIGNvbnNlbnRpbWVudG8KIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgZXN0YXJlbSBlbSBpbmljaWF0aXZhcyBkZSBhY2Vzc28gYWJlcnRvLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:19:57Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Naïve donor responses to Schistosoma mansoni soluble egg antigens
dc.title.alternative.pt_BR.fl_str_mv Scandinavian Journal of Immunology
title Naïve donor responses to Schistosoma mansoni soluble egg antigens
spellingShingle Naïve donor responses to Schistosoma mansoni soluble egg antigens
Reis, E. A. G.
Schistosoma mansoni
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Interleukin-5
Interleukin-10
Th2 Cells
HLA Antigens
title_short Naïve donor responses to Schistosoma mansoni soluble egg antigens
title_full Naïve donor responses to Schistosoma mansoni soluble egg antigens
title_fullStr Naïve donor responses to Schistosoma mansoni soluble egg antigens
title_full_unstemmed Naïve donor responses to Schistosoma mansoni soluble egg antigens
title_sort Naïve donor responses to Schistosoma mansoni soluble egg antigens
author Reis, E. A. G.
author_facet Reis, E. A. G.
Azevedo, Theomira Mauadi Carmo
McBride, A. J. A.
Harn Jr, D. A.
Reis, M. G.
author_role author
author2 Azevedo, Theomira Mauadi Carmo
McBride, A. J. A.
Harn Jr, D. A.
Reis, M. G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reis, E. A. G.
Azevedo, Theomira Mauadi Carmo
McBride, A. J. A.
Harn Jr, D. A.
Reis, M. G.
Reis, E. A. G.
Azevedo, Theomira Mauadi Carmo
McBride, A. J. A.
Harn Jr, D. A.
Reis, M. G.
dc.subject.por.fl_str_mv Schistosoma mansoni
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Interleukin-5
Interleukin-10
Th2 Cells
HLA Antigens
topic Schistosoma mansoni
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Interleukin-5
Interleukin-10
Th2 Cells
HLA Antigens
description Texto completo: acesso restrito. p. 662-670
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-01-28T13:20:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14486
dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.number.pt_BR.fl_str_mv v. 66, n. 6
identifier_str_mv 0300-9475
v. 66, n. 6
url http://repositorio.ufba.br/ri/handle/ri/14486
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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