Anti-neosporal IgG and IgE antibodies in Canine Neosporosis

Detalhes bibliográficos
Autor(a) principal: Jesus, E. E. V.
Data de Publicação: 2007
Outros Autores: Almeida, Maria Angela Ornelas de, Atta, Ajax Mercês
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14562
Resumo: Texto completo: acesso restrito. p. 387-392
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spelling Jesus, E. E. V.Almeida, Maria Angela Ornelas deAtta, Ajax MercêsJesus, E. E. V.Almeida, Maria Angela Ornelas deAtta, Ajax Mercês2014-02-10T17:31:50Z20071863-1959http://repositorio.ufba.br/ri/handle/ri/14562v. 54, n. 9-10Texto completo: acesso restrito. p. 387-392Neospora caninum infection provokes neurological disorders, recurrent abortion and death in dogs and cattle. Dogs are both intermediate and definitive host of N. caninum. Thus, the development of sensitive and specific immunoassays to diagnose canine neosporosis is essential to control this disease. This work investigated serum anti-neosporal IgG and IgE antibodies in 140 dogs represented by 30 healthy animals (group I), 11 dogs showing acute N. caninum infection (group II), 50 urban dogs with serological evidence of canine neosporosis in indirect fluorescent antibody test (IFAT) (group III) and 49 urban dogs without clinical and laboratory evidences of neosporosis (group IV). Enzyme-linked immunosorbent assay (ELISA) and western immunoblotting, both using a soluble N. caninum tachyzoite antigen (SNA), investigated these two isotypes of antibodies, while a Urea-ELISA measured the avidity of the IgG antibodies. Anti-Toxoplasma gondii IgG antibodies were also investigated in the animals. Anti-neosporal IgG was found in all animals from groups II and III, whereas 32.7% (16/49) of dogs from group IV were reactive. IgG antibodies of low avidity were demonstrated in dogs from group II (median 35.3%), while animals from groups III and IV had IgG antibodies of high avidity (medians of 61.5% and 61.7% respectively). IgE antibodies were found in four (13.3%) and five (16.6%) dogs from groups III and IV respectively. Dogs presenting acute infection (group II) or chronic infection (group III) had IgG antibodies to several neosporal antigens, mainly of 29–30 and 35 kDa, while 13 of 16 dogs from group IV recognized antigens from 14 to 170 kDa. Antibodies to T. gondii were detected in 36 of 50 (72%) sera from group III and 25 of 49 (51%) sera from group IV. We concluded that IgG-ELISA and Urea-ELISA with SNA may substitute for IFAT in both laboratory routine and epidemiological studies of canine neosporosis.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-07T14:59:43Z No. of bitstreams: 1 j.1863-2378.2007.01076.x.pdf: 189934 bytes, checksum: a96f8a710cbe281224c4efab3cbd7355 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-02-10T17:31:50Z (GMT) No. of bitstreams: 1 j.1863-2378.2007.01076.x.pdf: 189934 bytes, checksum: a96f8a710cbe281224c4efab3cbd7355 (MD5)Made available in DSpace on 2014-02-10T17:31:50Z (GMT). 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dc.title.pt_BR.fl_str_mv Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
dc.title.alternative.pt_BR.fl_str_mv Zoonoses and Public Health
title Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
spellingShingle Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
Jesus, E. E. V.
Neospora caninum
Toxoplasma gondii
Dog
Immune response
IgG
IgE
Antibody avidity
title_short Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
title_full Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
title_fullStr Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
title_full_unstemmed Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
title_sort Anti-neosporal IgG and IgE antibodies in Canine Neosporosis
author Jesus, E. E. V.
author_facet Jesus, E. E. V.
Almeida, Maria Angela Ornelas de
Atta, Ajax Mercês
author_role author
author2 Almeida, Maria Angela Ornelas de
Atta, Ajax Mercês
author2_role author
author
dc.contributor.author.fl_str_mv Jesus, E. E. V.
Almeida, Maria Angela Ornelas de
Atta, Ajax Mercês
Jesus, E. E. V.
Almeida, Maria Angela Ornelas de
Atta, Ajax Mercês
dc.subject.por.fl_str_mv Neospora caninum
Toxoplasma gondii
Dog
Immune response
IgG
IgE
Antibody avidity
topic Neospora caninum
Toxoplasma gondii
Dog
Immune response
IgG
IgE
Antibody avidity
description Texto completo: acesso restrito. p. 387-392
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-02-10T17:31:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14562
dc.identifier.issn.none.fl_str_mv 1863-1959
dc.identifier.number.pt_BR.fl_str_mv v. 54, n. 9-10
identifier_str_mv 1863-1959
v. 54, n. 9-10
url http://repositorio.ufba.br/ri/handle/ri/14562
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1111/j.1863-2378.2007.01076.x
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instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
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