Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals

Detalhes bibliográficos
Autor(a) principal: Neves, Neuza Maria Alcântara
Data de Publicação: 2004
Outros Autores: Santos, Ricardo Ribeiro dos, Amor, Ana Lúcia Moreno, Uemura, Haruki, Silva Neto, Samuel J., Eichinger, Daniel, Carvalho, Lain Carlos Pontes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7029
Resumo: p. 273–278
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spelling Neves, Neuza Maria AlcântaraSantos, Ricardo Ribeiro dosAmor, Ana Lúcia MorenoUemura, HarukiSilva Neto, Samuel J.Eichinger, DanielCarvalho, Lain Carlos Pontes deNeves, Neuza Maria AlcântaraSantos, Ricardo Ribeiro dosAmor, Ana Lúcia MorenoUemura, HarukiSilva Neto, Samuel J.Eichinger, DanielCarvalho, Lain Carlos Pontes de2012-10-23T14:19:51Z2004http://www.repositorio.ufba.br/ri/handle/ri/7029v. 37, n. 5p. 273–278Trypanosoma cruzi is an obligate intracellular protozoan parasite that actively penetrates into non-phagocytic mammalian cells. To accomplish this, the parasite relies on the binding of cell surface ligands. It is reported herein that the T. cruzi trans-sialidase (TS), which is exposed on the parasite surface, binds to mouse heart cells, and should therefore be further studied as a possible cell penetration-related ligand. In addition, as has been proposed elsewhere, the binding of T. cruzi to tissues may turn them into targets for parasite-specific immune reactions. Washed heart sections from T. cruzi-infected mice were subjected to immunoenzymatic staining with antisera against whole T. cruzi and with polyclonal or monoclonal antibodies against TS. The anti-TS antibodies stained both parasites and uninfected heart cells in the vicinity of T. cruzi nest remains/trypomastigotes. On the other hand, an anti-T. cruzi serum, which did not recognize TS, only stained the parasites. In addition, normal heart sections from uninfected nude mice were shown to react with both enzymatically active and inactive recombinant TS molecules, probably through their amino-terminal region, since a recombinant TS lacking this region failed to bind.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-10-23T14:19:51Z No. of bitstreams: 1 __ac.els-cdn.com_S088240...226c700c0e910395082e7788b8517.pdf: 320439 bytes, checksum: ddff06dbdb7aee3df77fcf7cc203e9fa (MD5)Made available in DSpace on 2012-10-23T14:19:51Z (GMT). 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dc.title.pt_BR.fl_str_mv Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
dc.title.alternative.pt_BR.fl_str_mv Microbial Pathogenesis
title Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
spellingShingle Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
Neves, Neuza Maria Alcântara
Trypanosoma cruzi
Trans-sialidase
Binding
Heart tissue
title_short Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
title_full Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
title_fullStr Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
title_full_unstemmed Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
title_sort Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
author Neves, Neuza Maria Alcântara
author_facet Neves, Neuza Maria Alcântara
Santos, Ricardo Ribeiro dos
Amor, Ana Lúcia Moreno
Uemura, Haruki
Silva Neto, Samuel J.
Eichinger, Daniel
Carvalho, Lain Carlos Pontes de
author_role author
author2 Santos, Ricardo Ribeiro dos
Amor, Ana Lúcia Moreno
Uemura, Haruki
Silva Neto, Samuel J.
Eichinger, Daniel
Carvalho, Lain Carlos Pontes de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Neves, Neuza Maria Alcântara
Santos, Ricardo Ribeiro dos
Amor, Ana Lúcia Moreno
Uemura, Haruki
Silva Neto, Samuel J.
Eichinger, Daniel
Carvalho, Lain Carlos Pontes de
Neves, Neuza Maria Alcântara
Santos, Ricardo Ribeiro dos
Amor, Ana Lúcia Moreno
Uemura, Haruki
Silva Neto, Samuel J.
Eichinger, Daniel
Carvalho, Lain Carlos Pontes de
dc.subject.por.fl_str_mv Trypanosoma cruzi
Trans-sialidase
Binding
Heart tissue
topic Trypanosoma cruzi
Trans-sialidase
Binding
Heart tissue
description p. 273–278
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2012-10-23T14:19:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7029
dc.identifier.number.pt_BR.fl_str_mv v. 37, n. 5
url http://www.repositorio.ufba.br/ri/handle/ri/7029
identifier_str_mv v. 37, n. 5
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.micpath.2004.03.003
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