Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/13259 |
Resumo: | Texto completo. Acesso restrito. p. 37–42 |
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Silva, Wanderson M.Seyffert, NúbiaSantos, Agenor V.Castro, Thiago Luiz de PaulaPacheco, Luis Gustavo CarvalhoSantos, Anderson Rodrigues dosCiprandi, AlessandraDorella, Fernanda AlvesAndrade, Hélida M.Barh, DebmalyaPimenta, Adriano Monteiro de CastroSilva, Artur Luiz da Costa daMiyoshi, AndersonAzevedo, Vasco Ariston de CarvalhoSilva, Wanderson M.Seyffert, NúbiaSantos, Agenor V.Castro, Thiago Luiz de PaulaPacheco, Luis Gustavo CarvalhoSantos, Anderson Rodrigues dosCiprandi, AlessandraDorella, Fernanda AlvesAndrade, Hélida M.Barh, DebmalyaPimenta, Adriano Monteiro de CastroSilva, Artur Luiz da Costa daMiyoshi, AndersonAzevedo, Vasco Ariston de Carvalho2013-10-15T16:59:11Z2013-10-15T16:59:11Z20130882-4010http://www.repositorio.ufba.br/ri/handle/ri/13259v. 61–62Texto completo. Acesso restrito. p. 37–42This study involves the comparison between the exoproteomes of two different strains of Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis in small ruminants. In a previous study, based on a gel-free system (TPP-LC/MSE), 70 exoproteins for the strain 1002 and 67 for the strain C231, totaling 93 different extracellular proteins for C. pseudotuberculosis, were identified. In the present work, we have used 2D gel electrophoresis to resolve the extracellular proteins of both strains, which were then digested with trypsin, analyzed by MALDI-TOF/TOF and identified with the software MASCOT®. A total of 45 extracellular proteins of C. pseudotuberculosis were identified by this approach. The comparative analysis between the strains 1002 and C231 identified 13 and 3 strain-specific proteins, respectively, 11 of which are novel. These newly identified proteins may play an important role in the physiology and virulence of C. pseudotuberculosis.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-10-14T20:05:18Z No. of bitstreams: 1 111s.pdf: 864253 bytes, checksum: b7acccb5829c07dfda325cb65a0c3502 (MD5)Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-10-15T16:59:11Z (GMT) No. of bitstreams: 1 111s.pdf: 864253 bytes, checksum: b7acccb5829c07dfda325cb65a0c3502 (MD5)Made available in DSpace on 2013-10-15T16:59:11Z (GMT). No. of bitstreams: 1 111s.pdf: 864253 bytes, checksum: b7acccb5829c07dfda325cb65a0c3502 (MD5) Previous issue date: 2013SalvadorMicrobial Pathogenesishttp://www.sciencedirect.com/science/article/pii/S0882401013000818reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACorynebacterium pseudotuberculosisProteomicsExtracellular proteinsTwo-dimensional electrophoresis (2-DE)Caseous lymphadenitisIdentification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteomeMicrobial Pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL111s.pdf111s.pdfapplication/pdf864253https://repositorio.ufba.br/bitstream/ri/13259/1/111s.pdfb7acccb5829c07dfda325cb65a0c3502MD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13259/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXT111s.pdf.txt111s.pdf.txtExtracted texttext/plain28676https://repositorio.ufba.br/bitstream/ri/13259/3/111s.pdf.txtf5c249def3416c57a40edbb336684092MD53ri/132592022-07-05 14:03:47.763oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:47Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| dc.title.alternative.pt_BR.fl_str_mv |
Microbial Pathogenesis |
| title |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| spellingShingle |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome Silva, Wanderson M. Corynebacterium pseudotuberculosis Proteomics Extracellular proteins Two-dimensional electrophoresis (2-DE) Caseous lymphadenitis |
| title_short |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| title_full |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| title_fullStr |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| title_full_unstemmed |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| title_sort |
Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome |
| author |
Silva, Wanderson M. |
| author_facet |
Silva, Wanderson M. Seyffert, Núbia Santos, Agenor V. Castro, Thiago Luiz de Paula Pacheco, Luis Gustavo Carvalho Santos, Anderson Rodrigues dos Ciprandi, Alessandra Dorella, Fernanda Alves Andrade, Hélida M. Barh, Debmalya Pimenta, Adriano Monteiro de Castro Silva, Artur Luiz da Costa da Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho |
| author_role |
author |
| author2 |
Seyffert, Núbia Santos, Agenor V. Castro, Thiago Luiz de Paula Pacheco, Luis Gustavo Carvalho Santos, Anderson Rodrigues dos Ciprandi, Alessandra Dorella, Fernanda Alves Andrade, Hélida M. Barh, Debmalya Pimenta, Adriano Monteiro de Castro Silva, Artur Luiz da Costa da Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Wanderson M. Seyffert, Núbia Santos, Agenor V. Castro, Thiago Luiz de Paula Pacheco, Luis Gustavo Carvalho Santos, Anderson Rodrigues dos Ciprandi, Alessandra Dorella, Fernanda Alves Andrade, Hélida M. Barh, Debmalya Pimenta, Adriano Monteiro de Castro Silva, Artur Luiz da Costa da Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho Silva, Wanderson M. Seyffert, Núbia Santos, Agenor V. Castro, Thiago Luiz de Paula Pacheco, Luis Gustavo Carvalho Santos, Anderson Rodrigues dos Ciprandi, Alessandra Dorella, Fernanda Alves Andrade, Hélida M. Barh, Debmalya Pimenta, Adriano Monteiro de Castro Silva, Artur Luiz da Costa da Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho |
| dc.subject.por.fl_str_mv |
Corynebacterium pseudotuberculosis Proteomics Extracellular proteins Two-dimensional electrophoresis (2-DE) Caseous lymphadenitis |
| topic |
Corynebacterium pseudotuberculosis Proteomics Extracellular proteins Two-dimensional electrophoresis (2-DE) Caseous lymphadenitis |
| description |
Texto completo. Acesso restrito. p. 37–42 |
| publishDate |
2013 |
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2013-10-15T16:59:11Z |
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2013-10-15T16:59:11Z |
| dc.date.issued.fl_str_mv |
2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/13259 |
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0882-4010 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 61–62 |
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0882-4010 v. 61–62 |
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http://www.repositorio.ufba.br/ri/handle/ri/13259 |
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Microbial Pathogenesis |
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Microbial Pathogenesis |
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http://www.sciencedirect.com/science/article/pii/S0882401013000818 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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