Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)

Detalhes bibliográficos
Autor(a) principal: Silva, Vilma Mota da
Data de Publicação: 2005
Outros Autores: Veloso, Márcia Cristina da Cunha, Oliveira, Aline S. de, Santos, Gislaine Vieira dos, Pereira, Pedro Afonso de Paula, Andrade, Jailson Bittencourt de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5039
Resumo: p.323–328
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spelling Silva, Vilma Mota daVeloso, Márcia Cristina da CunhaOliveira, Aline S. deSantos, Gislaine Vieira dosPereira, Pedro Afonso de PaulaAndrade, Jailson Bittencourt deSilva, Vilma Mota daVeloso, Márcia Cristina da CunhaOliveira, Aline S. deSantos, Gislaine Vieira dosPereira, Pedro Afonso de PaulaAndrade, Jailson Bittencourt de2012-01-06T11:26:46Z2012-01-06T11:26:46Z20050039-9140http://www.repositorio.ufba.br/ri/handle/ri/5039v. 68.p.323–328Brominated phenols 2- and 4-bromophenol (2-BP and 4-BP); 2,4- and 2,6-dibromophenol (2,4-DBP and 2,6-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) have been identified as key flavor compounds found in seafoods. Depending on their concentrations, they were responsible for marine or ocean flavor (shrimp/crab/fish/sea salt-like) or for phenolic/iodine/iodoform-like off-flavor. In this work a new analytical methodology was developed to determine, simultaneously, such bromophenols in fish meats, based on reversed-phased high-performance liquid chromatographic separation (RP-HPLC). The separation of bromophenols was made onto a Lichrospher 100 RP-18 column using water:acetonitrile gradient at a flow rate of 1.0mLmin−1, using absorbance detection at 286 nm, were the 2-BP, 4-BP, 2,4- and 2,6-DBP show significant absorbtivity values and at 297 nm for 2,4,6-TBP. They were separated in 20 min with a good chromatographic resolution (Rs) for the isomeric compounds: 2- and 4-BP, Rs = 1.23; 2,4- and 2,6-DBP, Rs = 1.63. The calibration curves were linear in the bromophenols concentration range of 200.0–1000 ngmL−1. Under optimized conditions, the detection limit of the HPLC method was 127 ngmL−1 for 2-BP; 179 ngmL−1 for 4-BP; 89.0 ngmL−1 for 2,4-DBP; 269 ngmL−1 for 2,6-DBP and 232 ngmL−1 for 2,4,6-TBP. This method has been applied in determination of bromophenols, isolated by combined steam distillation-solvent extraction with 2mL of pentane/diethyl ether (6:4), from Brazilian fishes samples, collected on the Atlantic coast of Bahia (13◦01_S and 38◦31_W), Brazil. The concentration range determined were 0.20 ng g−1 (2-BP) to 299 ng g−1 (2,4,6-TBP). The method proposed here is rapid and suitable for simultaneous quantification of simple bromophenols in fish meat. As long as we know, it is the first analytical methodology, using RP-HPLC/UV, which was developed to determine simple bromophenols in fish meat.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-06T11:26:46Z No. of bitstreams: 1 determination_of__simple_bromophenols.pdf: 213709 bytes, checksum: 5e0164f5255c92da901889c05bbc19b9 (MD5)Made available in DSpace on 2012-01-06T11:26:46Z (GMT). No. of bitstreams: 1 determination_of__simple_bromophenols.pdf: 213709 bytes, checksum: 5e0164f5255c92da901889c05bbc19b9 (MD5) Previous issue date: 2005BromophenolsRP-HPLC/UVFishSeafoodDetermination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)Talantainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALdetermination_of__simple_bromophenols.pdfdetermination_of__simple_bromophenols.pdfapplication/pdf213709https://repositorio.ufba.br/bitstream/ri/5039/1/determination_of__simple_bromophenols.pdf5e0164f5255c92da901889c05bbc19b9MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5039/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTdetermination_of__simple_bromophenols.pdf.txtdetermination_of__simple_bromophenols.pdf.txtExtracted texttext/plain24506https://repositorio.ufba.br/bitstream/ri/5039/3/determination_of__simple_bromophenols.pdf.txt3628a2415036eaac59faeb7f51f38d07MD53ri/50392022-07-05 14:03:14.987oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
dc.title.alternative.pt_BR.fl_str_mv Talanta
title Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
spellingShingle Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
Silva, Vilma Mota da
Bromophenols
RP-HPLC/UV
Fish
Seafood
title_short Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
title_full Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
title_fullStr Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
title_full_unstemmed Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
title_sort Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)
author Silva, Vilma Mota da
author_facet Silva, Vilma Mota da
Veloso, Márcia Cristina da Cunha
Oliveira, Aline S. de
Santos, Gislaine Vieira dos
Pereira, Pedro Afonso de Paula
Andrade, Jailson Bittencourt de
author_role author
author2 Veloso, Márcia Cristina da Cunha
Oliveira, Aline S. de
Santos, Gislaine Vieira dos
Pereira, Pedro Afonso de Paula
Andrade, Jailson Bittencourt de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Vilma Mota da
Veloso, Márcia Cristina da Cunha
Oliveira, Aline S. de
Santos, Gislaine Vieira dos
Pereira, Pedro Afonso de Paula
Andrade, Jailson Bittencourt de
Silva, Vilma Mota da
Veloso, Márcia Cristina da Cunha
Oliveira, Aline S. de
Santos, Gislaine Vieira dos
Pereira, Pedro Afonso de Paula
Andrade, Jailson Bittencourt de
dc.subject.por.fl_str_mv Bromophenols
RP-HPLC/UV
Fish
Seafood
topic Bromophenols
RP-HPLC/UV
Fish
Seafood
description p.323–328
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2012-01-06T11:26:46Z
dc.date.available.fl_str_mv 2012-01-06T11:26:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5039
dc.identifier.issn.none.fl_str_mv 0039-9140
dc.identifier.number.pt_BR.fl_str_mv v. 68.
identifier_str_mv 0039-9140
v. 68.
url http://www.repositorio.ufba.br/ri/handle/ri/5039
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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reponame_str Repositório Institucional da UFBA
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