Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment

Detalhes bibliográficos
Autor(a) principal: Oliveira, I. C.
Data de Publicação: 2012
Outros Autores: Almeida, Rogeria Comastri de Castro, Hofer, Ernesto, Almeida, Paulo Fernando de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/8008
Resumo: p. 1128-1136
id UFBA-2_f16552cc1b90b08dc427b4e635e067cc
oai_identifier_str oai:repositorio.ufba.br:ri/8008
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Oliveira, I. C.Almeida, Rogeria Comastri de CastroHofer, ErnestoAlmeida, Paulo Fernando deOliveira, I. C.Almeida, Rogeria Comastri de CastroHofer, ErnestoAlmeida, Paulo Fernando de2013-01-21T19:11:05Z2013-01-21T19:11:05Z20121517-8382http://www.repositorio.ufba.br/ri/handle/ri/8008p. 1128-1136A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-01-21T19:11:05Z No. of bitstreams: 1 Bacteriophage amplification assay ......pdf: 198889 bytes, checksum: 7596aa502f17606c1a7b8dc52cd0a421 (MD5)Made available in DSpace on 2013-01-21T19:11:05Z (GMT). No. of bitstreams: 1 Bacteriophage amplification assay ......pdf: 198889 bytes, checksum: 7596aa502f17606c1a7b8dc52cd0a421 (MD5) Previous issue date: 2012SalvadorBrazilian Journal of MicrobiologyListeriabacteriophage A511laser lightdetectionskimmed milkBacteriophage amplification assay for detection of listeria spp. using virucidal laser treatmentBrazilian Journal of Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALBacteriophage amplification assay ......pdfBacteriophage amplification assay ......pdfapplication/pdf198889https://repositorio.ufba.br/bitstream/ri/8008/1/Bacteriophage%20amplification%20%20assay%20......pdf7596aa502f17606c1a7b8dc52cd0a421MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/8008/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTBacteriophage amplification assay ......pdf.txtBacteriophage amplification assay ......pdf.txtExtracted texttext/plain33731https://repositorio.ufba.br/bitstream/ri/8008/3/Bacteriophage%20amplification%20%20assay%20......pdf.txtc25928090fd039ae2afa49d4eee1f62dMD53ri/80082022-07-05 14:03:03.881oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
dc.title.alternative.pt_BR.fl_str_mv Brazilian Journal of Microbiology
title Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
spellingShingle Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
Oliveira, I. C.
Listeria
bacteriophage A511
laser light
detection
skimmed milk
title_short Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
title_full Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
title_fullStr Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
title_full_unstemmed Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
title_sort Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
author Oliveira, I. C.
author_facet Oliveira, I. C.
Almeida, Rogeria Comastri de Castro
Hofer, Ernesto
Almeida, Paulo Fernando de
author_role author
author2 Almeida, Rogeria Comastri de Castro
Hofer, Ernesto
Almeida, Paulo Fernando de
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, I. C.
Almeida, Rogeria Comastri de Castro
Hofer, Ernesto
Almeida, Paulo Fernando de
Oliveira, I. C.
Almeida, Rogeria Comastri de Castro
Hofer, Ernesto
Almeida, Paulo Fernando de
dc.subject.por.fl_str_mv Listeria
bacteriophage A511
laser light
detection
skimmed milk
topic Listeria
bacteriophage A511
laser light
detection
skimmed milk
description p. 1128-1136
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-01-21T19:11:05Z
dc.date.available.fl_str_mv 2013-01-21T19:11:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/8008
dc.identifier.issn.none.fl_str_mv 1517-8382
identifier_str_mv 1517-8382
url http://www.repositorio.ufba.br/ri/handle/ri/8008
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/8008/1/Bacteriophage%20amplification%20%20assay%20......pdf
https://repositorio.ufba.br/bitstream/ri/8008/2/license.txt
https://repositorio.ufba.br/bitstream/ri/8008/3/Bacteriophage%20amplification%20%20assay%20......pdf.txt
bitstream.checksum.fl_str_mv 7596aa502f17606c1a7b8dc52cd0a421
1b89a9a0548218172d7c829f87a0eab9
c25928090fd039ae2afa49d4eee1f62d
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1793970081637597184

Registros relacionados