The addition of the salmon oil in the freezing of equine semen

• Autor(a) principal: Agostinho,Raphael Farruk do Amaral
• Data de Publicação: 2017
• Outros Autores: Andrade,Vitor Ayub Assaf, Caiado,Renato Pinheiro da Silva, Barreto,Marcus Antônio Pessanha, Caiado,José Renato Costa, Shimoda,Eduardo, Silva,José Frederico Straggiotti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Saúde e Produção Animal
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000400604
• topic: cryopreservation; equine spermatozoa; polyunsaturated fatty acids

SUMARY The aim of the present study was to verify the effect of salmon oil addition on cryopreservation of equine semen. The experiment consisted of two treatments. Treatment 1 (T1) (control diluent), BotuCrio® was used without addition of salmon oil and treatment 2 (T2) (experimental diluent) BotuCrio® plus (with) 2% salmon oil. Three ejaculates of four stallions were used, totalizing 12 collections (n=12). Overall motility and progressive motility were evaluated by the Hamilton Thorn Research (HTR) Ceros 10.8 program, as well as the plasma membrane functionality through the hyposmotic test. Both treatments did not present statistical differences in relation to motility (T1 25,2±1,7 a, T2 29,7±1,9 a) and progressive motility (T1 11,0±1,1 a, T2 14,1±1,3 a). With respect to the hyposmotic test, the treatment 2 plus 2% of Salmon oil, presented better protection of sperm membrane functionality in relation to the control treatment (T2 77,3±1,0 a, T1 68,0±1,0 b). It can be concluded that salmon oil, although not altering the total and progressive motility, confers a better efficiency of sperm membrane functionality after thawing in equine semen.