Expression of NCED gene in colored cotton genotypes subjected to water stress
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Engenharia Agrícola e Ambiental (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800692 |
Resumo: | ABSTRACT Considering that the NCED gene acts on the biosynthetic cascade of ABA, a hormone involved in the functioning of stomata and consequently in the regulation of transpiration, the aim of this research was to analyze the expression of this gene in colored cotton genotypes subjected to water stress at the beginning of plant growth. Four colored cotton genotypes were used, subjected to two managements, with and without water stress, beginning the treatments when the blade of the first true leaves reached an area that allowed the evaluation of gas exchange. For the studies of the expression of the NCED gene, via RT-qPCR, leaves were collected on three distinct dates: at 4 and 6 days of water stress, and after the plants regained their turgor. The differential expression of NCED was found in all genotypes, with higher levels of expression related to six days of water stress. When the stomatal conductance was around 25%, there was overexpression in the genotype CNPA 2009.13, followed by CNPA 2009.6, BRS SAFIRA and CNPA 2009.11, confirming the data obtained in the semi-quantitative RT-PCR. The NCED gene is involved in the response to water stress in the vegetative phase of colored cotton. |
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Expression of NCED gene in colored cotton genotypes subjected to water stressGossypium hirsutum L.gas exchangesemi-quantitative RT-PCRqRT-PCRABSTRACT Considering that the NCED gene acts on the biosynthetic cascade of ABA, a hormone involved in the functioning of stomata and consequently in the regulation of transpiration, the aim of this research was to analyze the expression of this gene in colored cotton genotypes subjected to water stress at the beginning of plant growth. Four colored cotton genotypes were used, subjected to two managements, with and without water stress, beginning the treatments when the blade of the first true leaves reached an area that allowed the evaluation of gas exchange. For the studies of the expression of the NCED gene, via RT-qPCR, leaves were collected on three distinct dates: at 4 and 6 days of water stress, and after the plants regained their turgor. The differential expression of NCED was found in all genotypes, with higher levels of expression related to six days of water stress. When the stomatal conductance was around 25%, there was overexpression in the genotype CNPA 2009.13, followed by CNPA 2009.6, BRS SAFIRA and CNPA 2009.11, confirming the data obtained in the semi-quantitative RT-PCR. The NCED gene is involved in the response to water stress in the vegetative phase of colored cotton.Departamento de Engenharia Agrícola - UFCG2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800692Revista Brasileira de Engenharia Agrícola e Ambiental v.20 n.8 2016reponame:Revista Brasileira de Engenharia Agrícola e Ambiental (Online)instname:Universidade Federal de Campina Grande (UFCG)instacron:UFCG10.1590/1807-1929/agriambi.v20n8p692-696info:eu-repo/semantics/openAccessSouza,Alexandre M. S. deBatista,Vandré G. L.Pinheiro,Morganna P. N.Suassuna,Janivan F.Lima,Liziane M. deFernandes,Pedro D.eng2016-08-11T00:00:00Zoai:scielo:S1415-43662016000800692Revistahttp://www.scielo.br/rbeaaPUBhttps://old.scielo.br/oai/scielo-oai.php||agriambi@agriambi.com.br1807-19291415-4366opendoar:2016-08-11T00:00Revista Brasileira de Engenharia Agrícola e Ambiental (Online) - Universidade Federal de Campina Grande (UFCG)false |
dc.title.none.fl_str_mv |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
title |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
spellingShingle |
Expression of NCED gene in colored cotton genotypes subjected to water stress Souza,Alexandre M. S. de Gossypium hirsutum L. gas exchange semi-quantitative RT-PCR qRT-PCR |
title_short |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
title_full |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
title_fullStr |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
title_full_unstemmed |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
title_sort |
Expression of NCED gene in colored cotton genotypes subjected to water stress |
author |
Souza,Alexandre M. S. de |
author_facet |
Souza,Alexandre M. S. de Batista,Vandré G. L. Pinheiro,Morganna P. N. Suassuna,Janivan F. Lima,Liziane M. de Fernandes,Pedro D. |
author_role |
author |
author2 |
Batista,Vandré G. L. Pinheiro,Morganna P. N. Suassuna,Janivan F. Lima,Liziane M. de Fernandes,Pedro D. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza,Alexandre M. S. de Batista,Vandré G. L. Pinheiro,Morganna P. N. Suassuna,Janivan F. Lima,Liziane M. de Fernandes,Pedro D. |
dc.subject.por.fl_str_mv |
Gossypium hirsutum L. gas exchange semi-quantitative RT-PCR qRT-PCR |
topic |
Gossypium hirsutum L. gas exchange semi-quantitative RT-PCR qRT-PCR |
description |
ABSTRACT Considering that the NCED gene acts on the biosynthetic cascade of ABA, a hormone involved in the functioning of stomata and consequently in the regulation of transpiration, the aim of this research was to analyze the expression of this gene in colored cotton genotypes subjected to water stress at the beginning of plant growth. Four colored cotton genotypes were used, subjected to two managements, with and without water stress, beginning the treatments when the blade of the first true leaves reached an area that allowed the evaluation of gas exchange. For the studies of the expression of the NCED gene, via RT-qPCR, leaves were collected on three distinct dates: at 4 and 6 days of water stress, and after the plants regained their turgor. The differential expression of NCED was found in all genotypes, with higher levels of expression related to six days of water stress. When the stomatal conductance was around 25%, there was overexpression in the genotype CNPA 2009.13, followed by CNPA 2009.6, BRS SAFIRA and CNPA 2009.11, confirming the data obtained in the semi-quantitative RT-PCR. The NCED gene is involved in the response to water stress in the vegetative phase of colored cotton. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800692 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000800692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-1929/agriambi.v20n8p692-696 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Departamento de Engenharia Agrícola - UFCG |
publisher.none.fl_str_mv |
Departamento de Engenharia Agrícola - UFCG |
dc.source.none.fl_str_mv |
Revista Brasileira de Engenharia Agrícola e Ambiental v.20 n.8 2016 reponame:Revista Brasileira de Engenharia Agrícola e Ambiental (Online) instname:Universidade Federal de Campina Grande (UFCG) instacron:UFCG |
instname_str |
Universidade Federal de Campina Grande (UFCG) |
instacron_str |
UFCG |
institution |
UFCG |
reponame_str |
Revista Brasileira de Engenharia Agrícola e Ambiental (Online) |
collection |
Revista Brasileira de Engenharia Agrícola e Ambiental (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Engenharia Agrícola e Ambiental (Online) - Universidade Federal de Campina Grande (UFCG) |
repository.mail.fl_str_mv |
||agriambi@agriambi.com.br |
_version_ |
1750297684668841984 |