RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO

• Autor(a) principal: Colombo, Ronan Carlos
• Data de Publicação: 2016
• Outros Autores: Favetta, Vanessa, Faria, Ricardo Tadeu de, Andrade, Felipe Aranha de, Melem, Vanderli Marino
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Caatinga
• Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/3989
• topic: Micropropagation; Culture medium; Amorphous silica.; Micropropagação. Meio de cultura. Sílica amorfa.

Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.