Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFERSA |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/350 |
Resumo: | This study was aimed at researching anti-Leptospira spp. and anti-Brucella spp. agglutinins in cattle slaughtered in public slaughterhouses in the State of Rio Grande do Norte, as well as confirm the presence of these agents through PCR in the ovaries and epididymides of the slaughtered animals. For such purpose, serum, and ovary or epididymides were collected from cattle at the public slaughterhouses of that state. Sampling was performed during normal slaughter line, without any interference as the order, sex, age, or origin of the slaughtered animals. The screening of animal reagent to leptospirosis was made through the Microscopic Agglutination Test (MAT). To search for brucellosis, animals were screened by the Buffered Acidified Antigen test (BAA) and as a confirmatory test 2-mercaptoethanol (2-ME) was used. To detect bacterial DNA in samples of ovary and epididymides the Polymerase Chain Reaction (PCR) was used. Of all the 306 animals evaluated, 189 (61.76%) were positive for the MAT, where the predominant serovar was Hardjo (24.9%). The second most frequent serovar was Wolffi (14.8%), followed by Butembo, Icterhaemorrragiae, and Hebdomadis who contributed with frequencies ranging from 6.9% to 2.6%. Lower frequencies were found for the serovars Australis (1.6%), Pomona (1.1%), Grippotyphosa (1.1%) and Patoc (1.1%). Canicola, Autumnalis, and Sentot had frequencies lower than 1%. Three animals were positive for the BAA test, however only one of these reacted positively to 2-ME. Not one of the samples was positive for Brucella spp. and Leptopira spp. DNA through the PCR technique. Given the above mentioned data it was concluded that the Leptospira spp. infection in cattle in the semiarid region of Rio Grande do Norte occurs at higher frequency with the serovar Hardjo during the dry season of the year. The Brucella spp. infection is present but has very low frequency, and no DNA from Leptospira spp. and Brucella spp. was detected in the ovary and epididymis samples of the cattle examined |
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Sakamoto, Sidnei Miyoshihttp://lattes.cnpq.br/6538549762348659Azevedo, Sergio Santos dehttp://lattes.cnpq.br/8096740977759756Barrêto Júnior, Raimundo Alveshttp://lattes.cnpq.br/0516971232838494http://lattes.cnpq.br/6407060652662026Araújo, Kênia Suênia Meira de2016-08-15T20:31:15Z2014-06-252012-08-31ARAÚJO, Kênia Suênia Meira de. Detection of antibody and search of Leptospira spp. and Brucella spp. in cattle slaughtered in state of Rio Grande do Norte. 2012. 85 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012.https://repositorio.ufersa.edu.br/handle/tede/350This study was aimed at researching anti-Leptospira spp. and anti-Brucella spp. agglutinins in cattle slaughtered in public slaughterhouses in the State of Rio Grande do Norte, as well as confirm the presence of these agents through PCR in the ovaries and epididymides of the slaughtered animals. For such purpose, serum, and ovary or epididymides were collected from cattle at the public slaughterhouses of that state. Sampling was performed during normal slaughter line, without any interference as the order, sex, age, or origin of the slaughtered animals. The screening of animal reagent to leptospirosis was made through the Microscopic Agglutination Test (MAT). To search for brucellosis, animals were screened by the Buffered Acidified Antigen test (BAA) and as a confirmatory test 2-mercaptoethanol (2-ME) was used. To detect bacterial DNA in samples of ovary and epididymides the Polymerase Chain Reaction (PCR) was used. Of all the 306 animals evaluated, 189 (61.76%) were positive for the MAT, where the predominant serovar was Hardjo (24.9%). The second most frequent serovar was Wolffi (14.8%), followed by Butembo, Icterhaemorrragiae, and Hebdomadis who contributed with frequencies ranging from 6.9% to 2.6%. Lower frequencies were found for the serovars Australis (1.6%), Pomona (1.1%), Grippotyphosa (1.1%) and Patoc (1.1%). Canicola, Autumnalis, and Sentot had frequencies lower than 1%. Three animals were positive for the BAA test, however only one of these reacted positively to 2-ME. Not one of the samples was positive for Brucella spp. and Leptopira spp. DNA through the PCR technique. Given the above mentioned data it was concluded that the Leptospira spp. infection in cattle in the semiarid region of Rio Grande do Norte occurs at higher frequency with the serovar Hardjo during the dry season of the year. The Brucella spp. infection is present but has very low frequency, and no DNA from Leptospira spp. and Brucella spp. was detected in the ovary and epididymis samples of the cattle examinedObjetivou-se pesquisar aglutininas anti-Leptospira spp. e anti-Brucella spp. em bovinos de abatedouros públicos no Estado do Rio Grande do Norte, bem como confirmar através da PCR, a presença desses agentes nos ovários e epidídimos desses animais. Para isso, foram colhidos durante a linha normal de abate, o sangue e ovário ou epidídimo, de 306 animais no período de dezembro de 2010 a agosto de 2011. Através da Reação de Soroaglutinação Microscópica (SAM) foram detectados 189 (61,76%) animais sororeagente para Leptospira spp., sendo o sorovar Hardjo (24,9%) o mais predominante, seguido do Wolffi, Butembo, Icterhaemorrragiae e Hebdomadis que contribuiram com frequências que variaram de 14,8% a 2,6%. Os sorovares Australis, Pomona, Grippotyphosa, Patoc, Canícola, Autumnalis e Sentot foram menos frequentes. Em análise em separado, o sorovar Hadjo apresentou maior ocorrência no período seco do que no chuvoso (p=0,031). Para a brucelose os animais foram triados através da prova do Antígeno Acidificado Tamponado (AAT) e confirmados pela prova do 2-mercaptoetanol (2-ME). Três animais foram positivos ao AAT, desses apenas um reagiu positivamente ao 2-ME. A PCR foi utilizada para detecção do DNA bacteriano nas amostras de ovário e epidídimo. Nenhuma amostra foi positiva na PCR para detecção de DNA da Brucella spp. e Leptospira spp. Diante do exposto conclui-se que a infecção por Leptospira spp. em bovinos ocorre no semiárido do Rio Grande do Norte com maior frequência para o sorovar Hardjo durante o período seco do ano. A brucelose foi detectada, mas com baixa frequência. Através da PCR não foi detectado DNA de Leptospira spp. e Brucella spp. nos ovários e epidídimos dos animaisapplication/pdfhttp://repositorio.ufersa.edu.br/retrieve/712/KeniaSMA_DISSERT.pdf.jpgporUniversidade Federal Rural do Semi-ÁridoPrograma de Pós-Graduação em Ciência AnimalUFERSABRCentro de Ciências Agrárias - CCACC-BY-SAinfo:eu-repo/semantics/openAccessInfectious diseasesLeptospira spp.Brucella spp.OvaryEpididymisPCRBrucella spp.Doenças infecciosasEpidídimoLeptospira spp.ovárioPCRCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADetecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do NorteDetection of antibody and search of Leptospira spp. and Brucella spp. in cattle slaughtered in state of Rio Grande do Norteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UFERSAinstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSATEXTKeniaSMA_DISSERT.pdf.txtKeniaSMA_DISSERT.pdf.txtExtracted texttext/plain168698https://repositorio.ufersa.edu.br//bitstream/tede/350/3/KeniaSMA_DISSERT.pdf.txt390854bd8677cc07f6171b9ed8fb878aMD53ORIGINALKeniaSMA_DISSERT.pdfapplication/pdf1150269https://repositorio.ufersa.edu.br//bitstream/tede/350/1/KeniaSMA_DISSERT.pdf54d277c76ed42d8d5e0470e502ceca2fMD51THUMBNAILKeniaSMA_DISSERT.pdf.jpgKeniaSMA_DISSERT.pdf.jpgGenerated Thumbnailimage/jpeg3787https://repositorio.ufersa.edu.br//bitstream/tede/350/2/KeniaSMA_DISSERT.pdf.jpga44ad7f850663e0a4dde7e3cad0027d6MD52tede/3502022-07-05 14:01:12.341oai:repositorio.ufersa.edu.br:tede/350Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufersa.edu.br/PUBhttp://bdtd.ufersa.edu.br/oai/requestdirecaosisbi@ufersa.edu.br|| direcaosisbi@ufersa.edu.bropendoar:2022-07-05T17:01:12Biblioteca Digital de Teses e Dissertações da UFERSA - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.por.fl_str_mv |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
dc.title.alternative.eng.fl_str_mv |
Detection of antibody and search of Leptospira spp. and Brucella spp. in cattle slaughtered in state of Rio Grande do Norte |
title |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
spellingShingle |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte Araújo, Kênia Suênia Meira de Infectious diseases Leptospira spp. Brucella spp. Ovary Epididymis PCR Brucella spp. Doenças infecciosas Epidídimo Leptospira spp. ovário PCR CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
title_full |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
title_fullStr |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
title_full_unstemmed |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
title_sort |
Detecção de anticorpos e pesquisa de DNA de Leptospira spp. e Brucella spp. em bovinos abatidos no Estado do Rio Grande do Norte |
author |
Araújo, Kênia Suênia Meira de |
author_facet |
Araújo, Kênia Suênia Meira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Sakamoto, Sidnei Miyoshi |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6538549762348659 |
dc.contributor.referee1.fl_str_mv |
Azevedo, Sergio Santos de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8096740977759756 |
dc.contributor.referee2.fl_str_mv |
Barrêto Júnior, Raimundo Alves |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0516971232838494 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6407060652662026 |
dc.contributor.author.fl_str_mv |
Araújo, Kênia Suênia Meira de |
contributor_str_mv |
Sakamoto, Sidnei Miyoshi Azevedo, Sergio Santos de Barrêto Júnior, Raimundo Alves |
dc.subject.eng.fl_str_mv |
Infectious diseases Leptospira spp. Brucella spp. Ovary Epididymis PCR |
topic |
Infectious diseases Leptospira spp. Brucella spp. Ovary Epididymis PCR Brucella spp. Doenças infecciosas Epidídimo Leptospira spp. ovário PCR CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.por.fl_str_mv |
Brucella spp. Doenças infecciosas Epidídimo Leptospira spp. ovário PCR |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study was aimed at researching anti-Leptospira spp. and anti-Brucella spp. agglutinins in cattle slaughtered in public slaughterhouses in the State of Rio Grande do Norte, as well as confirm the presence of these agents through PCR in the ovaries and epididymides of the slaughtered animals. For such purpose, serum, and ovary or epididymides were collected from cattle at the public slaughterhouses of that state. Sampling was performed during normal slaughter line, without any interference as the order, sex, age, or origin of the slaughtered animals. The screening of animal reagent to leptospirosis was made through the Microscopic Agglutination Test (MAT). To search for brucellosis, animals were screened by the Buffered Acidified Antigen test (BAA) and as a confirmatory test 2-mercaptoethanol (2-ME) was used. To detect bacterial DNA in samples of ovary and epididymides the Polymerase Chain Reaction (PCR) was used. Of all the 306 animals evaluated, 189 (61.76%) were positive for the MAT, where the predominant serovar was Hardjo (24.9%). The second most frequent serovar was Wolffi (14.8%), followed by Butembo, Icterhaemorrragiae, and Hebdomadis who contributed with frequencies ranging from 6.9% to 2.6%. Lower frequencies were found for the serovars Australis (1.6%), Pomona (1.1%), Grippotyphosa (1.1%) and Patoc (1.1%). Canicola, Autumnalis, and Sentot had frequencies lower than 1%. Three animals were positive for the BAA test, however only one of these reacted positively to 2-ME. Not one of the samples was positive for Brucella spp. and Leptopira spp. DNA through the PCR technique. Given the above mentioned data it was concluded that the Leptospira spp. infection in cattle in the semiarid region of Rio Grande do Norte occurs at higher frequency with the serovar Hardjo during the dry season of the year. The Brucella spp. infection is present but has very low frequency, and no DNA from Leptospira spp. and Brucella spp. was detected in the ovary and epididymis samples of the cattle examined |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-08-31 |
dc.date.available.fl_str_mv |
2014-06-25 |
dc.date.accessioned.fl_str_mv |
2016-08-15T20:31:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Kênia Suênia Meira de. Detection of antibody and search of Leptospira spp. and Brucella spp. in cattle slaughtered in state of Rio Grande do Norte. 2012. 85 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufersa.edu.br/handle/tede/350 |
identifier_str_mv |
ARAÚJO, Kênia Suênia Meira de. Detection of antibody and search of Leptospira spp. and Brucella spp. in cattle slaughtered in state of Rio Grande do Norte. 2012. 85 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012. |
url |
https://repositorio.ufersa.edu.br/handle/tede/350 |
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por |
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por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Universidade Federal Rural do Semi-Árido |
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Programa de Pós-Graduação em Ciência Animal |
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UFERSA |
dc.publisher.country.fl_str_mv |
BR |
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Centro de Ciências Agrárias - CCA |
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Universidade Federal Rural do Semi-Árido |
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UFERSA |
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UFERSA |
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Biblioteca Digital de Teses e Dissertações da UFERSA |
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