Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás

Detalhes bibliográficos
Autor(a) principal: Calaça, Karine Louise
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8248
Resumo: The current study was developed to investigate the presence of Escherichia coli and Salmonella sp., to determine the antimicrobial susceptibility profile and to detect virulence genes of Escherichia coli isolates. For the study, 50 establishments were selected in Goiânia and municipalities in the metropolitan region, in which 141 samples of excreta were collected, 141 of feed, and 141 drinkers' swabs of psittacine kept in cages for resale. Samples were submitted to conventional bacteriology and PCR and Escherichia coli was identified in 9.7% (41/423) of the samples, 12% (17/141) in excreta, 8.5% (12/141) in the feed and 8.5% (12/141) in the drinkers' swabs. In the determination of antimicrobial susceptibility of Escherichia coli samples isolated, was found resistance to ciprofloxacin 4.9% (2/41), gentamicin 17% (7/41), doxycycline 34.1% (14/41), florfenicol 34.1% (14/41), trimethoprim 39% (16/41), tetracycline 41.5% (17/41), enrofloxacin 43.9% (18/41), amoxycillin 48.8% (20/41 ), neomycin 61% (25/41) and sulfonamide 90.2% (37/41). The iss gene was detected in three isolates, tsh in three, papC in two and the traT and eae genes were not detected. Salmonella sp. was not isolated in the samples. It concludes that Escherichia coli isolates show resistance to the most commonly used antimicrobials and the possibility exists that these birds are carriers of pathogenic strains.
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spelling Andrade, Maria Auxiliadorahttp://lattes.cnpq.br/9441751521255467Jayme, Valéria de Sáhttp://lattes.cnpq.br/0603234425928309Andrade, Maria AuxiliadoraSola, Marília CristinaPôrto, Regiani Nascimento Gagnohttp://lattes.cnpq.br/6407769576948369Calaça, Karine Louise2018-03-23T14:48:47Z2018-02-28CALAÇA, K. L. Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás. 2018. 44 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/8248The current study was developed to investigate the presence of Escherichia coli and Salmonella sp., to determine the antimicrobial susceptibility profile and to detect virulence genes of Escherichia coli isolates. For the study, 50 establishments were selected in Goiânia and municipalities in the metropolitan region, in which 141 samples of excreta were collected, 141 of feed, and 141 drinkers' swabs of psittacine kept in cages for resale. Samples were submitted to conventional bacteriology and PCR and Escherichia coli was identified in 9.7% (41/423) of the samples, 12% (17/141) in excreta, 8.5% (12/141) in the feed and 8.5% (12/141) in the drinkers' swabs. In the determination of antimicrobial susceptibility of Escherichia coli samples isolated, was found resistance to ciprofloxacin 4.9% (2/41), gentamicin 17% (7/41), doxycycline 34.1% (14/41), florfenicol 34.1% (14/41), trimethoprim 39% (16/41), tetracycline 41.5% (17/41), enrofloxacin 43.9% (18/41), amoxycillin 48.8% (20/41 ), neomycin 61% (25/41) and sulfonamide 90.2% (37/41). The iss gene was detected in three isolates, tsh in three, papC in two and the traT and eae genes were not detected. Salmonella sp. was not isolated in the samples. It concludes that Escherichia coli isolates show resistance to the most commonly used antimicrobials and the possibility exists that these birds are carriers of pathogenic strains.O presente estudo foi desenvolvido no intuito de investigar a presença de Escherichia coli e Salmonella sp., determinar o perfil de sensibilidade aos antimicrobianos e detectar genes de virulência dos isolados de Escherichia coli. Para o estudo foram selecionados, por conveniência, 50 estabelecimentos em Goiânia e municípios da região metropolitana, nos quais foram coletadas 141 amostras de excretas, 141 de ração nos comedouros, e 141 de suabes de bebedouro dos psitacídeos mantidos em gaiolas para revenda. As amostras foram submetidas a bacteriologia convencional e PCR onde Escherichia coli foi identificada em 9,7% (41/423) das amostras, sendo 12% (17/141) em excretas, 8,5% (12/141) na ração e 8,5% (12/141) nos suabes de bebedouro. Na determinação da suscetibilidade aos antimicrobianos das amostras de Escherichia coli isoladas, obteve-se resistência a ciprofloxacina em 4,9% (2/41), gentamicina 17% (7/41), doxiciclina 34,1% (14/41), florfenicol 34,1% (14/41), trimetropin 39% (16/41), tetraciclina 41,5% (17/41), enrofloxacina 43,9% (18/41), amoxicilina 48,8% (20/41), neomicina 61% (25/41) e sulfonamida 90,2% (37/41). O gene iss foi detectado em três isolados, o tsh em três, o papC em dois e os genes traT e eae não foram detectados. Não foi isolada Salmonella sp. nas amostras. Diante de tais resultados, conclui-se que os isolados de Escherichia coli apresentam resistência aos antimicrobianos mais utilizados e existe a possibilidade dessas aves serem carreadoras de cepas patogênicas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2018-03-23T13:47:11Z No. of bitstreams: 2 Dissertação - Karine Louise Calaça - 2018.pdf: 2457467 bytes, checksum: 69d21cfe5b1374658e1d19546996b14f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-03-23T14:48:47Z (GMT) No. of bitstreams: 2 Dissertação - Karine Louise Calaça - 2018.pdf: 2457467 bytes, checksum: 69d21cfe5b1374658e1d19546996b14f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-03-23T14:48:47Z (GMT). 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dc.title.eng.fl_str_mv Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
dc.title.alternative.eng.fl_str_mv Search for Salmonella sp. and Escherichia coli with determination of resistance profile in psittacines of resellers in the metropolitan region of Goiânia - Goiás
title Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
spellingShingle Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
Calaça, Karine Louise
Aves silvestres
Colibacilose
PCR
Salmonelose
Colibacillosis
PCR
Salmonellosis
Wild birds
CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
title_short Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
title_full Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
title_fullStr Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
title_full_unstemmed Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
title_sort Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás
author Calaça, Karine Louise
author_facet Calaça, Karine Louise
author_role author
dc.contributor.advisor1.fl_str_mv Andrade, Maria Auxiliadora
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9441751521255467
dc.contributor.advisor-co1.fl_str_mv Jayme, Valéria de Sá
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/0603234425928309
dc.contributor.referee1.fl_str_mv Andrade, Maria Auxiliadora
dc.contributor.referee2.fl_str_mv Sola, Marília Cristina
dc.contributor.referee3.fl_str_mv Pôrto, Regiani Nascimento Gagno
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6407769576948369
dc.contributor.author.fl_str_mv Calaça, Karine Louise
contributor_str_mv Andrade, Maria Auxiliadora
Jayme, Valéria de Sá
Andrade, Maria Auxiliadora
Sola, Marília Cristina
Pôrto, Regiani Nascimento Gagno
dc.subject.por.fl_str_mv Aves silvestres
Colibacilose
PCR
Salmonelose
topic Aves silvestres
Colibacilose
PCR
Salmonelose
Colibacillosis
PCR
Salmonellosis
Wild birds
CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
dc.subject.eng.fl_str_mv Colibacillosis
PCR
Salmonellosis
Wild birds
dc.subject.cnpq.fl_str_mv CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
description The current study was developed to investigate the presence of Escherichia coli and Salmonella sp., to determine the antimicrobial susceptibility profile and to detect virulence genes of Escherichia coli isolates. For the study, 50 establishments were selected in Goiânia and municipalities in the metropolitan region, in which 141 samples of excreta were collected, 141 of feed, and 141 drinkers' swabs of psittacine kept in cages for resale. Samples were submitted to conventional bacteriology and PCR and Escherichia coli was identified in 9.7% (41/423) of the samples, 12% (17/141) in excreta, 8.5% (12/141) in the feed and 8.5% (12/141) in the drinkers' swabs. In the determination of antimicrobial susceptibility of Escherichia coli samples isolated, was found resistance to ciprofloxacin 4.9% (2/41), gentamicin 17% (7/41), doxycycline 34.1% (14/41), florfenicol 34.1% (14/41), trimethoprim 39% (16/41), tetracycline 41.5% (17/41), enrofloxacin 43.9% (18/41), amoxycillin 48.8% (20/41 ), neomycin 61% (25/41) and sulfonamide 90.2% (37/41). The iss gene was detected in three isolates, tsh in three, papC in two and the traT and eae genes were not detected. Salmonella sp. was not isolated in the samples. It concludes that Escherichia coli isolates show resistance to the most commonly used antimicrobials and the possibility exists that these birds are carriers of pathogenic strains.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-03-23T14:48:47Z
dc.date.issued.fl_str_mv 2018-02-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv CALAÇA, K. L. Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás. 2018. 44 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/8248
identifier_str_mv CALAÇA, K. L. Pesquisa de Salmonella sp. e Escherichia coli com determinação do perfil de resistência em psitacídeos de revendas na região metropolitana de Goiânia - Goiás. 2018. 44 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.
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dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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