Manipulações alimentares em diferentes fases do desenvolvimento de bovinos
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/9318 |
Resumo: | The objective of this study was to evaluate the physiological, biochemical and metabolic effects of milk restriction and prebiotic supplementation in suckling calves, and the same effects of supplementation of taurine in the bovine termination phase. Two experiments were executed to achieve the mentioned objectives. In the first trial it was used 20 crossbred calves with approximate initial weight of 38 kg and housed in individual pens for 56 days divided into two periods of 28 days each. The animals were allocaded in four experimental groups: animals receiving 6 L of milk in periods 1 and 2 (CON), animals with 50% milk restriction in the first period and re-alimented in the second period (RES), animals without restriction and supplemented of 5 g/day of mannanoligosaccharide (MOS) or mannan- frutoligosaccharide (MFOS). The weight gain and feed intake were verified. Serum was used to determinate glucose, lactate, creatinine, alkaline phosphatase, triglycerides, urea, toal protein and the hormones ghrelin and leptin. In the brain, in the paraventricular region of the hypothalamus, ghrelin receptor expression (GHS-R1a) was evaluated. The rumen and small intestine were used to evaluate the development of the gastrointestinal tract: rumen papilla length, villus height and intestinal crypt depth. We observed a lower weight gain of the restricted group in the first period and no difference in the weight gain among groups in the second period. It was observed that animals from the prebiotic supplemented (MOS) group showed a significant increase in jejunal villus height. Ruminal development was favored by the supplementation of mannanoligosaccharides (MOS group), which significantly increased ruminal papillae length. No difference was found for serum concentration of ghrelin and leptin among treatments in periods 1 and 2 (P> 0.05). Regarding the expression of ghrelin receptors in the paraventricular region of the hypothalamus, there was no difference between the groups evaluated. The second experiment was performed in 123 days with 80 finishing steers, mean initial weight of 507 kg and divided into groups supplemented with: 0; 0.025; 0.05; 0.075 and 0.1% taurine in the diet. Changes in performance were evaluated by weighing and control of the daily feed intake, carcass traits were evaluated after animals slaughter with data provided by the slaughter hose, and the metabolic changes were measured by plasma glucose, lactate, taurine and triglycerides values. Immediately after slaughter, a sample of Sternocephalicus ventrally muscle was collected to analyze the glycolytic potential. To complement the data from the second in vivo assay, an in vitro assay was performed using ruminal fluid cultures. Measurements included concentrations of VFA, pH, IVDMD and fermentative gas production for cultures containing 0; 0.025; 0.05; 0.075 and 0.1% taurine on the substrate. Supplementation of taurine did not affect the final characteristics of weight, HCW, DMI and carcass traits. Blood metabolites were not affected by administration of taurine. In addition, the production of in vitro gases, the concentrations of IVDMD and VFA’s were not affected by the addition of taurine. A linear response was detected for pH (P = 0.006) and with cattle consuming 0.1% taurine had the lowest pH. The compensatory gain presented by the restrictd milk animals, but did not accelerate the intestinal and ruminal development, while the supplementation of mananoligosaccharides caused an accelerated growth of the ruminal papillae and villi of the jejunum. Mannan-frutoligosaccharide supplementation did not accelerate the development of suckling calves. Taurine supplementation did not cause any change in the finishing phase of cattle, however, it caused a decrease in rumen pH. |
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Nassar, Reginaldo Ferreirahttp://lattes.cnpq.br/2555785079833283Pansani, Aline PatríciaAraujo Neto, Francisco Ribeiro deCastanheira, MarlosSantos, Fabiana Ramos doshttp://lattes.cnpq.br/6892272740607732Costa, Natália Alves2019-03-07T14:02:39Z2019-02-22COSTA, N. A. Manipulações alimentares em diferentes fases do desenvolvimento de bovinos. 2019. 120 f. Tese (Doutorado em Ciências Fisiológicas) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/9318The objective of this study was to evaluate the physiological, biochemical and metabolic effects of milk restriction and prebiotic supplementation in suckling calves, and the same effects of supplementation of taurine in the bovine termination phase. Two experiments were executed to achieve the mentioned objectives. In the first trial it was used 20 crossbred calves with approximate initial weight of 38 kg and housed in individual pens for 56 days divided into two periods of 28 days each. The animals were allocaded in four experimental groups: animals receiving 6 L of milk in periods 1 and 2 (CON), animals with 50% milk restriction in the first period and re-alimented in the second period (RES), animals without restriction and supplemented of 5 g/day of mannanoligosaccharide (MOS) or mannan- frutoligosaccharide (MFOS). The weight gain and feed intake were verified. Serum was used to determinate glucose, lactate, creatinine, alkaline phosphatase, triglycerides, urea, toal protein and the hormones ghrelin and leptin. In the brain, in the paraventricular region of the hypothalamus, ghrelin receptor expression (GHS-R1a) was evaluated. The rumen and small intestine were used to evaluate the development of the gastrointestinal tract: rumen papilla length, villus height and intestinal crypt depth. We observed a lower weight gain of the restricted group in the first period and no difference in the weight gain among groups in the second period. It was observed that animals from the prebiotic supplemented (MOS) group showed a significant increase in jejunal villus height. Ruminal development was favored by the supplementation of mannanoligosaccharides (MOS group), which significantly increased ruminal papillae length. No difference was found for serum concentration of ghrelin and leptin among treatments in periods 1 and 2 (P> 0.05). Regarding the expression of ghrelin receptors in the paraventricular region of the hypothalamus, there was no difference between the groups evaluated. The second experiment was performed in 123 days with 80 finishing steers, mean initial weight of 507 kg and divided into groups supplemented with: 0; 0.025; 0.05; 0.075 and 0.1% taurine in the diet. Changes in performance were evaluated by weighing and control of the daily feed intake, carcass traits were evaluated after animals slaughter with data provided by the slaughter hose, and the metabolic changes were measured by plasma glucose, lactate, taurine and triglycerides values. Immediately after slaughter, a sample of Sternocephalicus ventrally muscle was collected to analyze the glycolytic potential. To complement the data from the second in vivo assay, an in vitro assay was performed using ruminal fluid cultures. Measurements included concentrations of VFA, pH, IVDMD and fermentative gas production for cultures containing 0; 0.025; 0.05; 0.075 and 0.1% taurine on the substrate. Supplementation of taurine did not affect the final characteristics of weight, HCW, DMI and carcass traits. Blood metabolites were not affected by administration of taurine. In addition, the production of in vitro gases, the concentrations of IVDMD and VFA’s were not affected by the addition of taurine. A linear response was detected for pH (P = 0.006) and with cattle consuming 0.1% taurine had the lowest pH. The compensatory gain presented by the restrictd milk animals, but did not accelerate the intestinal and ruminal development, while the supplementation of mananoligosaccharides caused an accelerated growth of the ruminal papillae and villi of the jejunum. Mannan-frutoligosaccharide supplementation did not accelerate the development of suckling calves. Taurine supplementation did not cause any change in the finishing phase of cattle, however, it caused a decrease in rumen pH.Este trabalho teve como objetivo avaliar os efeitos fisiológicos, bioquímicos e metabólicos da restrição de leite e suplementação de prebióticos na fase de aleitamento e, os mesmos efeitos, da suplementação de taurina na fase de terminação de bovinos. Foram realizados dois experimentos para alcançar os objetivos mencionados. No primeiro ensaio foram utilizados 20 bezerros com peso inicial aproximado de 38 kg e alojados em baias individuais por 56 dias divididos em dois períodos de 28 dias cada. Os animais foram distribuídos em quatro grupos experimentais: animais que receberam 6 L de leite nos períodos 1 e 2 (CON), animais com restrição de 50% do leite no primeiro período e realimentados no segundo período (RES), animais sem restrição recebendo suplementação de 5 g/dia de mananoligossacarídeo (MOS) ou frutoligossacarídeo (MFOS) em animais sem restrição. Foi verificado o ganho de peso e consumo de ração. O soro foi utilizado para a determinação de glicose, lactato, creatinina, triglicerídeos, urea, proteína toal e dos hormônios grelina e leptina. Na região paraventricular do hipotálamo, foi avaliada a expressão do receptor de grelina (GHS-R1a). O rúmen e intestino delgado foram utilizados para avaliar o desenvolvimento do trato gastrointestinal: comprimento de papila ruminal, altura de vilosidade e profundidade de cripta intestinal. Foi observado um menor ganho de peso do grupo restrito no primeiro período e nenhuma diferença no ganho de peso dos grupos no segundo período. Observou-se que os animais do grupo suplementado com prebiótico (MOS) apresentaram um aumento significativo na altura da vilosidade jejunal. O desenvolvimento ruminal foi favorecido pela suplementação de mananoligossacarídeos (grupo MOS), que aumentou significativamente o comprimento das papilas ruminais. Nenhuma diferença foi encontrada entre os tratamentos nos períodos 1 e 2 (P > 0,05) entre os grupos para concentração sérica de grelina e leptina e na expressão de receptores de grelina na região paraventricular do hipotálamo. O segundo ensaio foi realizado por um período de 123 dias com 80 bovinos de corte em fase de terminação, peso inicial médio de 507 kg e divididos em grupos com suplementação de 0; 0,025; 0,05; 0,075 e 0,1% de taurina na dieta. A performance foi avaliada pelas pesagens e controle do alimento ingerido diariamente, características da carcaça foram avaliadas após o abate dos animais com dados fornecidos pelo frigorífico e as alterações metabólicas foram mensuradas pelos valores plasmáticos de glicose, lactato, taurina, triglicerídeos. Imediatamente após o abate foi coletado uma porção do músculo Sternocephalicus ventrally para análise do potencial glicolítico. Para complementar os dados do segundo ensaio in vivo, foi realizado um ensaio in vitro utilizando culturas de fluido ruminal. As medições incluíram concentrações de AGV, pH, DIVMS e produção de gás fermentativo para culturas contendo 0; 0,025; 0,05; 0,075 e 0,1% de taurina no substrato. A suplementação de taurina não afetou as características finais de peso, PCQ, CMS, parâmetros de carcaça, os metabólitos sanguíneos, produção de gases, DIVMS e AGV não foram afetados pela administração de taurina. Uma resposta linear foi detectada para o pH (P = 0,006), com bovinos consumindo 0,1% de taurina tiveram o pH mais baixo. O ganho compensatório apresentado pelos animais que passaram por restrição de leite, mas não acelerou o desenvolvimento intestinal e ruminal, enquanto que a suplementação de mananoligossacarideos provocou acelerado crescimento das papilas ruminais e das vilosidades do jejuno. O manano-frutoligossacarídeo não modificou os parâmetros avaliados. A suplementação de taurina não provocou alteração na fase de terminação de bovinos, entretanto, provocou queda no PH ruminal.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2019-03-07T13:57:10Z No. of bitstreams: 2 Tese - Natália Alves Costa - 2019.pdf: 8633931 bytes, checksum: e805e7100b0094eb60f4ee38d5c2bca4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2019-03-07T14:02:39Z (GMT) No. of bitstreams: 2 Tese - Natália Alves Costa - 2019.pdf: 8633931 bytes, checksum: e805e7100b0094eb60f4ee38d5c2bca4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-03-07T14:02:39Z (GMT). No. of bitstreams: 2 Tese - Natália Alves Costa - 2019.pdf: 8633931 bytes, checksum: e805e7100b0094eb60f4ee38d5c2bca4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-02-22Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Amparo à Pesquisa do Estado de Goiás - FAPEGapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Fisiológicas - Multicêntrico (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCrescimentoConfinamentoManejoGanho compensatórioSuplementaçãoGrowthFeedlotCompensatory gainManagementSupplementationCIENCIAS BIOLOGICAS::FISIOLOGIAManipulações alimentares em diferentes fases do desenvolvimento de bovinosFood manipulations in different stages of bovine developmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis1337318928839825463600600600600600-387277211782737340477377082474190182232075167498588264571-961409807440757778reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
dc.title.alternative.eng.fl_str_mv |
Food manipulations in different stages of bovine development |
title |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
spellingShingle |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos Costa, Natália Alves Crescimento Confinamento Manejo Ganho compensatório Suplementação Growth Feedlot Compensatory gain Management Supplementation CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
title_full |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
title_fullStr |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
title_full_unstemmed |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
title_sort |
Manipulações alimentares em diferentes fases do desenvolvimento de bovinos |
author |
Costa, Natália Alves |
author_facet |
Costa, Natália Alves |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nassar, Reginaldo Ferreira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2555785079833283 |
dc.contributor.referee1.fl_str_mv |
Pansani, Aline Patrícia |
dc.contributor.referee2.fl_str_mv |
Araujo Neto, Francisco Ribeiro de |
dc.contributor.referee3.fl_str_mv |
Castanheira, Marlos |
dc.contributor.referee4.fl_str_mv |
Santos, Fabiana Ramos dos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6892272740607732 |
dc.contributor.author.fl_str_mv |
Costa, Natália Alves |
contributor_str_mv |
Nassar, Reginaldo Ferreira Pansani, Aline Patrícia Araujo Neto, Francisco Ribeiro de Castanheira, Marlos Santos, Fabiana Ramos dos |
dc.subject.por.fl_str_mv |
Crescimento Confinamento Manejo Ganho compensatório Suplementação |
topic |
Crescimento Confinamento Manejo Ganho compensatório Suplementação Growth Feedlot Compensatory gain Management Supplementation CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.eng.fl_str_mv |
Growth Feedlot Compensatory gain Management Supplementation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
The objective of this study was to evaluate the physiological, biochemical and metabolic effects of milk restriction and prebiotic supplementation in suckling calves, and the same effects of supplementation of taurine in the bovine termination phase. Two experiments were executed to achieve the mentioned objectives. In the first trial it was used 20 crossbred calves with approximate initial weight of 38 kg and housed in individual pens for 56 days divided into two periods of 28 days each. The animals were allocaded in four experimental groups: animals receiving 6 L of milk in periods 1 and 2 (CON), animals with 50% milk restriction in the first period and re-alimented in the second period (RES), animals without restriction and supplemented of 5 g/day of mannanoligosaccharide (MOS) or mannan- frutoligosaccharide (MFOS). The weight gain and feed intake were verified. Serum was used to determinate glucose, lactate, creatinine, alkaline phosphatase, triglycerides, urea, toal protein and the hormones ghrelin and leptin. In the brain, in the paraventricular region of the hypothalamus, ghrelin receptor expression (GHS-R1a) was evaluated. The rumen and small intestine were used to evaluate the development of the gastrointestinal tract: rumen papilla length, villus height and intestinal crypt depth. We observed a lower weight gain of the restricted group in the first period and no difference in the weight gain among groups in the second period. It was observed that animals from the prebiotic supplemented (MOS) group showed a significant increase in jejunal villus height. Ruminal development was favored by the supplementation of mannanoligosaccharides (MOS group), which significantly increased ruminal papillae length. No difference was found for serum concentration of ghrelin and leptin among treatments in periods 1 and 2 (P> 0.05). Regarding the expression of ghrelin receptors in the paraventricular region of the hypothalamus, there was no difference between the groups evaluated. The second experiment was performed in 123 days with 80 finishing steers, mean initial weight of 507 kg and divided into groups supplemented with: 0; 0.025; 0.05; 0.075 and 0.1% taurine in the diet. Changes in performance were evaluated by weighing and control of the daily feed intake, carcass traits were evaluated after animals slaughter with data provided by the slaughter hose, and the metabolic changes were measured by plasma glucose, lactate, taurine and triglycerides values. Immediately after slaughter, a sample of Sternocephalicus ventrally muscle was collected to analyze the glycolytic potential. To complement the data from the second in vivo assay, an in vitro assay was performed using ruminal fluid cultures. Measurements included concentrations of VFA, pH, IVDMD and fermentative gas production for cultures containing 0; 0.025; 0.05; 0.075 and 0.1% taurine on the substrate. Supplementation of taurine did not affect the final characteristics of weight, HCW, DMI and carcass traits. Blood metabolites were not affected by administration of taurine. In addition, the production of in vitro gases, the concentrations of IVDMD and VFA’s were not affected by the addition of taurine. A linear response was detected for pH (P = 0.006) and with cattle consuming 0.1% taurine had the lowest pH. The compensatory gain presented by the restrictd milk animals, but did not accelerate the intestinal and ruminal development, while the supplementation of mananoligosaccharides caused an accelerated growth of the ruminal papillae and villi of the jejunum. Mannan-frutoligosaccharide supplementation did not accelerate the development of suckling calves. Taurine supplementation did not cause any change in the finishing phase of cattle, however, it caused a decrease in rumen pH. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-03-07T14:02:39Z |
dc.date.issued.fl_str_mv |
2019-02-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, N. A. Manipulações alimentares em diferentes fases do desenvolvimento de bovinos. 2019. 120 f. Tese (Doutorado em Ciências Fisiológicas) - Universidade Federal de Goiás, Goiânia, 2019. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/9318 |
identifier_str_mv |
COSTA, N. A. Manipulações alimentares em diferentes fases do desenvolvimento de bovinos. 2019. 120 f. Tese (Doutorado em Ciências Fisiológicas) - Universidade Federal de Goiás, Goiânia, 2019. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/9318 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
1337318928839825463 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
dc.relation.department.fl_str_mv |
-3872772117827373404 |
dc.relation.cnpq.fl_str_mv |
7737708247419018223 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 -961409807440757778 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências Fisiológicas - Multicêntrico (ICB) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ciências Biológicas - ICB (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
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Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
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tasesdissertacoes.bc@ufg.br |
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1798044426734403584 |