Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10603 |
Resumo: | MicroRNAs are small non-coding RNA molecules involved in the RNA interference phenomenon. These small RNAs contain between 18 and 25 nucleotides and are derived from precursors containing a hairpin structure. Their biogenesis and mode of action are well understood in plants and animals, but in fungi their biogenesis is still poorly understood. However, small RNAs similar to microRNAs (miRNAs-like) have been identified in various fungi, such as Neurospora crassa, Sclerotinia sclerotiorum, Penicillium marneffei, Trichoderma reesei and Paracoccidioides brasiliensis. Even though the amount of data on these molecules in the Fungi kingdom has been increasing, up to now there are no descriptions of microRNAs in important human pathogens such as Histoplasma capsulatum. Therefore, due to the absence of the characterization of this regulatory mechanism in this human pathogen, this study aimed to identify microRNAs in this pathogen and to infer the biological processes regulated by these interference RNAs in this fungus. In order to do so, the methodology employed in this study was the search for published microRNAs sequences for other fungi in the literature and in silico analysis to predict the homology of these known sequences to genome regions of two H. capsulaum strains H88 and G217B. After the bioinformatics analysis, we identified six microRNAs in H. capsulatum H88 and four microRNAs in H. capsulatum G217B .One of the microRNAs was conserved in both strains. This microRNA was selected for target investigation. We identified 551 targets in the H88 strain and 495 targets in the G217B strain. Biological processes identified as being regulated by this microRNA comprise the synthesis and the degradation of cell wall components, the energetic metabolism and DNA processing. The results imply the presence of microRNAs in this fungi and a high number of targets for each microRNA, which hints on the existence of a complex network of microRNA post-transcriptional regulation in H. capsulatum. |
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Soares, Célia Maria de Almeidahttp://lattes.cnpq.br/8539946335852637Curcio, Juliana Santana dehttp://lattes.cnpq.br/9577753232871923Tomazett, Mariana VieiraPereira, MaristelaSoares, Célia Maria de Almeidahttp://lattes.cnpq.br/2694084812144234Fernandes, Lucas Barros2020-09-08T14:33:51Z2020-09-08T14:33:51Z2020-03-06FERNANDES, L. B. Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum. 2020. 57 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10603MicroRNAs are small non-coding RNA molecules involved in the RNA interference phenomenon. These small RNAs contain between 18 and 25 nucleotides and are derived from precursors containing a hairpin structure. Their biogenesis and mode of action are well understood in plants and animals, but in fungi their biogenesis is still poorly understood. However, small RNAs similar to microRNAs (miRNAs-like) have been identified in various fungi, such as Neurospora crassa, Sclerotinia sclerotiorum, Penicillium marneffei, Trichoderma reesei and Paracoccidioides brasiliensis. Even though the amount of data on these molecules in the Fungi kingdom has been increasing, up to now there are no descriptions of microRNAs in important human pathogens such as Histoplasma capsulatum. Therefore, due to the absence of the characterization of this regulatory mechanism in this human pathogen, this study aimed to identify microRNAs in this pathogen and to infer the biological processes regulated by these interference RNAs in this fungus. In order to do so, the methodology employed in this study was the search for published microRNAs sequences for other fungi in the literature and in silico analysis to predict the homology of these known sequences to genome regions of two H. capsulaum strains H88 and G217B. After the bioinformatics analysis, we identified six microRNAs in H. capsulatum H88 and four microRNAs in H. capsulatum G217B .One of the microRNAs was conserved in both strains. This microRNA was selected for target investigation. We identified 551 targets in the H88 strain and 495 targets in the G217B strain. Biological processes identified as being regulated by this microRNA comprise the synthesis and the degradation of cell wall components, the energetic metabolism and DNA processing. The results imply the presence of microRNAs in this fungi and a high number of targets for each microRNA, which hints on the existence of a complex network of microRNA post-transcriptional regulation in H. capsulatum.MicroRNAs são pequenos transcritos não-codificantes envolvidos no fenômeno de interferência por RNA. Estes transcritos possuem entre 18 e 25 nucleotídeos e derivam de precursores com uma estrutura de grampo (hairpin). Sua biogênese e seu mecanismo de ação são bem compreendidos em plantas e animais, porém em fungos a biossíntese destes RNAs regulatórios ainda é pouco compreendida. Entretanto, pequenos RNAs já foram identificados em espécies de fungos, como Neurospora crassa, Sclerotinia sclerotiorum, Penicillium marneffei, Trichoderma reesei e Paracoccidioides brasiliensis. Embora os dados sobre estas moléculas no reino Fungi estejam aumentando, até o momento não existem descrições de microRNAs em importantes patógenos humanos como Histoplasma capsulatum. Portanto, devido à ausência da caracterização deste mecanismo regulatório neste patógeno humano, o presente trabalho visou identificar miRNAs neste fungo e inferir os possíveis processos biológicos regulados por esses RNAs de interferência. Para tal fim a metodologia empregada neste estudo foi a busca por sequências de microRNAs descritos em outros fungos e analises in silico para predizer a homologia destas sequências com regiões do genoma das linhagens de H. capsulatum H88 e G217B. Após as análises de bioinformática, identificamos seis microRNAs em H88 e quatro microRNAs em G217B. Destes, um microRNA era conservado em ambas as linhagens. Este microRNA foi selecionado para investigação de potenciais alvos. Foram identificados 551 alvos para este microRNA na linhagem H88 e 495 na linhagem G217B. Processos biológicos identificados como regulados por este microRNA incluem a síntese e a degradação de componentes de parede, o metabolismo energético e o processamento de DNA. Os resultados indicam a presença de microRNAs neste fungo e um grande número de alvos para cada microRNA, o que sugere uma complexa rede de regulação pós-transcricional efetuada por microRNAs em H. capsulatum.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-04T15:44:26Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Lucas Barros Fernandes - 2020.pdf: 2167346 bytes, checksum: 45355b89b0297c1cdffa37171c349c57 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-08T14:33:51Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Lucas Barros Fernandes - 2020.pdf: 2167346 bytes, checksum: 45355b89b0297c1cdffa37171c349c57 (MD5)Made available in DSpace on 2020-09-08T14:33:51Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Lucas Barros Fernandes - 2020.pdf: 2167346 bytes, checksum: 45355b89b0297c1cdffa37171c349c57 (MD5) Previous issue date: 2020-03-06Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia Molecular (ICB)UFGBrasilInstituto de Ciências Biológicas - ICB (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessMicroRNARegulação pós-transcricionalHistoplasma capsulatumFungo dimórficoPost-transcriptional regulationMicroRNAHistoplasma capsulatumDimorphic fungusCIENCIAS BIOLOGICASIdentificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatumIdentification and characterization of microRNAs in the dimorphic fungus Histoplasma capsulatuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis55500500500500241721reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/847bbeef-5476-461a-b587-25350dfbbda2/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/4955ff58-b45a-4d3c-a0ea-880e7dc05ef6/downloade39d27027a6cc9cb039ad269a5db8e34MD52ORIGINALDissertação - Lucas Barros Fernandes - 2020.pdfDissertação - Lucas Barros Fernandes - 2020.pdfapplication/pdf2167346http://repositorio.bc.ufg.br/tede/bitstreams/0a0ad6cb-1513-437e-8a31-e7ba7e41696e/download45355b89b0297c1cdffa37171c349c57MD53tede/106032020-09-08 11:33:51.646http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10603http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-08T14:33:51Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
dc.title.alternative.eng.fl_str_mv |
Identification and characterization of microRNAs in the dimorphic fungus Histoplasma capsulatum |
title |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
spellingShingle |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum Fernandes, Lucas Barros MicroRNA Regulação pós-transcricional Histoplasma capsulatum Fungo dimórfico Post-transcriptional regulation MicroRNA Histoplasma capsulatum Dimorphic fungus CIENCIAS BIOLOGICAS |
title_short |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
title_full |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
title_fullStr |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
title_full_unstemmed |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
title_sort |
Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum |
author |
Fernandes, Lucas Barros |
author_facet |
Fernandes, Lucas Barros |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8539946335852637 |
dc.contributor.advisor-co1.fl_str_mv |
Curcio, Juliana Santana de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9577753232871923 |
dc.contributor.referee1.fl_str_mv |
Tomazett, Mariana Vieira |
dc.contributor.referee2.fl_str_mv |
Pereira, Maristela |
dc.contributor.referee3.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2694084812144234 |
dc.contributor.author.fl_str_mv |
Fernandes, Lucas Barros |
contributor_str_mv |
Soares, Célia Maria de Almeida Curcio, Juliana Santana de Tomazett, Mariana Vieira Pereira, Maristela Soares, Célia Maria de Almeida |
dc.subject.por.fl_str_mv |
MicroRNA Regulação pós-transcricional Histoplasma capsulatum Fungo dimórfico |
topic |
MicroRNA Regulação pós-transcricional Histoplasma capsulatum Fungo dimórfico Post-transcriptional regulation MicroRNA Histoplasma capsulatum Dimorphic fungus CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Post-transcriptional regulation MicroRNA Histoplasma capsulatum Dimorphic fungus |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS |
description |
MicroRNAs are small non-coding RNA molecules involved in the RNA interference phenomenon. These small RNAs contain between 18 and 25 nucleotides and are derived from precursors containing a hairpin structure. Their biogenesis and mode of action are well understood in plants and animals, but in fungi their biogenesis is still poorly understood. However, small RNAs similar to microRNAs (miRNAs-like) have been identified in various fungi, such as Neurospora crassa, Sclerotinia sclerotiorum, Penicillium marneffei, Trichoderma reesei and Paracoccidioides brasiliensis. Even though the amount of data on these molecules in the Fungi kingdom has been increasing, up to now there are no descriptions of microRNAs in important human pathogens such as Histoplasma capsulatum. Therefore, due to the absence of the characterization of this regulatory mechanism in this human pathogen, this study aimed to identify microRNAs in this pathogen and to infer the biological processes regulated by these interference RNAs in this fungus. In order to do so, the methodology employed in this study was the search for published microRNAs sequences for other fungi in the literature and in silico analysis to predict the homology of these known sequences to genome regions of two H. capsulaum strains H88 and G217B. After the bioinformatics analysis, we identified six microRNAs in H. capsulatum H88 and four microRNAs in H. capsulatum G217B .One of the microRNAs was conserved in both strains. This microRNA was selected for target investigation. We identified 551 targets in the H88 strain and 495 targets in the G217B strain. Biological processes identified as being regulated by this microRNA comprise the synthesis and the degradation of cell wall components, the energetic metabolism and DNA processing. The results imply the presence of microRNAs in this fungi and a high number of targets for each microRNA, which hints on the existence of a complex network of microRNA post-transcriptional regulation in H. capsulatum. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-08T14:33:51Z |
dc.date.available.fl_str_mv |
2020-09-08T14:33:51Z |
dc.date.issued.fl_str_mv |
2020-03-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, L. B. Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum. 2020. 57 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10603 |
identifier_str_mv |
FERNANDES, L. B. Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum. 2020. 57 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10603 |
dc.language.iso.fl_str_mv |
por |
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por |
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55 |
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500 500 500 500 |
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24 |
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172 |
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1 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Genética e Biologia Molecular (ICB) |
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UFG |
dc.publisher.country.fl_str_mv |
Brasil |
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Instituto de Ciências Biológicas - ICB (RG) |
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Universidade Federal de Goiás |
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