Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás

Detalhes bibliográficos
Autor(a) principal: Faria, Adriana Marques
Data de Publicação: 2016
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/6710
Resumo: The closeness beteween wild and domestic animals with humans may result in common diseases involving these three groups, including zoonotic and antropozoonotic diseases. The study of bacteria and disease that affects animals is an important way to approach epidemiologic control and sanitary vigilance, as we do not understand the roll of wild animals in transmitting diseases among wild, exotic and domestic animals. The main objective of the present study was to search the presence of Escherichia coli and Salmonella sp. from rectal swabs of non-domesticated Suiformes in Commercial herds in Goiás State. It was collected rectal swabs of 100 Sus scrofa scrofa, 60 T. pecari and 30 P. tajacu, from different ages. The samples were used in order to obtain the isolation, antimicrobial profile, hemolisin test for E. coli and virulence and resistance gene presences in both bacteria. The serotyping was performed with polyvalent anti-O serum and the virulence and resistance gene deteccion by PCR technique. The results shown high rates of antimicrobial resistance and virulence and resistance genes in analyzed groups of E. coli. The isolation of Salmonella sp. occurred in one sample of S. scrofa, with high rates of antimicrobial resistance also, but no resistance and virulence genes were detected in this isolate. We must pay attention to the possibility of horizontal gene transference of virulence factors and resistance trough contaminated water and food, that may transform commensal bacteria in possible pathogenic agents. It is conclude: that Salmonella sp. is not commonly isolated from T. pecari and P. tajacu, showing low frequency of isolation in S. scrofa in these conditions; and E. coli with multidrug resistance patterns is isolated from S. scrofa, T. pecari and P. tajacu in commercial herds from Goiás State.
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spelling Linhares, Guido Fontgalland Coelhohttp://lattes.cnpq.br/6261928164195145Andrade, Maria Auxiliadorahttp://lattes.cnpq.br/9441751521255467Linhares, Guido Fontgalland CoelhoMoraes, Dunya Mara CardosoSantos, Adriana SilvaSilveira Neto, Osvaldo José daSilva, Virgínia Santiagohttp: //lattes.cnpq.br/3518219073254448Faria, Adriana Marques2017-01-11T09:54:12Z2016-12-06FARIA, A.M. Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás. 2016. 69 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6710The closeness beteween wild and domestic animals with humans may result in common diseases involving these three groups, including zoonotic and antropozoonotic diseases. The study of bacteria and disease that affects animals is an important way to approach epidemiologic control and sanitary vigilance, as we do not understand the roll of wild animals in transmitting diseases among wild, exotic and domestic animals. The main objective of the present study was to search the presence of Escherichia coli and Salmonella sp. from rectal swabs of non-domesticated Suiformes in Commercial herds in Goiás State. It was collected rectal swabs of 100 Sus scrofa scrofa, 60 T. pecari and 30 P. tajacu, from different ages. The samples were used in order to obtain the isolation, antimicrobial profile, hemolisin test for E. coli and virulence and resistance gene presences in both bacteria. The serotyping was performed with polyvalent anti-O serum and the virulence and resistance gene deteccion by PCR technique. The results shown high rates of antimicrobial resistance and virulence and resistance genes in analyzed groups of E. coli. The isolation of Salmonella sp. occurred in one sample of S. scrofa, with high rates of antimicrobial resistance also, but no resistance and virulence genes were detected in this isolate. We must pay attention to the possibility of horizontal gene transference of virulence factors and resistance trough contaminated water and food, that may transform commensal bacteria in possible pathogenic agents. It is conclude: that Salmonella sp. is not commonly isolated from T. pecari and P. tajacu, showing low frequency of isolation in S. scrofa in these conditions; and E. coli with multidrug resistance patterns is isolated from S. scrofa, T. pecari and P. tajacu in commercial herds from Goiás State.A proximidade de animais silvestres com animais domésticos e seres humanos pode resultar no aparecimento de doenças comuns a estes três grupos, incluindo doenças zoonóticas. O estudo de bactérias e doenças que acometem animais silvestres é importante para atuar na forma de controle epidemiológico e vigilância sanitária, visto que não se compreende o papel destes animais na transmissão de doenças entre outros animais silvestres, exóticos e domésticos. O presente estudo foi desenvolvido com o objetivo de pesquisara presença das bactérias Escherichia coli e Salmonella sp. em suabes retais provenientes de Suiformes não domesticados em criações comerciais no Estado de Goiás. Foram colhidos suabes retais de 100 Sus scrofa scrofa, 60 de Tayassu pecari e 30 de Pecari tajacu, de diferentes faixas etárias. À partir do material colhido fez-se o isolamento, perfil antimicrobiano, teste de hemólise para E. coli e detecção de genes de virulência e de resistência das bactérias. Para a detecção dos agentes, foi realizado exame bacteriológico convencional e ensaios de suscetibilidade a antimicrobianos. Foi realizadas a tipificação sorológica por teste sorológico com soro polivalente anti-O para a bactéria Salmonella e detecção de genes virulência e de resistência pela técnica de PCR nos isolados. Os resultados obtidos demonstraram altas taxas de resistência aos antimicrobianos e presença de genes de virulência em amostras de E. coli em todos os grupos testados. O isolamento de Salmonella sp. foi possível em uma amostra de S. scrofa, que apresentou também altas taxas de resistência à antimicrobianos, mas não houve detecção de genes de virulência ou de resistência neste isolado. Conclui-se que: nesta condição de criação Salmonella sp. não é isolada comumente de T. pecari e P. tajacu, sendo isolada em baixa frequência de S. scrofa; e que E. coli é isolada de S. scrofa, T. pecari e P. tajacu em rebanhos comerciais do Estado de Goiás, com múltiplos padrões de resistência antimicrobiana.Submitted by Erika Demachki (erikademachki@gmail.com) on 2017-01-10T16:57:36Z No. of bitstreams: 3 Tese - Adriana Marques Faria - 2016 - Parte 01.pdf: 5478875 bytes, checksum: ae9c08d62c1ad44305030954fe0b0a5e (MD5) Tese - Adriana Marques Faria - 2016 - Parte 02.pdf: 16834328 bytes, checksum: 2faf307ee33c6a223a00b491091a66f2 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-01-11T09:54:12Z (GMT) No. of bitstreams: 3 Tese - Adriana Marques Faria - 2016 - Parte 01.pdf: 5478875 bytes, checksum: ae9c08d62c1ad44305030954fe0b0a5e (MD5) Tese - Adriana Marques Faria - 2016 - Parte 02.pdf: 16834328 bytes, checksum: 2faf307ee33c6a223a00b491091a66f2 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-01-11T09:54:12Z (GMT). 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dc.title.por.fl_str_mv Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
dc.title.alternative.eng.fl_str_mv Escherichia coli and Salmonella sp. in native and exotic asymptomatic suiformes from comercial herds in Goiás state
title Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
spellingShingle Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
Faria, Adriana Marques
Enterobacteriáceas
PCR
Pecari tajacu
Sus scrofa scrofa
Tayassu pecari
Enterobacteriaceae
MEDICINA VETERINARIA::PATOLOGIA ANIMAL
title_short Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
title_full Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
title_fullStr Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
title_full_unstemmed Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
title_sort Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás
author Faria, Adriana Marques
author_facet Faria, Adriana Marques
author_role author
dc.contributor.advisor1.fl_str_mv Linhares, Guido Fontgalland Coelho
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6261928164195145
dc.contributor.advisor-co1.fl_str_mv Andrade, Maria Auxiliadora
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9441751521255467
dc.contributor.referee1.fl_str_mv Linhares, Guido Fontgalland Coelho
dc.contributor.referee2.fl_str_mv Moraes, Dunya Mara Cardoso
dc.contributor.referee3.fl_str_mv Santos, Adriana Silva
dc.contributor.referee4.fl_str_mv Silveira Neto, Osvaldo José da
dc.contributor.referee5.fl_str_mv Silva, Virgínia Santiago
dc.contributor.authorLattes.fl_str_mv http: //lattes.cnpq.br/3518219073254448
dc.contributor.author.fl_str_mv Faria, Adriana Marques
contributor_str_mv Linhares, Guido Fontgalland Coelho
Andrade, Maria Auxiliadora
Linhares, Guido Fontgalland Coelho
Moraes, Dunya Mara Cardoso
Santos, Adriana Silva
Silveira Neto, Osvaldo José da
Silva, Virgínia Santiago
dc.subject.por.fl_str_mv Enterobacteriáceas
PCR
Pecari tajacu
Sus scrofa scrofa
Tayassu pecari
topic Enterobacteriáceas
PCR
Pecari tajacu
Sus scrofa scrofa
Tayassu pecari
Enterobacteriaceae
MEDICINA VETERINARIA::PATOLOGIA ANIMAL
dc.subject.eng.fl_str_mv Enterobacteriaceae
dc.subject.cnpq.fl_str_mv MEDICINA VETERINARIA::PATOLOGIA ANIMAL
description The closeness beteween wild and domestic animals with humans may result in common diseases involving these three groups, including zoonotic and antropozoonotic diseases. The study of bacteria and disease that affects animals is an important way to approach epidemiologic control and sanitary vigilance, as we do not understand the roll of wild animals in transmitting diseases among wild, exotic and domestic animals. The main objective of the present study was to search the presence of Escherichia coli and Salmonella sp. from rectal swabs of non-domesticated Suiformes in Commercial herds in Goiás State. It was collected rectal swabs of 100 Sus scrofa scrofa, 60 T. pecari and 30 P. tajacu, from different ages. The samples were used in order to obtain the isolation, antimicrobial profile, hemolisin test for E. coli and virulence and resistance gene presences in both bacteria. The serotyping was performed with polyvalent anti-O serum and the virulence and resistance gene deteccion by PCR technique. The results shown high rates of antimicrobial resistance and virulence and resistance genes in analyzed groups of E. coli. The isolation of Salmonella sp. occurred in one sample of S. scrofa, with high rates of antimicrobial resistance also, but no resistance and virulence genes were detected in this isolate. We must pay attention to the possibility of horizontal gene transference of virulence factors and resistance trough contaminated water and food, that may transform commensal bacteria in possible pathogenic agents. It is conclude: that Salmonella sp. is not commonly isolated from T. pecari and P. tajacu, showing low frequency of isolation in S. scrofa in these conditions; and E. coli with multidrug resistance patterns is isolated from S. scrofa, T. pecari and P. tajacu in commercial herds from Goiás State.
publishDate 2016
dc.date.issued.fl_str_mv 2016-12-06
dc.date.accessioned.fl_str_mv 2017-01-11T09:54:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv FARIA, A.M. Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás. 2016. 69 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/6710
identifier_str_mv FARIA, A.M. Escherichia coli e Salmonella sp. em suiformes nativos e exóticos assintomáticos em criações comerciais do estado de Goiás. 2016. 69 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2016.
url http://repositorio.bc.ufg.br/tede/handle/tede/6710
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dc.relation.cnpq.fl_str_mv 5069770875075328004
dc.relation.sponsorship.fl_str_mv 2075167498588264571
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciência Animal (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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