In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products

Bibliographic Details
Main Author: Trombete, Felipe Machado
Publication Date: 2014
Other Authors: Santos, Thaís Barbosa, Direito, Glória Maria, Fraga, Marcelo Elias, Saldanha, Tatiana
Format: Article
Language: eng
Source: Pesquisa Agropecuária Tropical (Online)
Download full: https://revistas.ufg.br/pat/article/view/25449
Summary: Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.
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spelling In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-productsValidação intralaboratorial de metodologia para determinação de aflatoxinas B1, B2, G1 e G2 em trigo e produtos derivadosTriticum aestivum L.Aspergillusmycotoxinswheat branwhole wheat flour.Triticum aestivum L.Aspergillusmicotoxinasfarelo de trigofarinha de trigo integral.Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.Aflatoxinas são substâncias carcinogênicas de ocorrência natural, extremamente tóxicas para humanos, que têm sido identificadas no trigo e em produtos derivados. O uso de métodos analíticos confiáveis para avaliar e monitorar tais contaminantes é de grande importância. Este trabalho objetivou validar, intralaboratorialmente, uma metodologia para a determinação de aflatoxinas B1, B2, G1 e G2, em grãos de trigo, utilizando derivatização pré-coluna e quantificação por Cromatografia Líquida de Alta Eficiência com detecção por fluorescência (CLAE-DF). Três metodologias foram avaliadas e a que se adequou para tal propósito baseou-se na extração com clorofórmio, remoção dos interferentes por filtração, partição líquido-líquido com metanol-água-hexano e metanol-água-clorofórmio e derivatização pré-coluna com ácido trifluoracético. O método apresentou variação de repetibilidade inferior a 15% e recuperação na faixa de 70-110%, com limites de detecção e quantificação (0,6 µg kg-1 e 1,2 µg kg-1, respectivamente) inferiores ao nível máximo permitido pela Comissão Europeia (4,0 µg kg-1) e pela legislação brasileira (5,0 µg kg-1), para a presença de aflatoxinas em trigo e derivados. Por meio desta metodologia, foram quantificadas aflatoxinas em 20 amostras comerciais de grãos, farelo de trigo e farinha de trigo integral e refinada, destinadas ao consumo humano direto. Seis amostras (30%) foram positivas para aflatoxinas e todas apresentaram-se dentro do limite regulatório permitido pela legislação brasileira.Escola de Agronomia - Universidade Federal de Goiás2014-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMétodo quantitativoapplication/pdfhttps://revistas.ufg.br/pat/article/view/25449Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 44, n. 3, Jul./Sep. 2014; 255-262Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 44, n. 3, jul./set. 2014; 255-262Pesquisa Agropecuária Tropical; v. 44, n. 3, jul./set. 2014; 255-2621983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/25449/16857Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessTrombete, Felipe MachadoSantos, Thaís BarbosaDireito, Glória MariaFraga, Marcelo EliasSaldanha, Tatiana2020-07-10T16:33:41Zoai:ojs.revistas.ufg.br:article/25449Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2020-07-10T16:33:41Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
Validação intralaboratorial de metodologia para determinação de aflatoxinas B1, B2, G1 e G2 em trigo e produtos derivados
title In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
spellingShingle In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
Trombete, Felipe Machado
Triticum aestivum L.
Aspergillus
mycotoxins
wheat bran
whole wheat flour.
Triticum aestivum L.
Aspergillus
micotoxinas
farelo de trigo
farinha de trigo integral.
title_short In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
title_full In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
title_fullStr In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
title_full_unstemmed In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
title_sort In-house validation of a method for determining aflatoxins B1, B2, G1 and G2 in wheat and wheat by-products
author Trombete, Felipe Machado
author_facet Trombete, Felipe Machado
Santos, Thaís Barbosa
Direito, Glória Maria
Fraga, Marcelo Elias
Saldanha, Tatiana
author_role author
author2 Santos, Thaís Barbosa
Direito, Glória Maria
Fraga, Marcelo Elias
Saldanha, Tatiana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Trombete, Felipe Machado
Santos, Thaís Barbosa
Direito, Glória Maria
Fraga, Marcelo Elias
Saldanha, Tatiana
dc.subject.por.fl_str_mv Triticum aestivum L.
Aspergillus
mycotoxins
wheat bran
whole wheat flour.
Triticum aestivum L.
Aspergillus
micotoxinas
farelo de trigo
farinha de trigo integral.
topic Triticum aestivum L.
Aspergillus
mycotoxins
wheat bran
whole wheat flour.
Triticum aestivum L.
Aspergillus
micotoxinas
farelo de trigo
farinha de trigo integral.
description Aflatoxins are naturally occurring carcinogenic substances, extremely toxic to humans, which have been identified in wheat and wheat by-products. The use of reliable analytical methods to evaluate and monitor such contaminants is extremely important. This study aimed to in-house validate a methodology to determine aflatoxins B1, B2, G1 and G2 in wheat grains using pre-column derivatization and quantification by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD). Three methods were evaluated and the most suitable one was the method based on the extraction with chloroform, removal of interfering chemicals by filtration, liquid-liquid partition with hexane-methanol-water and methanol-water-chloroform and pre-column derivatization with trifluoroacetic acid. The method showed a Relative Standard Deviation lower than 15% and recovery values in the 70-110% range, with limits of detection and quantification (0.6 µg kg-1 and 1.2 µg kg-1, respectively) below the maximum level of aflatoxins allowed in wheat and wheat by-products by the European Commission (4.0 µg kg-1) and by the Brazilian legislation (5.0 µg kg-1). Using the validated method, aflatoxins were quantified in 20 commercial samples of wheat grains, wheat bran, whole wheat flour and refined wheat flour intended for direct human consumption. Six samples (30%) were positive for aflatoxins and all samples presented levels below the maximum limit stipulated by the Brazilian legislation.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Método quantitativo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/25449
url https://revistas.ufg.br/pat/article/view/25449
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/25449/16857
dc.rights.driver.fl_str_mv Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 44, n. 3, Jul./Sep. 2014; 255-262
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 44, n. 3, jul./set. 2014; 255-262
Pesquisa Agropecuária Tropical; v. 44, n. 3, jul./set. 2014; 255-262
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
_version_ 1789440241641193472

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