RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/17714 |
Resumo: | Protozoa of the rumen can represent 40% of total nitrogen and 60% of the final product of fermentation and it has cellulolytic activity. This study aimed to quantify and identify the ruminal protozoa populations from steers, raised exclusively on pastures during the dry season in the north of Minas Gerais, Brazil. Samples of ruminal fluid of 36 Nellore steers were collected directly from the rumen immediately after slaughter, during the early, middle or end of the dry season. For protozoa conservation, 1 mL of ruminal fluid was diluted in 9 mL of 10 % formaldehydes solution. Decimal dilutions were made in saline and the counting of small, medium and large protozoa was performed in Sedgewick Rafter chamber. The protozoa concentrations were transformed into log10 (x +1), subjected to variance analysis and the means were compared with Duncan test. The genus frequencies were compared with chi-square test. The concentration average of the total and of small and medium protozoa declined significantly over the course of the dry season; however, alterations in large protozoa concentrations were not detected over the three periods evaluated. A total of 7749 protozoa were identified, belonging to 17 different genera. Charonina spp. and Entodinium spp. had the highest occurrence during the three periods of dry season (P <0.05), suggesting the participation of these ciliates in rumen cattle fed exclusively on tropical pasture during this season. KEYWORDS: Cattle, nutrition, protozoa, ruminal juice, semiarid. |
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RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASONPROTOZOÁRIOS RUMINAIS DE NOVILHOS DE CORTE CRIADOS EM PASTAGEM TROPICAL DURANTE O PERÍODO SECOCattleanimal nutritionPecuária de corte. Ciliados. Microbiota ruminal. Semiárido. Brachiaria spp.Protozoa of the rumen can represent 40% of total nitrogen and 60% of the final product of fermentation and it has cellulolytic activity. This study aimed to quantify and identify the ruminal protozoa populations from steers, raised exclusively on pastures during the dry season in the north of Minas Gerais, Brazil. Samples of ruminal fluid of 36 Nellore steers were collected directly from the rumen immediately after slaughter, during the early, middle or end of the dry season. For protozoa conservation, 1 mL of ruminal fluid was diluted in 9 mL of 10 % formaldehydes solution. Decimal dilutions were made in saline and the counting of small, medium and large protozoa was performed in Sedgewick Rafter chamber. The protozoa concentrations were transformed into log10 (x +1), subjected to variance analysis and the means were compared with Duncan test. The genus frequencies were compared with chi-square test. The concentration average of the total and of small and medium protozoa declined significantly over the course of the dry season; however, alterations in large protozoa concentrations were not detected over the three periods evaluated. A total of 7749 protozoa were identified, belonging to 17 different genera. Charonina spp. and Entodinium spp. had the highest occurrence during the three periods of dry season (P <0.05), suggesting the participation of these ciliates in rumen cattle fed exclusively on tropical pasture during this season. KEYWORDS: Cattle, nutrition, protozoa, ruminal juice, semiarid.Protozoários do rúmen podem representar 40% do nitrogênio total e 60% do produto final da fermentação e apresentam atividade celulolítica. Neste estudo, objetivou-se quantificar e identificar as populações de protozoários no rúmen de novilhos, criados exclusivamente em pastagem, durante o período seco do ano, no Norte de Minas Gerais. Amostras do suco ruminal de 36 novilhos mestiços Nelore foram coletadas diretamente do rúmen, após o abate, durante o inicio, meio ou fim do período de estiagem. Para conservação dos protozoários, 1 mL do suco ruminal foi diluído em 9 mL de solução de formaldeido a 10%. Posteriormente, foram realizadas diluições decimais em solução salina e contagem de protozoários pequenos, médios e grandes em câmara de Sedgewick Rafter. As concentrações de protozoários foram transformadas em log10 (x+1) e submetidas à análise de variância e as médias comparadas com teste de Duncan. As frequências dos gêneros foram comparadas com o teste do qui-quadrado. As médias da concentração total e de pequenos e médios protozoários diminuíram significativamente com o decorrer do período seco; entretanto, os grandes protozoários não apresentaram alterações na concentração média durante os três períodos avaliados. Foram identificados 7749 protozoários, pertencentes a 17 gêneros diferentes. Observou-se que os gêneros Charonina e Entodinium apresentaram alta ocorrência no início, meio e final do período da seca (p<0,05), indicando a participação desses ciliados em bovinos alimentados exclusivamente a pasto, durante esse período do ano. PALAVRAS-CHAVE: Brachiaria spp., ciliados, microbiota ruminal, pecuária de corte, semiárido. Universidade Federal de Goiás2014-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa originalapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1771410.5216/cab.v15i3.17714Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 259-265Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 259-2651809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/17714/17066https://revistas.ufg.br/vet/article/view/17714/17067Silva, Kellerson LuizDuarte, Eduardo RobsonFreitas, Claudio Eduardo SilvaAbrão, Flávia OliveiraGeraseev, Luciana Castroinfo:eu-repo/semantics/openAccess2014-09-29T12:57:42Zoai:ojs.revistas.ufg.br:article/17714Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:52.347495Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON PROTOZOÁRIOS RUMINAIS DE NOVILHOS DE CORTE CRIADOS EM PASTAGEM TROPICAL DURANTE O PERÍODO SECO |
title |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
spellingShingle |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON Silva, Kellerson Luiz Cattle animal nutrition Pecuária de corte. Ciliados. Microbiota ruminal. Semiárido. Brachiaria spp. |
title_short |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
title_full |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
title_fullStr |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
title_full_unstemmed |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
title_sort |
RUMEN PROTOZOA OF STEERS RAISED ON TROPICAL PASTURE DURING DRY SEASON |
author |
Silva, Kellerson Luiz |
author_facet |
Silva, Kellerson Luiz Duarte, Eduardo Robson Freitas, Claudio Eduardo Silva Abrão, Flávia Oliveira Geraseev, Luciana Castro |
author_role |
author |
author2 |
Duarte, Eduardo Robson Freitas, Claudio Eduardo Silva Abrão, Flávia Oliveira Geraseev, Luciana Castro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Kellerson Luiz Duarte, Eduardo Robson Freitas, Claudio Eduardo Silva Abrão, Flávia Oliveira Geraseev, Luciana Castro |
dc.subject.por.fl_str_mv |
Cattle animal nutrition Pecuária de corte. Ciliados. Microbiota ruminal. Semiárido. Brachiaria spp. |
topic |
Cattle animal nutrition Pecuária de corte. Ciliados. Microbiota ruminal. Semiárido. Brachiaria spp. |
description |
Protozoa of the rumen can represent 40% of total nitrogen and 60% of the final product of fermentation and it has cellulolytic activity. This study aimed to quantify and identify the ruminal protozoa populations from steers, raised exclusively on pastures during the dry season in the north of Minas Gerais, Brazil. Samples of ruminal fluid of 36 Nellore steers were collected directly from the rumen immediately after slaughter, during the early, middle or end of the dry season. For protozoa conservation, 1 mL of ruminal fluid was diluted in 9 mL of 10 % formaldehydes solution. Decimal dilutions were made in saline and the counting of small, medium and large protozoa was performed in Sedgewick Rafter chamber. The protozoa concentrations were transformed into log10 (x +1), subjected to variance analysis and the means were compared with Duncan test. The genus frequencies were compared with chi-square test. The concentration average of the total and of small and medium protozoa declined significantly over the course of the dry season; however, alterations in large protozoa concentrations were not detected over the three periods evaluated. A total of 7749 protozoa were identified, belonging to 17 different genera. Charonina spp. and Entodinium spp. had the highest occurrence during the three periods of dry season (P <0.05), suggesting the participation of these ciliates in rumen cattle fed exclusively on tropical pasture during this season. KEYWORDS: Cattle, nutrition, protozoa, ruminal juice, semiarid. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa original |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/17714 10.5216/cab.v15i3.17714 |
url |
https://revistas.ufg.br/vet/article/view/17714 |
identifier_str_mv |
10.5216/cab.v15i3.17714 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/17714/17066 https://revistas.ufg.br/vet/article/view/17714/17067 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 259-265 Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 259-265 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874787632218112 |