ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR

Detalhes bibliográficos
Autor(a) principal: Linhares, Guido Fontgalland Coelho
Data de Publicação: 2006
Outros Autores: Santana, Angela Patrícia, Laueman, Lloyd H., Madruga, Cláudio R.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/288
Resumo: Six pairs of species-specific primers were designed from the alignment of the sequences of the SS rRNA gene obtained from the Genbank database for Babesia bigemina (accession number X59604) and for B. bovis (accession number U06105). Three pairs of primers were designed specifically for B. bigemina and another 3 sets of primers for B. bovis. All oligonucleotide sequences selected as primers were examined for similarities to other organisms through the Genbank Blast procedure and these 6 sets of primers demonstrated high level of specificity. The synthetic oligonucleotides were also tested for specificity by PCR assay using genomic DNA extracted from 40 isolates of B. bigemina and 30 from B. bovis, obtained in six different States of Brazil. All 6 sets of primers were validated as 100% specific for the respective parasite. The PCR amplified the expected fragments for each set of primers, as follows: a) B. bigemina: primers GAU5 forward/GAU6 reverse with amplicon of 1,124 bp; primers GAU5 forward/GAU8 reverse with amplicon of 458 bp; primers GAU7 forward/GAU6 reverse with amplicon of 685 bp; b) B. bovis: primers GAU9 forward/GAU10 reverse with amplicon of 541 bp; primers GAU9 forward/GAU 13 reverse with amplicon of 883 bp; primers SOGIN forward/GAU 10 with amplicon of 1211 bp. KEYWORDS: Babesia bovis, Babesia bigemina, SS rRNA gene, PCR, primers, bovine babesiosis.
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spelling ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCRSix pairs of species-specific primers were designed from the alignment of the sequences of the SS rRNA gene obtained from the Genbank database for Babesia bigemina (accession number X59604) and for B. bovis (accession number U06105). Three pairs of primers were designed specifically for B. bigemina and another 3 sets of primers for B. bovis. All oligonucleotide sequences selected as primers were examined for similarities to other organisms through the Genbank Blast procedure and these 6 sets of primers demonstrated high level of specificity. The synthetic oligonucleotides were also tested for specificity by PCR assay using genomic DNA extracted from 40 isolates of B. bigemina and 30 from B. bovis, obtained in six different States of Brazil. All 6 sets of primers were validated as 100% specific for the respective parasite. The PCR amplified the expected fragments for each set of primers, as follows: a) B. bigemina: primers GAU5 forward/GAU6 reverse with amplicon of 1,124 bp; primers GAU5 forward/GAU8 reverse with amplicon of 458 bp; primers GAU7 forward/GAU6 reverse with amplicon of 685 bp; b) B. bovis: primers GAU9 forward/GAU10 reverse with amplicon of 541 bp; primers GAU9 forward/GAU 13 reverse with amplicon of 883 bp; primers SOGIN forward/GAU 10 with amplicon of 1211 bp. KEYWORDS: Babesia bovis, Babesia bigemina, SS rRNA gene, PCR, primers, bovine babesiosis.Universidade Federal de Goiás2006-10-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/28810.5216/cab.v3i2.288Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 3 No. 2 (2002); 27-32Ciência Animal Brasileira / Brazilian Animal Science; v. 3 n. 2 (2002); 27-321809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/288/256Linhares, Guido Fontgalland CoelhoSantana, Angela PatríciaLaueman, Lloyd H.Madruga, Cláudio R.info:eu-repo/semantics/openAccess2013-10-12T20:38:51Zoai:ojs.revistas.ufg.br:article/288Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2013-10-12T20:38:51Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
title ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
spellingShingle ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
Linhares, Guido Fontgalland Coelho
title_short ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
title_full ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
title_fullStr ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
title_full_unstemmed ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
title_sort ASSESSMENT OF PRIMERS DESIGNED FROM THE SMALL RIBOSOMAL SUBUNIT RNA FOR SPECIFIC DISCRIMINATION BETWEEN BABESIA BIGEMINA AND BABESIA BOVIS BY PCR
author Linhares, Guido Fontgalland Coelho
author_facet Linhares, Guido Fontgalland Coelho
Santana, Angela Patrícia
Laueman, Lloyd H.
Madruga, Cláudio R.
author_role author
author2 Santana, Angela Patrícia
Laueman, Lloyd H.
Madruga, Cláudio R.
author2_role author
author
author
dc.contributor.author.fl_str_mv Linhares, Guido Fontgalland Coelho
Santana, Angela Patrícia
Laueman, Lloyd H.
Madruga, Cláudio R.
description Six pairs of species-specific primers were designed from the alignment of the sequences of the SS rRNA gene obtained from the Genbank database for Babesia bigemina (accession number X59604) and for B. bovis (accession number U06105). Three pairs of primers were designed specifically for B. bigemina and another 3 sets of primers for B. bovis. All oligonucleotide sequences selected as primers were examined for similarities to other organisms through the Genbank Blast procedure and these 6 sets of primers demonstrated high level of specificity. The synthetic oligonucleotides were also tested for specificity by PCR assay using genomic DNA extracted from 40 isolates of B. bigemina and 30 from B. bovis, obtained in six different States of Brazil. All 6 sets of primers were validated as 100% specific for the respective parasite. The PCR amplified the expected fragments for each set of primers, as follows: a) B. bigemina: primers GAU5 forward/GAU6 reverse with amplicon of 1,124 bp; primers GAU5 forward/GAU8 reverse with amplicon of 458 bp; primers GAU7 forward/GAU6 reverse with amplicon of 685 bp; b) B. bovis: primers GAU9 forward/GAU10 reverse with amplicon of 541 bp; primers GAU9 forward/GAU 13 reverse with amplicon of 883 bp; primers SOGIN forward/GAU 10 with amplicon of 1211 bp. KEYWORDS: Babesia bovis, Babesia bigemina, SS rRNA gene, PCR, primers, bovine babesiosis.
publishDate 2006
dc.date.none.fl_str_mv 2006-10-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/288
10.5216/cab.v3i2.288
url https://revistas.ufg.br/vet/article/view/288
identifier_str_mv 10.5216/cab.v3i2.288
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/288/256
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 3 No. 2 (2002); 27-32
Ciência Animal Brasileira / Brazilian Animal Science; v. 3 n. 2 (2002); 27-32
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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