OMPARISON BETWEEN TWO MEDIUM FOR EMBRYO TRANSFER IN MARES FROM MANGALARGA MARCHADOR BREED

• Autor(a) principal: Caiado, José Renato Costa
• Data de Publicação: 2009
• Outros Autores: van Tilburg, Mauricio Fraga, Fonseca, Francisco Aloísio, Silva, José Frederico Straggiotti, Fagundes, Bruno, Barreto, Marcus Antonio Pessanha
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/4514
• topic: Animal reproduction; reprodução animal

Different buffers are used in the solution for washing and embryo maintenance during the procedures of equine embryo transfer (EET). The buffers carbonate, phosphate, and zwitterionic are the most used. During the procedures of transference, it is common to keep the embryos into the maintenance solution during a time longer than previewed. This experiment was realized with the objective of comparing the costs and benefits of using two solutions with different buffers for embryo transfer in mares of the breeding Mangalarga Marchador. The solutions are DPBS (phosphate) and Embriocare® (zwitterionic). The embryos were divided randomly in two treatment , zwitterionic and phosphate, and kept in the solution for 0, 30, 60, and 120 minutes. The results indicate that both solutions are efficient (P > 0.05) in the processes of scanning, washing, maintenance at ambient temperature for different time periods (0 to 120 minutes), and inovulation of equine embryos under tropical conditions. The pregnancy rates using the solution were 65% and 69.7%. The greatest pregnancy rate using DPBS was obtained with embryos kept in the solution for 60 minutes (P < 0.05). Using zwitterionic buffer, the greatest rate of pregnancy was obtained with embryos kept during 30 minutes in the solution (P < 0.05). Since Embriocare® is more expensive, DPBS presented the best cost-benefit ratio.KEY WORDS: Embryo transfer, equine, Mangalarga Marchador, phosphate and zwitterionic buffer.