BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/3913 |
Resumo: | The objective of this study was to evaluate the effect of bronopol chemical in contrast to azidiol in the total bacterial counting (TBC) in raw milk samples submitted the different temperatures of storage. Four bands of samples storage temperature had been evaluated: T1 – 1.8 the 4.2 ºC; T2 – 6.4 the 8.3ºC; T3 – 12.1 the 14.5ºC; and T4 – 24 the 25.5ºC in three groups: Group 1 – control without chemical; Group 2 – liquid azidiol added of bromofenol blue; Group 3 – Bronopol®. The samples were analyzed during ten consecutive days, starting 24h after sampling. After analyzes by the flow citometry, have been considered raw milk samples of 14 expansion tanks that were divided in two levels of milk contamination: 106 CFU/mL. The bronopol effect was influenced by the raw milk contamination level. The Bronopol has bacteriostatic effect in milk samples with TBC below of 105 CFU/mL and bactericidal in samples with TBC above of 106 CFU/mL. The TBC gotten in raw milk samples conserved with bronopol was lower to that one gotten in the samples with azidiol. The bronopol effect on the TBC was influenced by the temperature and by the storage time of the sample. The Bronopol® bactericidal effect becomes impracticable its use in raw milk samples destined the TBC. The azidiol efficiency in the raw milk sample destined the CBT as bacteriostatic agent was dependent of the lesser storage temperature of the sample, independent of the initial contamination level. The raw milk sample destined the CBT can be analyzed in up to one week after harvested if the same one will be added of azidiol and kept under refrigeration in temperature between 1ºC and 4 ºC. KEY WORDS: Azidiol, bronopol, bacterial counting, raw milk, time, temperature. |
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BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILKCONSERVANTES BRONOPOL E AZIDIOL: INFLUÊNCIA DO BINÔMIO TEMPO/TEMPERATURA NA CONTAGEM BACTERIANA TOTAL DO LEITE CRUazidiolbronopoltempotemperaturacontagem bacterianaleite cruThe objective of this study was to evaluate the effect of bronopol chemical in contrast to azidiol in the total bacterial counting (TBC) in raw milk samples submitted the different temperatures of storage. Four bands of samples storage temperature had been evaluated: T1 – 1.8 the 4.2 ºC; T2 – 6.4 the 8.3ºC; T3 – 12.1 the 14.5ºC; and T4 – 24 the 25.5ºC in three groups: Group 1 – control without chemical; Group 2 – liquid azidiol added of bromofenol blue; Group 3 – Bronopol®. The samples were analyzed during ten consecutive days, starting 24h after sampling. After analyzes by the flow citometry, have been considered raw milk samples of 14 expansion tanks that were divided in two levels of milk contamination: 106 CFU/mL. The bronopol effect was influenced by the raw milk contamination level. The Bronopol has bacteriostatic effect in milk samples with TBC below of 105 CFU/mL and bactericidal in samples with TBC above of 106 CFU/mL. The TBC gotten in raw milk samples conserved with bronopol was lower to that one gotten in the samples with azidiol. The bronopol effect on the TBC was influenced by the temperature and by the storage time of the sample. The Bronopol® bactericidal effect becomes impracticable its use in raw milk samples destined the TBC. The azidiol efficiency in the raw milk sample destined the CBT as bacteriostatic agent was dependent of the lesser storage temperature of the sample, independent of the initial contamination level. The raw milk sample destined the CBT can be analyzed in up to one week after harvested if the same one will be added of azidiol and kept under refrigeration in temperature between 1ºC and 4 ºC. KEY WORDS: Azidiol, bronopol, bacterial counting, raw milk, time, temperature.O presente estudo objetivou avaliar o efeito do conservante bronopol em contraste ao azidiol na contagem bacteriana total (CBT) em amostras de leite cru submetidas a diferentes temperaturas de armazenamento. Avaliaram-se quatro faixas de temperatura de armazenamento de amostras: T1 – 1,8 a 4,2 ºC; T2 – 6,4 a 8,3ºC; T3 – 12,1 a 14,5ºC; e T4 – 24 a 25,5ºC, em três grupos: Grupo 1 – controle sem conservante; Grupo 2 – azidiol líquido + corante azul de bromofenol; Grupo 3 – bronopol®. As amostras foram analisadas durante dez dias consecutivos, iniciando 24 horas após colhidas. Após análise por citometria de fluxo pelo equipamento BactoScan FC, consideraram-se amostras de leite cru de quatorze tanques de expansão divididas em dois níveis de contaminação do leite: <105 e >106 UFC/mL. Houve efeito do conservante bronopol pelo nível de contaminação do leite cru. O bronopol possui efeito bacteriostático em amostras de leite com CBT abaixo de 105 UFC/mL e bactericida em amostras com CBT superiores a 106 UFC/mL. A CBT obtida nas amostras de leite cru conservadas com bronopol foi inferior àquela obtida nas amostras com azidiol. Ocorreu efeito do bronopol sobre a CBT pela temperatura e pelo tempo de estocagem da amostra. O efeito bactericida do bronopol® torna inviável sua utilização em amostras de leite cru destinadas à CBT. A eficiência do azidiol na amostra de leite cru destinada à CBT como agente bacteriostático foi dependente da menor temperatura de armazenamento da amostra, independente do nível de contaminação inicial. A amostra de leite cru destinada à CBT pode ser analisada em até uma semana depois de colhida, se for adicionada de azidiol e mantida sob refrigeração em temperatura entre 1ºC e 4 ºC.PALAVRAS-CHAVES: Azidiol, bronopol, contagem bacteriana, leite cru, tempo, temperatura.Universidade Federal de Goiás2009-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa Científicaapplication/pdfhttps://revistas.ufg.br/vet/article/view/391310.5216/cab.v10i2.3913Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 2 (2009); 627-633Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 2 (2009); 627-6331809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/3913/4857Martins, Márcio Eduardo PereiraNICOLAU, EDMAR SOARESMesquita, Albenones JoséNeves, Rodrigo Balduino SoaresOliveira, Jaison Pereirainfo:eu-repo/semantics/openAccess2013-11-07T12:06:46Zoai:ojs.revistas.ufg.br:article/3913Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:12.055825Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK CONSERVANTES BRONOPOL E AZIDIOL: INFLUÊNCIA DO BINÔMIO TEMPO/TEMPERATURA NA CONTAGEM BACTERIANA TOTAL DO LEITE CRU |
title |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
spellingShingle |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK Martins, Márcio Eduardo Pereira azidiol bronopol tempo temperatura contagem bacteriana leite cru |
title_short |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
title_full |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
title_fullStr |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
title_full_unstemmed |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
title_sort |
BRONOPOL AND AZIDIOL CHEMICALS: TIME AND TEMPERATURE INFLUENCE IN THE TOTAL BACTERIAL COUNT OF RAW MILK |
author |
Martins, Márcio Eduardo Pereira |
author_facet |
Martins, Márcio Eduardo Pereira NICOLAU, EDMAR SOARES Mesquita, Albenones José Neves, Rodrigo Balduino Soares Oliveira, Jaison Pereira |
author_role |
author |
author2 |
NICOLAU, EDMAR SOARES Mesquita, Albenones José Neves, Rodrigo Balduino Soares Oliveira, Jaison Pereira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Martins, Márcio Eduardo Pereira NICOLAU, EDMAR SOARES Mesquita, Albenones José Neves, Rodrigo Balduino Soares Oliveira, Jaison Pereira |
dc.subject.por.fl_str_mv |
azidiol bronopol tempo temperatura contagem bacteriana leite cru |
topic |
azidiol bronopol tempo temperatura contagem bacteriana leite cru |
description |
The objective of this study was to evaluate the effect of bronopol chemical in contrast to azidiol in the total bacterial counting (TBC) in raw milk samples submitted the different temperatures of storage. Four bands of samples storage temperature had been evaluated: T1 – 1.8 the 4.2 ºC; T2 – 6.4 the 8.3ºC; T3 – 12.1 the 14.5ºC; and T4 – 24 the 25.5ºC in three groups: Group 1 – control without chemical; Group 2 – liquid azidiol added of bromofenol blue; Group 3 – Bronopol®. The samples were analyzed during ten consecutive days, starting 24h after sampling. After analyzes by the flow citometry, have been considered raw milk samples of 14 expansion tanks that were divided in two levels of milk contamination: 106 CFU/mL. The bronopol effect was influenced by the raw milk contamination level. The Bronopol has bacteriostatic effect in milk samples with TBC below of 105 CFU/mL and bactericidal in samples with TBC above of 106 CFU/mL. The TBC gotten in raw milk samples conserved with bronopol was lower to that one gotten in the samples with azidiol. The bronopol effect on the TBC was influenced by the temperature and by the storage time of the sample. The Bronopol® bactericidal effect becomes impracticable its use in raw milk samples destined the TBC. The azidiol efficiency in the raw milk sample destined the CBT as bacteriostatic agent was dependent of the lesser storage temperature of the sample, independent of the initial contamination level. The raw milk sample destined the CBT can be analyzed in up to one week after harvested if the same one will be added of azidiol and kept under refrigeration in temperature between 1ºC and 4 ºC. KEY WORDS: Azidiol, bronopol, bacterial counting, raw milk, time, temperature. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/3913 10.5216/cab.v10i2.3913 |
url |
https://revistas.ufg.br/vet/article/view/3913 |
identifier_str_mv |
10.5216/cab.v10i2.3913 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/3913/4857 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 2 (2009); 627-633 Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 2 (2009); 627-633 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874784587153408 |