EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS

Detalhes bibliográficos
Autor(a) principal: Fonseca, Fabiane
Data de Publicação: 2007
Outros Autores: Hübner, Silvia de Oliveira, Vargas, Gilberto D’Avila, Fischer, Geferson, Vidor, Telmo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/1357
Resumo: The present study describes the evaluation of a filter paper blood sampling technique for assessment of the level of antibodies against the Newcastle disease virus (NDV) by hemagglutination inhibition (HI) test. Blood samples were collected from ninety eigth chickens with a known history of vaccination against NDV and analyzed by HI method. The blood was impregnated onto Whatman no. 1 filter paper with 3cm2 and also collected in tubes for obtaining serum. The papers were stored at room temperature and tested within fourty five days. The resulting sensitivity, specificity, positive predictive value and negative predictive value of the filter paper HI compared to serum HI was 89%, 73%, 76% and 87%, respectively. It was found index precision of the 80% and the correlation coefficient r = 0.81%. The overall average geometrical titer of antibodies was 33.1 and 33.6 in eluates and in sera, respectively. Association between the two methods was found to be highly significant (p < 0.001) when analyzed by Fishers test. The results indicate the potential use of filter paper blood sampling technique as an alternative to serum for detection of the antibodies against NDV. KEY-WORDS: Chicken, hemagglutination inhibition, serology.
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spelling EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUSAVALIAÇÃO DO USO DE SANGUE EM PAPEL-FILTRO PARA DETECÇÃO E QUANTIFICAÇÃO DE ANTICORPOS PARA O VÍRUS DA DOENÇA DE NEWCASTLEThe present study describes the evaluation of a filter paper blood sampling technique for assessment of the level of antibodies against the Newcastle disease virus (NDV) by hemagglutination inhibition (HI) test. Blood samples were collected from ninety eigth chickens with a known history of vaccination against NDV and analyzed by HI method. The blood was impregnated onto Whatman no. 1 filter paper with 3cm2 and also collected in tubes for obtaining serum. The papers were stored at room temperature and tested within fourty five days. The resulting sensitivity, specificity, positive predictive value and negative predictive value of the filter paper HI compared to serum HI was 89%, 73%, 76% and 87%, respectively. It was found index precision of the 80% and the correlation coefficient r = 0.81%. The overall average geometrical titer of antibodies was 33.1 and 33.6 in eluates and in sera, respectively. Association between the two methods was found to be highly significant (p < 0.001) when analyzed by Fishers test. The results indicate the potential use of filter paper blood sampling technique as an alternative to serum for detection of the antibodies against NDV. KEY-WORDS: Chicken, hemagglutination inhibition, serology.O presente trabalho descreve a utilização de sangue total em papel-filtro para avaliação dos níveis de anticorpos específicos contra o vírus da doença de Newcastle (NDV) através da técnica de inibição da hemaglutinação (HI). Coletaram-se e analisaram-se por HI amostras de sangue de noventa e oito galinhas, com um histórico conhecido de vacinação contra o NDV HI. O sangue foi impregnado em papel-filtro Wathman n° 1 com 3cm2 e também coletado em tubos para a obtenção de soro. Armazenaram-se os papéis à temperatura ambiente e procedeu-se a sua análise num período de até quarenta e cinco dias. A sensibilidade, a es¬pecificidade, o valor preditivo positivo e o valor preditivo negativo do HI mediante o uso de papel-filtro, comparado com o HI em que se utilizou soro, foram de 89%, 73%, 76% e 87 %, respectivamente. Detectaram-se um índice de pre¬cisão de 80% e um coeficiente de correlação r = 0,81%. O título médio geométrico de anticorpos foi 33,1% e 33,6 para eluídos de papel-filtro e os soros, respectivamente. A asso¬ciação entre os dois métodos foi altamente significativa (p< 0.001) quando analisada pelo teste de Fisher. Os resultados indicam o potencial da utilização do sangue em papel-filtro como uma alternativa ao soro para detecção de anticorpos contra o NDV. PALAVRAS-CHAVES: Galinha, inibição da hemaglutinação, sorologia.Universidade Federal de Goiás2007-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/135710.5216/cab.v8i2.1357Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 8 No. 2 (2007); 319-324Ciência Animal Brasileira / Brazilian Animal Science; v. 8 n. 2 (2007); 319-3241809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1357/1413Fonseca, FabianeHübner, Silvia de OliveiraVargas, Gilberto D’AvilaFischer, GefersonVidor, Telmoinfo:eu-repo/semantics/openAccess2007-07-25T17:50:13Zoai:ojs.revistas.ufg.br:article/1357Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2007-07-25T17:50:13Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
AVALIAÇÃO DO USO DE SANGUE EM PAPEL-FILTRO PARA DETECÇÃO E QUANTIFICAÇÃO DE ANTICORPOS PARA O VÍRUS DA DOENÇA DE NEWCASTLE
title EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
spellingShingle EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
Fonseca, Fabiane
title_short EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
title_full EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
title_fullStr EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
title_full_unstemmed EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
title_sort EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS
author Fonseca, Fabiane
author_facet Fonseca, Fabiane
Hübner, Silvia de Oliveira
Vargas, Gilberto D’Avila
Fischer, Geferson
Vidor, Telmo
author_role author
author2 Hübner, Silvia de Oliveira
Vargas, Gilberto D’Avila
Fischer, Geferson
Vidor, Telmo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Fabiane
Hübner, Silvia de Oliveira
Vargas, Gilberto D’Avila
Fischer, Geferson
Vidor, Telmo
description The present study describes the evaluation of a filter paper blood sampling technique for assessment of the level of antibodies against the Newcastle disease virus (NDV) by hemagglutination inhibition (HI) test. Blood samples were collected from ninety eigth chickens with a known history of vaccination against NDV and analyzed by HI method. The blood was impregnated onto Whatman no. 1 filter paper with 3cm2 and also collected in tubes for obtaining serum. The papers were stored at room temperature and tested within fourty five days. The resulting sensitivity, specificity, positive predictive value and negative predictive value of the filter paper HI compared to serum HI was 89%, 73%, 76% and 87%, respectively. It was found index precision of the 80% and the correlation coefficient r = 0.81%. The overall average geometrical titer of antibodies was 33.1 and 33.6 in eluates and in sera, respectively. Association between the two methods was found to be highly significant (p < 0.001) when analyzed by Fishers test. The results indicate the potential use of filter paper blood sampling technique as an alternative to serum for detection of the antibodies against NDV. KEY-WORDS: Chicken, hemagglutination inhibition, serology.
publishDate 2007
dc.date.none.fl_str_mv 2007-07-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/1357
10.5216/cab.v8i2.1357
url https://revistas.ufg.br/vet/article/view/1357
identifier_str_mv 10.5216/cab.v8i2.1357
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/1357/1413
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 8 No. 2 (2007); 319-324
Ciência Animal Brasileira / Brazilian Animal Science; v. 8 n. 2 (2007); 319-324
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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