Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus

Detalhes bibliográficos
Autor(a) principal: Fonseca Júnior, Antônio Augusto
Data de Publicação: 2024
Outros Autores: Laguardia-Nascimento, Mateus, Petrina Scotá Ferreira, Amanda, Rivetti Júnior, Anselmo Vasconcelos, Camargos, Marcelo Fernandes, da Silva, René Ribeiro
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/76304
Resumo: The prompt and accurate diagnosis of foot-and-mouth disease outbreaks is crucial to curb the swift transmission of its causative agent, the foot-and-mouth disease virus (FMDV), which poses a substantial threat to the nation’s livestock. This study aimed to perform a comparative analysis of various RT-qPCR methods for the detection of FMDV. The methods evaluated for the 3D region demonstrated similar sensitivity and specificity. Nevertheless, noticeable distinctions emerged in samples with low RNA concentration. While these methods can be interchangeably utilized with consistent outcomes when handling a large number of samples sent to the laboratory to pinpoint an outbreak, minor variations may assume significance when working with a limited quantity of samples containing low FMDV RNA copies.
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spelling Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virusComparação de sete diferentes RT-qPCR para detecção do virus da febre aftosaThe prompt and accurate diagnosis of foot-and-mouth disease outbreaks is crucial to curb the swift transmission of its causative agent, the foot-and-mouth disease virus (FMDV), which poses a substantial threat to the nation’s livestock. This study aimed to perform a comparative analysis of various RT-qPCR methods for the detection of FMDV. The methods evaluated for the 3D region demonstrated similar sensitivity and specificity. Nevertheless, noticeable distinctions emerged in samples with low RNA concentration. While these methods can be interchangeably utilized with consistent outcomes when handling a large number of samples sent to the laboratory to pinpoint an outbreak, minor variations may assume significance when working with a limited quantity of samples containing low FMDV RNA copies.O diagnóstico rápido e preciso de surtos de febre aftosa é crucial para conter a rápida transmissão de seu agente causador, o vírus da febre aftosa (FMDV), que representa uma ameaça significativa ao gado do país. Este estudo teve como objetivo realizar uma análise comparativa de vários métodos de RT-qPCR para a detecção do FMDV. Os métodos avaliados para a região 3D demonstraram sensibilidade e especificidade semelhantes. No entanto, foram observadas diferenças notáveis em amostras com baixa concentração de RNA. Embora esses métodos possam ser utilizados de forma intercambiável com resultados consistentes ao lidar com um grande número de amostras enviadas ao laboratório para identificar um surto, variações sutis podem assumir importância ao trabalhar com uma quantidade limitada de amostras contendo baixas cópias de RNA  Universidade Federal de Goiás2024-01-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/76304Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 25 (2024): Publicação continuaCiência Animal Brasileira / Brazilian Animal Science; v. 25 (2024): Publicação continua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/76304/40733https://revistas.ufg.br/vet/article/view/76304/40734https://revistas.ufg.br/vet/article/view/76304/40868https://revistas.ufg.br/vet/article/view/76304/40869Copyright (c) 2024 Ciência Animal Brasileira / Brazilian Animal Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFonseca Júnior, Antônio AugustoLaguardia-Nascimento, MateusPetrina Scotá Ferreira, AmandaRivetti Júnior, Anselmo VasconcelosCamargos, Marcelo Fernandesda Silva, René Ribeiro2024-03-27T21:45:20Zoai:ojs.revistas.ufg.br:article/76304Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:37.656117Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
Comparação de sete diferentes RT-qPCR para detecção do virus da febre aftosa
title Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
spellingShingle Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
Fonseca Júnior, Antônio Augusto
title_short Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
title_full Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
title_fullStr Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
title_full_unstemmed Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
title_sort Comparison of seven different RT-qPCR for diagnostic of Foot-and-Mouth disease virus
author Fonseca Júnior, Antônio Augusto
author_facet Fonseca Júnior, Antônio Augusto
Laguardia-Nascimento, Mateus
Petrina Scotá Ferreira, Amanda
Rivetti Júnior, Anselmo Vasconcelos
Camargos, Marcelo Fernandes
da Silva, René Ribeiro
author_role author
author2 Laguardia-Nascimento, Mateus
Petrina Scotá Ferreira, Amanda
Rivetti Júnior, Anselmo Vasconcelos
Camargos, Marcelo Fernandes
da Silva, René Ribeiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca Júnior, Antônio Augusto
Laguardia-Nascimento, Mateus
Petrina Scotá Ferreira, Amanda
Rivetti Júnior, Anselmo Vasconcelos
Camargos, Marcelo Fernandes
da Silva, René Ribeiro
description The prompt and accurate diagnosis of foot-and-mouth disease outbreaks is crucial to curb the swift transmission of its causative agent, the foot-and-mouth disease virus (FMDV), which poses a substantial threat to the nation’s livestock. This study aimed to perform a comparative analysis of various RT-qPCR methods for the detection of FMDV. The methods evaluated for the 3D region demonstrated similar sensitivity and specificity. Nevertheless, noticeable distinctions emerged in samples with low RNA concentration. While these methods can be interchangeably utilized with consistent outcomes when handling a large number of samples sent to the laboratory to pinpoint an outbreak, minor variations may assume significance when working with a limited quantity of samples containing low FMDV RNA copies.
publishDate 2024
dc.date.none.fl_str_mv 2024-01-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/76304
url https://revistas.ufg.br/vet/article/view/76304
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/76304/40733
https://revistas.ufg.br/vet/article/view/76304/40734
https://revistas.ufg.br/vet/article/view/76304/40868
https://revistas.ufg.br/vet/article/view/76304/40869
dc.rights.driver.fl_str_mv Copyright (c) 2024 Ciência Animal Brasileira / Brazilian Animal Science
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2024 Ciência Animal Brasileira / Brazilian Animal Science
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 25 (2024): Publicação continua
Ciência Animal Brasileira / Brazilian Animal Science; v. 25 (2024): Publicação continua
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874791302234112

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