VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/e-37893 |
Resumo: | The occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder . The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections.Keywords: cow; cell cytotoxicity; mammary gland; milk. |
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VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGSVIABILIDADE DE LEUCÓCITOS MAMÁRIOS BOVINOS DESAFIADOS COM DROGAS ANTI-MASTITEcowcytotoxicitymilk cellmammary gland.vacacitotoxicidade celularleiteglândula mamáriaThe occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder . The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections.Keywords: cow; cell cytotoxicity; mammary gland; milk.A ocorrência de células viáveis em secreções das glândulas mamárias atestam a funcionalidade do sistema de defesa. Este fato, juntamente com o medicamento aplicado, é fundamental para a cura do úbere. O objetivo deste trabalho foi avaliar a viabilidade in vitro de leucócitos do leite desafiados com quatro drogas anti-mastite intramamárias. Foram coletadas oito amostras de leite de vaca negativas no exame bacteriológico. As suspensões de células foram submetidas à avaliação da viabilidade pelo método de exclusão do Azul de Trypan pré-incubação e pós-incubação com os medicamentos. A taxa média de viabilidade pré-incubação (± desvio padrão) das células do leite foi 50,17% (± 6,50). As taxas médias de viabilidade pós-incubação (± desvio padrão) de grupo controle (desprovido de qualquer droga), Drogas 1 (cefalônio anidro), 2 (gentamicina), 3 (benzilpenicilina procaína associada a diidroestreptomicina naficilina) e 4 (cloxacilina benzatina) foram, respectivamente, 31,36% (± 3,61); 26.23% (± 4,49); 7,82% (± 2,33); 3,10% (± 0,94); e 30,33% (± 4,14). Os maiores prejuízos foram encontrados quando as células do leite foram desafiadas com as drogas 2 e 3. Embora a atividade antimicrobiana das drogas testadas não era avaliada, e um único aspecto da função das células tenha sido testado, concluiu-se que os tratamentos 2 e 3 poderiam prejudicar a funcionalidade de leucócitos do leite e fornecer uma resposta imunológica ineficaz, e uma resposta eficaz é indispensável para controlar as infecções mamárias.Palavras-chave: citotoxicidade celular; glândula mamária; leite; vacaUniversidade Federal de Goiás2017-02-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa CientíficaResarchapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-3789310.1590/cab18037893Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 18 (2017): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 18 (2017): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/vet/article/view/e-37893/22376https://revistas.ufg.br/vet/article/view/e-37893/22377Batista, Camila FreitasGomes, Renata CaminhaBlagitz, Maiara GarciaDella Libera, Alice Maria Melville Paivainfo:eu-repo/semantics/openAccess2023-06-07T18:48:26Zoai:ojs.revistas.ufg.br:article/37893Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2023-06-07T18:48:26Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false |
dc.title.none.fl_str_mv |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS VIABILIDADE DE LEUCÓCITOS MAMÁRIOS BOVINOS DESAFIADOS COM DROGAS ANTI-MASTITE |
title |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
spellingShingle |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS Batista, Camila Freitas cow cytotoxicity milk cell mammary gland. vaca citotoxicidade celular leite glândula mamária |
title_short |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
title_full |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
title_fullStr |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
title_full_unstemmed |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
title_sort |
VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS |
author |
Batista, Camila Freitas |
author_facet |
Batista, Camila Freitas Gomes, Renata Caminha Blagitz, Maiara Garcia Della Libera, Alice Maria Melville Paiva |
author_role |
author |
author2 |
Gomes, Renata Caminha Blagitz, Maiara Garcia Della Libera, Alice Maria Melville Paiva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Batista, Camila Freitas Gomes, Renata Caminha Blagitz, Maiara Garcia Della Libera, Alice Maria Melville Paiva |
dc.subject.por.fl_str_mv |
cow cytotoxicity milk cell mammary gland. vaca citotoxicidade celular leite glândula mamária |
topic |
cow cytotoxicity milk cell mammary gland. vaca citotoxicidade celular leite glândula mamária |
description |
The occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder . The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections.Keywords: cow; cell cytotoxicity; mammary gland; milk. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Científica Resarch |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-37893 10.1590/cab18037893 |
url |
https://revistas.ufg.br/vet/article/view/e-37893 |
identifier_str_mv |
10.1590/cab18037893 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-37893/22376 https://revistas.ufg.br/vet/article/view/e-37893/22377 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 18 (2017): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 18 (2017): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1789440222220517376 |