INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES

Detalhes bibliográficos
Autor(a) principal: Cunha, Melissa Savoia Castilho
Data de Publicação: 2010
Outros Autores: Marques, Mariana Groke, Lima, Alecsandra Sobreira, Nicacio, Alessandra Corallo, Assumpção, Mayra Elena Orrtiz Davila, Visintin, José Antonio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/3402
Resumo: Effects of in vitro fertilization (IVF) temperature and cumulus oophorus cells removal after IVF or 12 h of embryo culture (IVC) on polyspermy and embryo development rates were evaluated in swine. Oocytes and spermatozoa were incubated at 37 or 38.5ºC during IVF procedure. Cumulus oophorus cells were removed from 50% of zygotes of each group 8 hours after IVF and all zygotes were cultured with NCSU23 media. Polyspermy rates were assessed after 12 hours of IVC, when cumulus oophorus cells were removed from the rest of zygotes. In a second experiment, embryos remained in culture for the evaluation of embryo development. No effects of IVF temperature or cumulus oophorus cells removal were observed after 12 hours of IVC on polyspermy and embryo development (p
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spelling INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATESINFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIOAnimal ProductionProdução animalEffects of in vitro fertilization (IVF) temperature and cumulus oophorus cells removal after IVF or 12 h of embryo culture (IVC) on polyspermy and embryo development rates were evaluated in swine. Oocytes and spermatozoa were incubated at 37 or 38.5ºC during IVF procedure. Cumulus oophorus cells were removed from 50% of zygotes of each group 8 hours after IVF and all zygotes were cultured with NCSU23 media. Polyspermy rates were assessed after 12 hours of IVC, when cumulus oophorus cells were removed from the rest of zygotes. In a second experiment, embryos remained in culture for the evaluation of embryo development. No effects of IVF temperature or cumulus oophorus cells removal were observed after 12 hours of IVC on polyspermy and embryo development (pO objetivo deste trabalho foi avaliar o efeito da temperatura (37 ou 38,5°C) de fecundação in vitro (FIV) e da retirada das células do cumulus oophorus após a FIV ou após doze horas de cultivo embrionário (CIV), nos índices de poliespermia e no desenvolvimento de embriões suínos in vitro. Para a FIV, incubaram-se oócitos e espermatozoides em duas temperaturas (37 ou 38,5ºC). Após as oito horas de FIV, metade dos zigotos de cada grupo teve as células do cumulus oophorus retiradas e foi colocada em meio de cultivo NCSU23. Colocou-se a outra metade apenas em meio de cultivo e ambos os grupos foram mantidos nas mesmas temperaturas da FIV. Após doze horas de CIV, retiraram-se as células do restante dos zigotos e aferiram-se os índices de poliespermia, de todos os grupos. Na segunda etapa do experimento, os zigotos permaneceram em cultivo para avaliação do desenvolvimento embrionário. Não houve efeito da temperatura de FIV e da retirada das células do cumulus oophorus após doze horas de cultivo nos índices de poliespermia e no desenvolvimento embrionário (pUniversidade Federal de Goiás2010-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Cientificaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/340210.5216/cab.v11i2.3402Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 11 No. 2 (2010); 301-306Ciência Animal Brasileira / Brazilian Animal Science; v. 11 n. 2 (2010); 301-3061809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/3402/6833https://revistas.ufg.br/vet/article/view/3402/8155Cunha, Melissa Savoia CastilhoMarques, Mariana GrokeLima, Alecsandra SobreiraNicacio, Alessandra CoralloAssumpção, Mayra Elena Orrtiz DavilaVisintin, José Antonioinfo:eu-repo/semantics/openAccess2010-06-23T13:24:50Zoai:ojs.revistas.ufg.br:article/3402Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:07.279512Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
INFLUÊNCIA DA TEMPERATURA DE FECUNDAÇÃO IN VITRO E DAS CÉLULAS DO Cumulus oophorus SOBRE A TAXA DE POLISPERMIA E DESENVOLVIMENTO EMBRIONÁRIO
title INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
spellingShingle INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
Cunha, Melissa Savoia Castilho
Animal Production
Produção animal
title_short INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
title_full INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
title_fullStr INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
title_full_unstemmed INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
title_sort INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES
author Cunha, Melissa Savoia Castilho
author_facet Cunha, Melissa Savoia Castilho
Marques, Mariana Groke
Lima, Alecsandra Sobreira
Nicacio, Alessandra Corallo
Assumpção, Mayra Elena Orrtiz Davila
Visintin, José Antonio
author_role author
author2 Marques, Mariana Groke
Lima, Alecsandra Sobreira
Nicacio, Alessandra Corallo
Assumpção, Mayra Elena Orrtiz Davila
Visintin, José Antonio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Melissa Savoia Castilho
Marques, Mariana Groke
Lima, Alecsandra Sobreira
Nicacio, Alessandra Corallo
Assumpção, Mayra Elena Orrtiz Davila
Visintin, José Antonio
dc.subject.por.fl_str_mv Animal Production
Produção animal
topic Animal Production
Produção animal
description Effects of in vitro fertilization (IVF) temperature and cumulus oophorus cells removal after IVF or 12 h of embryo culture (IVC) on polyspermy and embryo development rates were evaluated in swine. Oocytes and spermatozoa were incubated at 37 or 38.5ºC during IVF procedure. Cumulus oophorus cells were removed from 50% of zygotes of each group 8 hours after IVF and all zygotes were cultured with NCSU23 media. Polyspermy rates were assessed after 12 hours of IVC, when cumulus oophorus cells were removed from the rest of zygotes. In a second experiment, embryos remained in culture for the evaluation of embryo development. No effects of IVF temperature or cumulus oophorus cells removal were observed after 12 hours of IVC on polyspermy and embryo development (p
publishDate 2010
dc.date.none.fl_str_mv 2010-06-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Cientifica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/3402
10.5216/cab.v11i2.3402
url https://revistas.ufg.br/vet/article/view/3402
identifier_str_mv 10.5216/cab.v11i2.3402
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/3402/6833
https://revistas.ufg.br/vet/article/view/3402/8155
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 11 No. 2 (2010); 301-306
Ciência Animal Brasileira / Brazilian Animal Science; v. 11 n. 2 (2010); 301-306
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874784421478400

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