CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER

Detalhes bibliográficos
Autor(a) principal: Cavalcante, José Maurício Maciel
Data de Publicação: 2014
Outros Autores: Brasil, Oscar Oliveira, Salgueiro, Cristiane Clemente de Mello, Salmito-Vanderley, Carminda Sandra Brito, Nunes, José Ferreira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/27834
Resumo: The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 oC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.KEYWORDS: ACP-102c; cryopreservation; ram semen.
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spelling CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDERCRIOPRESERVAÇÃO DO SÊMEN OVINO EM MEIO DILUENTE À BASE DE ÁGUA DE COCO EM PÓ (ACP-102c)Animal ReproductionReprodução AnimalThe aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 oC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.KEYWORDS: ACP-102c; cryopreservation; ram semen.O objetivo deste trabalho foi avaliar o diluente ACP-102c na criopreservação do sêmen ovino em comparação com o diluidor tris-glicose-gema (TRIS) e o sêmen fresco. Foram coletados 48 ejaculados de quatro ovinos, sendo tomadas duas alíquotas por ejaculado para diluição e criopreservação em ACP-102c ou TRIS e uma terceira alíquota utilizada para análise do sêmen fresco. O sêmen fresco e o criopreservado em ambos os diluidores foram avaliados para viabilidade, integridade de membrana plasmática e acrossomal, teste hiposmótico, fragmentação do DNA e de motilidade espermática. Após descongelamento, ambos os diluidores não diferiram para viabilidade espermática, integridade de membrana plasmática e acrossomal, fragmentação de DNA e nas variáveis quantitativas e qualitativas de velocidade espermática, mas diferiram no teste hiposmótico, motilidade total e progressiva e amplitude lateral da cabeça, bem como em todas as variáveis de motilidade avaliadas, exceto linearidade e progressividade, após duas horas de incubação à 37 oC. Houve variabilidade entre reprodutores na motilidade total e progressiva do sêmen criopreservado em ACP-102c após descongelamento. O diluidor ACP-102c conferiu menor proteção aos espermatozoides ovinos contra danos do congelamento quando comparado ao TRIS, mas o aprimoramento de sua formulação e protocolos mais adequados de congelação poderão torná-lo uma alternativa na congelação do sêmen ovino.PALAVRAS-CHAVE: ACP-102c; criopreservação; sêmen ovino. Universidade Federal de Goiás2014-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2783410.5216/cab.v15i3.27834Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 344-353Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 344-3531809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/27834/17088https://revistas.ufg.br/vet/article/view/27834/17089Cavalcante, José Maurício MacielBrasil, Oscar OliveiraSalgueiro, Cristiane Clemente de MelloSalmito-Vanderley, Carminda Sandra BritoNunes, José Ferreirainfo:eu-repo/semantics/openAccess2014-09-29T12:57:42Zoai:ojs.revistas.ufg.br:article/27834Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2014-09-29T12:57:42Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
CRIOPRESERVAÇÃO DO SÊMEN OVINO EM MEIO DILUENTE À BASE DE ÁGUA DE COCO EM PÓ (ACP-102c)
title CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
spellingShingle CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
Cavalcante, José Maurício Maciel
Animal Reproduction
Reprodução Animal
title_short CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
title_full CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
title_fullStr CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
title_full_unstemmed CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
title_sort CRYOPRESERVATION OF RAM SEMEN IN POWDERED COCONUT WATER (ACP-102c) BASED EXTENDER
author Cavalcante, José Maurício Maciel
author_facet Cavalcante, José Maurício Maciel
Brasil, Oscar Oliveira
Salgueiro, Cristiane Clemente de Mello
Salmito-Vanderley, Carminda Sandra Brito
Nunes, José Ferreira
author_role author
author2 Brasil, Oscar Oliveira
Salgueiro, Cristiane Clemente de Mello
Salmito-Vanderley, Carminda Sandra Brito
Nunes, José Ferreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cavalcante, José Maurício Maciel
Brasil, Oscar Oliveira
Salgueiro, Cristiane Clemente de Mello
Salmito-Vanderley, Carminda Sandra Brito
Nunes, José Ferreira
dc.subject.por.fl_str_mv Animal Reproduction
Reprodução Animal
topic Animal Reproduction
Reprodução Animal
description The aim of this study was to evaluate the ACP-102c extender in the cryopreservation of ram semen compared to tris-glucose-egg yolk (TRIS) extender and fresh semen. Forty-eight ejaculates were collected from four rams and two aliquots per ejaculate were taken for dilution and cryopreservation in ACP-102c or TRIS and a third aliquot used for the fresh semen analysis. Either the fresh semen and cryopreserved in both extenders were evaluated for viability, integrity of plasma and acrosomal membrane, hypoosmotic swelling test, DNA fragmentation and sperm motility. The extenders did not differ for sperm viability, acrosome and plasma membrane integrity, DNA fragmentation and quantitative and qualitative parameters of sperm velocity after thawing, but differed in hypoosmotic swelling test, total and progressive motility and lateral extent of the head as well as in all motility parameters evaluated (except linearity and straightness) after two hours of incubation at 37 oC. There was variability among rams in total and progressive motility of semen cryopreserved in ACP-102c after thawing. The ACP-102c extender showed less protection in the cryopreservation of ram sperm when compared to TRIS, but the improvement in its formulation and freezing protocols may make it an alternative to freezing ram semen.KEYWORDS: ACP-102c; cryopreservation; ram semen.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/27834
10.5216/cab.v15i3.27834
url https://revistas.ufg.br/vet/article/view/27834
identifier_str_mv 10.5216/cab.v15i3.27834
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/27834/17088
https://revistas.ufg.br/vet/article/view/27834/17089
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 344-353
Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 344-353
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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