BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL

Detalhes bibliográficos
Autor(a) principal: Nogueira, Adriana Hellmeister de Campos
Data de Publicação: 2009
Outros Autores: Pituco, Edviges Maristela, de Stefano, Eliana, Curci, Vera Cláudia Lorenzetti Magalhães, Cardoso, Tereza Cristina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/3782
Resumo: Bluetongue (BT) is an infectious, insect-born viral disease of ruminants. The causative agent of BT is bluetongue virus (BTV) that belongs to the family Reoviridae genus Orbivirus. Insect vectors in the genus Culicoides transmit this virus. BT affects domestic and wild ruminants, however small ruminants are considered the most affected specie. The aim of the study was to detect antibodies against BTV in commercial sheep farms, of the Northeastern region of Sao Paulo State, Brazil. A total of 1002 sera samples collected from adult sheep (above 1 year-old), comprising a total of 31 farms, were screened for the presence of BTV antibodies, by agar gel immunodiffusion test (AGID) and ELISA-CFS (Enzyme Linked Immunosorbent Assay – competitive solid phase), both produced by Pan American Center of FMDV. From a total of 1002 samples, 651 (65%) were positive by AGID and 742 (74.1%), were positive by ELISA-CFS. These results suggest that the BTV is widespread among farms, probably causing subclinical infections.KEY WORDS: AGID, bluetongue virus, ELISA-CFS, seroepidemiological survey.
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spelling BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZILDETECÇÃO DE ANTICORPOS CONTRA O VÍRUS DA LÍNGUA AZUL EM OVINOS NA REGIÃO DE ARAÇATUBA – SÃO PAULO, BRASILAnimal sanitySanidade AnimalBluetongue (BT) is an infectious, insect-born viral disease of ruminants. The causative agent of BT is bluetongue virus (BTV) that belongs to the family Reoviridae genus Orbivirus. Insect vectors in the genus Culicoides transmit this virus. BT affects domestic and wild ruminants, however small ruminants are considered the most affected specie. The aim of the study was to detect antibodies against BTV in commercial sheep farms, of the Northeastern region of Sao Paulo State, Brazil. A total of 1002 sera samples collected from adult sheep (above 1 year-old), comprising a total of 31 farms, were screened for the presence of BTV antibodies, by agar gel immunodiffusion test (AGID) and ELISA-CFS (Enzyme Linked Immunosorbent Assay – competitive solid phase), both produced by Pan American Center of FMDV. From a total of 1002 samples, 651 (65%) were positive by AGID and 742 (74.1%), were positive by ELISA-CFS. These results suggest that the BTV is widespread among farms, probably causing subclinical infections.KEY WORDS: AGID, bluetongue virus, ELISA-CFS, seroepidemiological survey.A língua azul é uma doença viral, cujo agente etiológico pertence à família Reoviridae, gênero Orbivirus, transmitida por um vetor (artrópode) hematófago, do gênero Culicoides. Os animais acometidos são ruminantes domésticos e selvagens, porém os pequenos ruminantes são os mais afetados. O estudo teve como objetivo detectar a presença de anticorpos para língua azul em ovinos da região de Araçatuba, por possuir um rebanho expressivo e condições climáticas favoráveis à multiplicação de insetos. Foram analisadas 1.002 amostras de soros ovinos, provenientes de 31 cabanhas, pelas provas de imunodifusão dupla em gel de ágar (AGID) e ELISA (Enzyme Linked immunosorbent Assay) de competição da fase sólida (ELISA CFS), provenientes do Centro Panamericano de Febre Aftosa. Desses soros, 651 (65%) foram reagentes ao vírus da língua azul, pela técnica de AGID, e 742 (74,1%) ao teste de ELISA–CFS. Esses resultados sugerem que o vírus da língua azul encontra-se disseminado nessas regiões, ocasionando infecções inaparentes. PALAVRAS-CHAVES: AGID, ELISA, inquérito soroepidemiológico, vírus da língua azul.Universidade Federal de Goiás2009-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/378210.5216/cab.v10i4.3782Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 4 (2009); 1271-1276Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 4 (2009); 1271-12761809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/3782/5949https://revistas.ufg.br/vet/article/view/3782/6041Nogueira, Adriana Hellmeister de CamposPituco, Edviges Maristelade Stefano, ElianaCurci, Vera Cláudia Lorenzetti MagalhãesCardoso, Tereza Cristinainfo:eu-repo/semantics/openAccess2009-12-20T12:07:51Zoai:ojs.revistas.ufg.br:article/3782Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2009-12-20T12:07:51Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
DETECÇÃO DE ANTICORPOS CONTRA O VÍRUS DA LÍNGUA AZUL EM OVINOS NA REGIÃO DE ARAÇATUBA – SÃO PAULO, BRASIL
title BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
spellingShingle BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
Nogueira, Adriana Hellmeister de Campos
Animal sanity
Sanidade Animal
title_short BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
title_full BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
title_fullStr BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
title_full_unstemmed BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
title_sort BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL
author Nogueira, Adriana Hellmeister de Campos
author_facet Nogueira, Adriana Hellmeister de Campos
Pituco, Edviges Maristela
de Stefano, Eliana
Curci, Vera Cláudia Lorenzetti Magalhães
Cardoso, Tereza Cristina
author_role author
author2 Pituco, Edviges Maristela
de Stefano, Eliana
Curci, Vera Cláudia Lorenzetti Magalhães
Cardoso, Tereza Cristina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Adriana Hellmeister de Campos
Pituco, Edviges Maristela
de Stefano, Eliana
Curci, Vera Cláudia Lorenzetti Magalhães
Cardoso, Tereza Cristina
dc.subject.por.fl_str_mv Animal sanity
Sanidade Animal
topic Animal sanity
Sanidade Animal
description Bluetongue (BT) is an infectious, insect-born viral disease of ruminants. The causative agent of BT is bluetongue virus (BTV) that belongs to the family Reoviridae genus Orbivirus. Insect vectors in the genus Culicoides transmit this virus. BT affects domestic and wild ruminants, however small ruminants are considered the most affected specie. The aim of the study was to detect antibodies against BTV in commercial sheep farms, of the Northeastern region of Sao Paulo State, Brazil. A total of 1002 sera samples collected from adult sheep (above 1 year-old), comprising a total of 31 farms, were screened for the presence of BTV antibodies, by agar gel immunodiffusion test (AGID) and ELISA-CFS (Enzyme Linked Immunosorbent Assay – competitive solid phase), both produced by Pan American Center of FMDV. From a total of 1002 samples, 651 (65%) were positive by AGID and 742 (74.1%), were positive by ELISA-CFS. These results suggest that the BTV is widespread among farms, probably causing subclinical infections.KEY WORDS: AGID, bluetongue virus, ELISA-CFS, seroepidemiological survey.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/3782
10.5216/cab.v10i4.3782
url https://revistas.ufg.br/vet/article/view/3782
identifier_str_mv 10.5216/cab.v10i4.3782
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/3782/5949
https://revistas.ufg.br/vet/article/view/3782/6041
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 4 (2009); 1271-1276
Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 4 (2009); 1271-1276
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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