EFFECT OF COLLING IN DIPLOID OOCYTES AFTER IN VITRO MATURATION

• Autor(a) principal: Luna, Hélder Silva e
• Data de Publicação: 2007
• Outros Autores: Ferrari, Iris, Rumpf, Rodolfo
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/2706
• topic: Bovinos; Reprodução

The present study aimed to verify the incidence of bovine diploid oocytes when cooled at various maturation stages. Bovine cumulus-oocyte complexes were recovered from ovaries at a local slaughterhouse and divided into five groups: control group (uncooled oocytes), 0/4 h group (oocytes cooled at 4ºC before the onset of maturation), 0/29 (composed of oocytes cooled at 29ºC before the onset of maturation), 12/4 (cooled 12 h at 4ºC after the onset of maturation), and 12/29 h group (cooled 12 h at 29ºC after the onset of maturation). the oocytes remained cooled for 45 min. in all groups, the oocytes completed 24 h maturation. Subsequently, the cumulus cells were removed, and the denuded oocytes fixed on slides and stained with aceto-orcein. No differences (P > 0.05) in the incidence of diploid metaphase ii oocytes were observed between the control group (6.0%) and oocytes cooled at 4ºC and 29ºC before (8.9% and 8.0%) and after 12 h the onset of maturation (3.9% and 0.0%). These results suggest that the nuclear stage at which bovine oocytes are cooled does not affect the incidence of diploid oocytes after 24 h maturation. Key-Words: Bovine, cooling, diploid, in vitro maturation, oocytes.