EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)

Detalhes bibliográficos
Autor(a) principal: Fernandes, Maureen Hoch Vieira
Data de Publicação: 2015
Outros Autores: Ferreira, Lilian das Neves, Vargas, Gilberto D'Avila, Fischer, Geferson, Hübner, Silvia de Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/23458
Resumo: The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-?. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-? mRNA in the immunized animals. Increased levels of IFN-? mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 ?g/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-? to CPV were not influenced by the presence of WEBP. Keywords: adjuvant; canine coronavirus; canine parvovirus; propolis; vaccine.
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spelling EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)EFEITO DO EXTRATO AQUOSO DE PRÓPOLIS MARROM SOBRE A PRODUÇÃO DE IFN-? APÓS IMUNIZAÇÃO CONTRA PARVOVÍRUS CANINO (CPV) E CORONAVÍRUS CANINO (CCoV)apiculturePrópolisVacinaAdjuvanteParvovírus caninoCoronavírus caninoThe purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-?. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-? mRNA in the immunized animals. Increased levels of IFN-? mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 ?g/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-? to CPV were not influenced by the presence of WEBP. Keywords: adjuvant; canine coronavirus; canine parvovirus; propolis; vaccine.O objetivo deste estudo foi avaliar a capacidade adjuvante imunoestimulatória do extrato aquoso de própolis marrom (EAPM) quando associado a uma vacina contra parvovírus canino (CPV) e coronavírus canino (CCoV), com relação à produção de IFN-?. Camundongos foram vacinados com CPV e CCoV (3,0x106 TCDI50) em associação ou não com 400 ?g/dose de EAPM. Trinta dias após a terceira dose foi realizado cultivo de esplenócitos para mensuração dos níveis de expressão de mRNA para IFN-? nos animais imunizados. O aumento nos níveis de expressão de mRNA para IFN-? para CCoV nos esplenócitos dos camundongos inoculados com a vacina contendo 400 ?g/dose de EAPM foi evidenciado por RT-PCR, demonstrando a capacidade da própolis em estimular a resposta imune celular contra os antígenos desse vírus. Ao contrário, os níveis de IFN-? para CPV não sofreram influência da presença do EAPM. Palavras-Chave: adjuvante; coronavírus canino; parvovírus canino; própolis; vacina. Universidade Federal de Goiás2015-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2345810.1590/cab.v16i2.23458Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 235-242Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 235-2421809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/23458/18495https://revistas.ufg.br/vet/article/view/23458/18496Fernandes, Maureen Hoch VieiraFerreira, Lilian das NevesVargas, Gilberto D'AvilaFischer, GefersonHübner, Silvia de Oliveirainfo:eu-repo/semantics/openAccess2015-05-22T12:39:37Zoai:ojs.revistas.ufg.br:article/23458Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2015-05-22T12:39:37Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
EFEITO DO EXTRATO AQUOSO DE PRÓPOLIS MARROM SOBRE A PRODUÇÃO DE IFN-? APÓS IMUNIZAÇÃO CONTRA PARVOVÍRUS CANINO (CPV) E CORONAVÍRUS CANINO (CCoV)
title EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
spellingShingle EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
Fernandes, Maureen Hoch Vieira
apiculture
Própolis
Vacina
Adjuvante
Parvovírus canino
Coronavírus canino
title_short EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
title_full EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
title_fullStr EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
title_full_unstemmed EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
title_sort EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)
author Fernandes, Maureen Hoch Vieira
author_facet Fernandes, Maureen Hoch Vieira
Ferreira, Lilian das Neves
Vargas, Gilberto D'Avila
Fischer, Geferson
Hübner, Silvia de Oliveira
author_role author
author2 Ferreira, Lilian das Neves
Vargas, Gilberto D'Avila
Fischer, Geferson
Hübner, Silvia de Oliveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Maureen Hoch Vieira
Ferreira, Lilian das Neves
Vargas, Gilberto D'Avila
Fischer, Geferson
Hübner, Silvia de Oliveira
dc.subject.por.fl_str_mv apiculture
Própolis
Vacina
Adjuvante
Parvovírus canino
Coronavírus canino
topic apiculture
Própolis
Vacina
Adjuvante
Parvovírus canino
Coronavírus canino
description The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-?. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-? mRNA in the immunized animals. Increased levels of IFN-? mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 ?g/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-? to CPV were not influenced by the presence of WEBP. Keywords: adjuvant; canine coronavirus; canine parvovirus; propolis; vaccine.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/23458
10.1590/cab.v16i2.23458
url https://revistas.ufg.br/vet/article/view/23458
identifier_str_mv 10.1590/cab.v16i2.23458
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/23458/18495
https://revistas.ufg.br/vet/article/view/23458/18496
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 235-242
Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 235-242
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1789440221426745344

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