EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR

Detalhes bibliográficos
Autor(a) principal: Costa, Deyse Naira Mascarenhas
Data de Publicação: 2014
Outros Autores: Silva, Daniel Anderson Martiliano, Boakari, Yatta Linhares, Ferreira, Siluana Benvindo, Castelo Branco, Marlon Araújo, de Souza, José Adalmir Torres
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/25327
Resumo: The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.KEYWORDS: cryopreservation, equine, extender, semen.
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spelling EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOREFICIÊNCIA DOS DILUIDORES TRIS E BOTU-CRIO® SOBRE OS PARÂMETROS SEMINAIS DE GARANHÕES DAS RAÇAS QUARTO DE MILHA E MANGALARGA MARCHADORAnimal SanityEquinoSêmenDiluenteCriopreservaçãoThe limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.KEYWORDS: cryopreservation, equine, extender, semen.O principal fator a limitar o processo de criopreservação de sêmen equino está relacionado à própria espécie, por apresentar uma grande variabilidade nas características dos ejaculados pós-descongelação. O objetivo desta pesquisa foi avaliar a viabilidade espermática pós-descongelação, do sêmen de garanhões das raças Quarto de Milha e Mangalarga Marchador utilizando-se dois diluentes (Tris e Botu-crio®) na criopreservação. Para tanto, foram analisadas as características físicas do sêmen fresco, funcionalidade da membrana espermática pelo Teste Hiposmótico (HOST), motilidade total e vigor pelo Teste de Termoresistência (TTR), motilidade progressiva pelo sistema computadorizado CASA (Computer Assisted Sêmen Anlyses) e integridade da membrana espermática, do acrossoma e funcionalidade de mitocôndria por microscopia de epifluorescência. O diluente Botu-crio® preservou melhor os valores de motilidade e vigor e a integridade de membrana plasmática, pós-descongelamento. Não houve diferença significativa entre as raças quanto à motilidade total e vigor no sêmen fresco, bem como para a análise desses parâmetros no Teste de Termoresistência pós-descongelamento. Garanhões da raça Quarto de Milha apresentaram percentual maior de defeitos maiores na análise de patologia espermática.PALAVRAS-CHAVE: criopreservação, diluente, equino, sêmen  Universidade Federal de Goiás2014-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Ciêntíficaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2532710.5216/cab.v15i3.25327Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 322-329Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 322-3291809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/25327/17082https://revistas.ufg.br/vet/article/view/25327/17083Costa, Deyse Naira MascarenhasSilva, Daniel Anderson MartilianoBoakari, Yatta LinharesFerreira, Siluana BenvindoCastelo Branco, Marlon Araújode Souza, José Adalmir Torresinfo:eu-repo/semantics/openAccess2014-09-29T12:57:42Zoai:ojs.revistas.ufg.br:article/25327Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2014-09-29T12:57:42Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
EFICIÊNCIA DOS DILUIDORES TRIS E BOTU-CRIO® SOBRE OS PARÂMETROS SEMINAIS DE GARANHÕES DAS RAÇAS QUARTO DE MILHA E MANGALARGA MARCHADOR
title EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
spellingShingle EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
Costa, Deyse Naira Mascarenhas
Animal Sanity
Equino
Sêmen
Diluente
Criopreservação
title_short EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
title_full EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
title_fullStr EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
title_full_unstemmed EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
title_sort EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR
author Costa, Deyse Naira Mascarenhas
author_facet Costa, Deyse Naira Mascarenhas
Silva, Daniel Anderson Martiliano
Boakari, Yatta Linhares
Ferreira, Siluana Benvindo
Castelo Branco, Marlon Araújo
de Souza, José Adalmir Torres
author_role author
author2 Silva, Daniel Anderson Martiliano
Boakari, Yatta Linhares
Ferreira, Siluana Benvindo
Castelo Branco, Marlon Araújo
de Souza, José Adalmir Torres
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Deyse Naira Mascarenhas
Silva, Daniel Anderson Martiliano
Boakari, Yatta Linhares
Ferreira, Siluana Benvindo
Castelo Branco, Marlon Araújo
de Souza, José Adalmir Torres
dc.subject.por.fl_str_mv Animal Sanity
Equino
Sêmen
Diluente
Criopreservação
topic Animal Sanity
Equino
Sêmen
Diluente
Criopreservação
description The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.KEYWORDS: cryopreservation, equine, extender, semen.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Ciêntífica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/25327
10.5216/cab.v15i3.25327
url https://revistas.ufg.br/vet/article/view/25327
identifier_str_mv 10.5216/cab.v15i3.25327
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/25327/17082
https://revistas.ufg.br/vet/article/view/25327/17083
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 15 No. 3 (2014); 322-329
Ciência Animal Brasileira / Brazilian Animal Science; v. 15 n. 3 (2014); 322-329
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1789440221508534272

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