Avaliação do dano genético em células sanguíneas de quirópteros de áreas urbanas e não urbanas

• Main Author: Assunção, Dornelles
• Publication Date: 2016
• Format: Master thesis
• Language: por
• Source: Biblioteca Digital de Teses e Dissertações da UFG
• Download full: http://repositorio.bc.ufg.br/tede/handle/tede/5959
• topic: Genotoxicidade; Quirópteros; Ensaio cometa; Ecologia urbana; Genotoxicity; Chiroptera; Comet assay; Urban ecology; CIENCIAS BIOLOGICAS::ZOOLOGIA

The evaluation of DNA damage was carried out with the comet assay from blood samples of three bat species sampled at municipal parks of Goiania and Cerrado remnants, located in rural areas of the municipalities of Bela Vista and Urutaí, Goias state. The species sampled in the urban area were Artibeus lituratus, Artibeus planirostris and Platyrrhinus lineatus. In non-urban areas, only Artibeus planirostris was sampled. These frugivorous species are abundant and well adapted to different environments, including urban and agricultural areas. The remaining vegetation of the urban environment was very impacted and it was included in the most urbanized area of the city, with heavy traffic of vehicles, large concentration of buildings and high population density. Sampling in non-urban environments were held in Cerrado fragments in a rural area surrounded by pasture and agricultural activities. Thus, the DNA damage found in the urban environment bats were compared to verify whether there was significant variation among the parks, between species and if weight influenced the variation of the DNA damage. The DNA damage obtained for A. planirostris allowed to compare the urban and non-urban environment to identify in which of the environments this species presented higher DNA damages. The results showed that in the nonurban environment, the DNA damage was higher than in the urban environment being statistically significant. Moreover, in the urban environment, the increased DNA damage was associated with a reduction in area of the parks. In general, in the urban environment, the DNA damage may be linked indirectly to the edge effect and other environmental stressors, while in non-urban environment, higher levels of DNA damage may be related to pesticides used in the agricultural activities.