Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/883 |
Resumo: | The canine gland has drawn interest for research due to its similarities with the human prostate and the great incidence of lesions. Moreover, the canine prostate shows high incidence of diseases. The main lesions that affect the prostate are prostatitis, benign prostatic hyperplasia (BPH), cysts and adenocarcinoma. Recently attention has been given to lesions considered premalignant such as prostatic intraepithelial neoplasia (PIN) and proliferative inflammatory atrophy (PIA), both studied in the human gland and also found in the canine prostate. In order to evaluate the development of prostate cancer starting as premalignant lesions, some immunohistochemical markers are employed, such as tissue inhibitors of metalloproteinases (TIMP), which have a key role in regulating the catalytic action of metalloproteinases (MMP), and transforming growth factor-β (TGF-β), that induces angiogenesis and inhibits cell proliferation and is considered a mediator of prostate growth. The objective of this study was to investigate the expression of TIMP-1, TIMP-2 and TGF-β in canine normal prostate tissue and with proliferative lesions. For this, 150 adult canine prostates were obtained from postmortem examinations. After microscopic evaluation 54 glands, compatible with normal, epithelial BPH, stromal BPH, PIA, PIN and adenocarcinoma were selected and used to make tissue microarray block (Tissue Microarray - TMA). TMA slides were subjected to immunohistochemistry with anti-TIMP-1, anti-TIMP-2 and anti-TGF-β, to assess staining intensity of epithelial cells and stromal cells.Cytoplasmatic staining of canine prostate cells by TIMP-1, TIMP-2 and TGF-β was observed, with TIMP-1 and TIMP-2 being more expressed in premalignant and malignant lesions, while TGF-β was expressed mainly by normal tissue and BPH. Furthermore, there were differences in the expression between epithelial and stromal cells. |
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MOURA, Veridiana Maria Brianezi Dignani dehttp://lattes.cnpq.br/8773201078957745MENEZES, Liliana Borges dehttp://lattes.cnpq.br/2012543423092393BRITO, Luiz Augusto Batistahttp://lattes.cnpq.br/3212353728321020http://lattes.cnpq.br/0730928874381169TOLEDO, Denise Caroline2014-07-29T15:07:41Z2012-09-142012-03-08TOLEDO, Denise Caroline. Expression of tansforming growth factor B and tissue inhibitor of metalloproteianse 1 and 2 in normal canine prostates and with proliferative lessions. 2012. 81 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2012.http://repositorio.bc.ufg.br/tede/handle/tde/883The canine gland has drawn interest for research due to its similarities with the human prostate and the great incidence of lesions. Moreover, the canine prostate shows high incidence of diseases. The main lesions that affect the prostate are prostatitis, benign prostatic hyperplasia (BPH), cysts and adenocarcinoma. Recently attention has been given to lesions considered premalignant such as prostatic intraepithelial neoplasia (PIN) and proliferative inflammatory atrophy (PIA), both studied in the human gland and also found in the canine prostate. In order to evaluate the development of prostate cancer starting as premalignant lesions, some immunohistochemical markers are employed, such as tissue inhibitors of metalloproteinases (TIMP), which have a key role in regulating the catalytic action of metalloproteinases (MMP), and transforming growth factor-β (TGF-β), that induces angiogenesis and inhibits cell proliferation and is considered a mediator of prostate growth. The objective of this study was to investigate the expression of TIMP-1, TIMP-2 and TGF-β in canine normal prostate tissue and with proliferative lesions. For this, 150 adult canine prostates were obtained from postmortem examinations. After microscopic evaluation 54 glands, compatible with normal, epithelial BPH, stromal BPH, PIA, PIN and adenocarcinoma were selected and used to make tissue microarray block (Tissue Microarray - TMA). TMA slides were subjected to immunohistochemistry with anti-TIMP-1, anti-TIMP-2 and anti-TGF-β, to assess staining intensity of epithelial cells and stromal cells.Cytoplasmatic staining of canine prostate cells by TIMP-1, TIMP-2 and TGF-β was observed, with TIMP-1 and TIMP-2 being more expressed in premalignant and malignant lesions, while TGF-β was expressed mainly by normal tissue and BPH. Furthermore, there were differences in the expression between epithelial and stromal cells.A próstata canina, além das similaridades com a próstata humana, apresenta grande incidência de afecções. As principais lesões que acometem a próstata são as prostatites, a hiperplasia prostática benigna (HPB), os cistos e o adenocarcinoma, sendo que, recentemente, se tem dado atenção às lesões consideradas pré-malignas, como a neoplasia intraepitelial prostática (PIN) e a atrofia inflamatória proliferativa (PIA), ambas estudadas na glândula humana, e também verificadas na próstata do cão. Para avaliar o desenvolvimento de neoplasias prostáticas a partir das lesões pré-malignas, alguns marcadores imunoistoquímicos são empregados, como os inibidores teciduais de metaloproteinases (TIMP), que apresentam importante função na regulação da ação catalítica das metaloproteinases (MMP), e o fator de crescimento transformador β (TGF-β), que induz a angiogênese e inibe a proliferação celular, sendo considerado um mediador do crescimento prostático. O objetivo deste estudo foi verificar a expressão de TIMP-1, TIMP-2 e TGF-β no tecido prostático canino normal e com lesões proliferativas. Para isso foram colhidas, em exames necroscópicos, 150 próstatas de cães adultos e idosos. O material foi avaliado histologicamente e selecionadas amostras de 54 próstatas com predominância de histomorfologia normal, HPB epitelial, HPB estromal, PIA, PIN e adenocarcinoma, que foram utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à imunoistoquímica com os anticorpos anti-TIMP-1, anti-TIMP-2 e anti-TGF-β, sendo avaliada a intensidade de marcação das células epiteliais e estromais. Verificou-se que há marcação citoplasmática das células prostáticas caninas para TIMP-1, TIMP-2 e TGF-β, sendo as proteínas TIMP-1 e TIMP-2 mais expressas nas lesões proliferativas pré-malignas e malignas, enquanto TGF-β foi expresso principalmente pelo tecido normal e com HPB epitelial e estromal. Ainda, houve diferença de marcação entre células epiteliais e estromais.Made available in DSpace on 2014-07-29T15:07:41Z (GMT). No. of bitstreams: 1 Dissertacao_Denise C Toledo.pdf: 1395118 bytes, checksum: 592dff1a4d2ff80089be7e8691c93893 (MD5) Previous issue date: 2012-03-08application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/3658/Dissertacao_Denise%20C%20Toledo.pdf.jpgporUniversidade Federal de GoiásMestrado em Ciência AnimalUFGBRCiências Agrárias - Veterinariacãodisplasia prostáticaimunoistoquímicaTGF-BTIMP-1TIMP-2dogimmunohistochemistryprostatic dysplasiaTGF-BTIMP-1TIMP-2CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMALExpressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativasExpression of tansforming growth factor B and tissue inhibitor of metalloproteianse 1 and 2 in normal canine prostates and with proliferative lessionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertacao_Denise C Toledo.pdfapplication/pdf1395118http://repositorio.bc.ufg.br/tede/bitstreams/94f66df9-6241-4c8c-9853-c9607a5c7943/download592dff1a4d2ff80089be7e8691c93893MD51THUMBNAILDissertacao_Denise C Toledo.pdf.jpgDissertacao_Denise C Toledo.pdf.jpgGenerated Thumbnailimage/jpeg1992http://repositorio.bc.ufg.br/tede/bitstreams/72ffca4d-950b-4483-8c81-9b26e57f0c39/download3510cd47b5065a7d030ca984cbcc3c1fMD52tde/8832014-07-30 03:07:12.384open.accessoai:repositorio.bc.ufg.br:tde/883http://repositorio.bc.ufg.br/tedeBiblioteca Digital de Teses e Dissertaçõeshttp://repositorio.bc.ufg.br/PUBhttps://repositorio.bc.ufg.br/tede_oai/requesttesesdissertacoes.bc@ufg.br ||tesesdissertacoes.bc@ufg.bropendoar:32082014-07-30T06:07:12Biblioteca Digital de Teses e Dissertações da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
dc.title.alternative.eng.fl_str_mv |
Expression of tansforming growth factor B and tissue inhibitor of metalloproteianse 1 and 2 in normal canine prostates and with proliferative lessions |
title |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
spellingShingle |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas TOLEDO, Denise Caroline cão displasia prostática imunoistoquímica TGF-B TIMP-1 TIMP-2 dog immunohistochemistry prostatic dysplasia TGF-B TIMP-1 TIMP-2 CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
title_short |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
title_full |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
title_fullStr |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
title_full_unstemmed |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
title_sort |
Expressão de fator de crescimento transformador Beta e inibidores teciduais de metaloproteinases 1 e 2 em próstatas caninas normais e com lesões proliferativas |
author |
TOLEDO, Denise Caroline |
author_facet |
TOLEDO, Denise Caroline |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
MOURA, Veridiana Maria Brianezi Dignani de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8773201078957745 |
dc.contributor.advisor-co1.fl_str_mv |
MENEZES, Liliana Borges de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2012543423092393 |
dc.contributor.advisor-co2.fl_str_mv |
BRITO, Luiz Augusto Batista |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/3212353728321020 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0730928874381169 |
dc.contributor.author.fl_str_mv |
TOLEDO, Denise Caroline |
contributor_str_mv |
MOURA, Veridiana Maria Brianezi Dignani de MENEZES, Liliana Borges de BRITO, Luiz Augusto Batista |
dc.subject.por.fl_str_mv |
cão displasia prostática imunoistoquímica TGF-B TIMP-1 TIMP-2 |
topic |
cão displasia prostática imunoistoquímica TGF-B TIMP-1 TIMP-2 dog immunohistochemistry prostatic dysplasia TGF-B TIMP-1 TIMP-2 CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
dc.subject.eng.fl_str_mv |
dog immunohistochemistry prostatic dysplasia TGF-B TIMP-1 TIMP-2 |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
description |
The canine gland has drawn interest for research due to its similarities with the human prostate and the great incidence of lesions. Moreover, the canine prostate shows high incidence of diseases. The main lesions that affect the prostate are prostatitis, benign prostatic hyperplasia (BPH), cysts and adenocarcinoma. Recently attention has been given to lesions considered premalignant such as prostatic intraepithelial neoplasia (PIN) and proliferative inflammatory atrophy (PIA), both studied in the human gland and also found in the canine prostate. In order to evaluate the development of prostate cancer starting as premalignant lesions, some immunohistochemical markers are employed, such as tissue inhibitors of metalloproteinases (TIMP), which have a key role in regulating the catalytic action of metalloproteinases (MMP), and transforming growth factor-β (TGF-β), that induces angiogenesis and inhibits cell proliferation and is considered a mediator of prostate growth. The objective of this study was to investigate the expression of TIMP-1, TIMP-2 and TGF-β in canine normal prostate tissue and with proliferative lesions. For this, 150 adult canine prostates were obtained from postmortem examinations. After microscopic evaluation 54 glands, compatible with normal, epithelial BPH, stromal BPH, PIA, PIN and adenocarcinoma were selected and used to make tissue microarray block (Tissue Microarray - TMA). TMA slides were subjected to immunohistochemistry with anti-TIMP-1, anti-TIMP-2 and anti-TGF-β, to assess staining intensity of epithelial cells and stromal cells.Cytoplasmatic staining of canine prostate cells by TIMP-1, TIMP-2 and TGF-β was observed, with TIMP-1 and TIMP-2 being more expressed in premalignant and malignant lesions, while TGF-β was expressed mainly by normal tissue and BPH. Furthermore, there were differences in the expression between epithelial and stromal cells. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-09-14 |
dc.date.issued.fl_str_mv |
2012-03-08 |
dc.date.accessioned.fl_str_mv |
2014-07-29T15:07:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TOLEDO, Denise Caroline. Expression of tansforming growth factor B and tissue inhibitor of metalloproteianse 1 and 2 in normal canine prostates and with proliferative lessions. 2012. 81 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/883 |
identifier_str_mv |
TOLEDO, Denise Caroline. Expression of tansforming growth factor B and tissue inhibitor of metalloproteianse 1 and 2 in normal canine prostates and with proliferative lessions. 2012. 81 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2012. |
url |
http://repositorio.bc.ufg.br/tede/handle/tde/883 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Goiás |
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Mestrado em Ciência Animal |
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UFG |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciências Agrárias - Veterinaria |
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Universidade Federal de Goiás |
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