Prevalência de parasitos intestinais em gatos errantes em Goiânia – Goiás: ênfase no diagnóstico de Toxoplasma gondii e avaliação da acurácia de técnicas parasitológicas

• Main Author: Rezende, Hanstter Hallison Alves
• Publication Date: 2015
• Format: Master thesis
• Language: por
• Source: Biblioteca Digital de Teses e Dissertações da UFG
• Download full: http://repositorio.bc.ufg.br/tede/handle/tede/4563
• topic: Toxoplasmose; Parasitos intestinais; Gatos errantes; Acurácia diagnóstica; Toxoplasmosis; Intestinal parasites; Stray cats; Accuracy diagnostic; CIENCIAS BIOLOGICAS::PARASITOLOGIA

The domestic cat is an important zoonotic agent for humans, especially as a transmissor of Toxoplasma gondii. The cat is capable of the elimination of millions of oocysts in the environment leading to a massive contamination of these places and leading to a public health issue. Toxoplamosis is a disease with high prevalence throughout the world and the cat is responsible for the parasite’s perpetuation. The evaluation of the accuracy of parasitological diagnostic methods is of vital importance to improve the veterinarian laboratory diagnosis through the use of high sensitivity, specificity and reproducible tests. The aim of this study was to verify the prevalence of intestinal parasites from stray cats in Goiania-GO, to verify the seroprevalence of anti-T. gondii antibodies through the modified aglutination test (MAT) and to perform the evaluation of the accuracy of the parasitological tests applied. Therefore 155 samples of feces and 50 blood samples from stray cats from the Zoonosis Center of Goiania were collected during the year of 2012. The feces samples were processed by the Willis (gold standard), Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) techniques. The MAT was performed in the blood samples. The accuracy was performed through the determination of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and kappa index (k). The intestinal parasites prevalence in the feces from stray cats was 71.68% (115/154). The most prevalent helminth was Ancylostomatids (83.48%, 96/115) and the most prevalent protozoan in monoparasitism was Cystoisospora sp (7.83%, 9/115). T. gondii presented high prevalence (18.27%, 21/115) in fecal samples, 64% in blood samples (32/50) while 36% seronegative (18/50). The accuracy showed that the HJPL and Sheather techniques presented association to the golden standard for the intestinal parasites diagnosis. For the specific diagnosis of T. gondii oocysts the Faust techniques was the one with high accuracy in comparasion to the golden standard, with we emphasize the importance of using two parasitological techniques for the diagnosis of intestinal parasites