Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência e Agrotecnologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008 |
Resumo: | A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats. |
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Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesisAberrant crypt focibrazilian savannahdimethylhydrazineimmunohistochemistryoxidative stressA. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.Editora da UFLA2013-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008Ciência e Agrotecnologia v.37 n.4 2013reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542013000400008info:eu-repo/semantics/openAccessVenâncio,Vinícius PaulaFerreira,Eric BatistaBrigagão,Maísa Ribeiro Pereira LimaPaula,Fernanda Borges de AraújoBarbisan,Luis FernandoAzevedo,Lucianaeng2013-09-11T00:00:00Zoai:scielo:S1413-70542013000400008Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:31:17.392306Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
spellingShingle |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis Venâncio,Vinícius Paula Aberrant crypt foci brazilian savannah dimethylhydrazine immunohistochemistry oxidative stress |
title_short |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title_full |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title_fullStr |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title_full_unstemmed |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
title_sort |
Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis |
author |
Venâncio,Vinícius Paula |
author_facet |
Venâncio,Vinícius Paula Ferreira,Eric Batista Brigagão,Maísa Ribeiro Pereira Lima Paula,Fernanda Borges de Araújo Barbisan,Luis Fernando Azevedo,Luciana |
author_role |
author |
author2 |
Ferreira,Eric Batista Brigagão,Maísa Ribeiro Pereira Lima Paula,Fernanda Borges de Araújo Barbisan,Luis Fernando Azevedo,Luciana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Venâncio,Vinícius Paula Ferreira,Eric Batista Brigagão,Maísa Ribeiro Pereira Lima Paula,Fernanda Borges de Araújo Barbisan,Luis Fernando Azevedo,Luciana |
dc.subject.por.fl_str_mv |
Aberrant crypt foci brazilian savannah dimethylhydrazine immunohistochemistry oxidative stress |
topic |
Aberrant crypt foci brazilian savannah dimethylhydrazine immunohistochemistry oxidative stress |
description |
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-70542013000400008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da UFLA |
publisher.none.fl_str_mv |
Editora da UFLA |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.37 n.4 2013 reponame:Ciência e Agrotecnologia (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Ciência e Agrotecnologia (Online) |
collection |
Ciência e Agrotecnologia (Online) |
repository.name.fl_str_mv |
Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
||renpaiva@dbi.ufla.br|| editora@editora.ufla.br |
_version_ |
1799874969212026880 |