Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Coffee Science (Online) |
Texto Completo: | https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071 |
Resumo: | The objective of this study was to evaluate the effect of resistance inducers, plant extract-based formulations and fungicide over the protection of coffee seedlings in controlling Cercospora coffeicola. In addition, the objective was to select alternative treatments that highlight in control of Cercospora leaf spot and study its effect as resistance inducer through the defense gene expression analysis. The treatments applied were: Greenforce CuCa, Greenforce KP, ET64-DT, which are plant extracts-based formulations (are under patent secrecy - PI 0603575-2), mineral fertilizer, acibenzolar-S-methyl (ASM) and, as control, the epoxiconazole + pyraclostrobin fungicide. We conducted weekly evaluations of incidence of cercosporiose to calculate the area under the curve of disease incidence progress (AUCDIP). After analysis of this experiment, the two best alternative treatments to evaluate the resistance-inducing potential of them were chosen. For this, a second experiment was carried out in order to analyze the expression of genes that are translated into the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes. The application of epoxiconazole + pyraclostrobin fungicide resulted in more effective control of the incidence of the disease, differing from the other treatments tested. The Greenforce Cuca and ASM, 0.05 g.L-1, products resulted in a reduction of AUCDIP of cercosporiose by 38 and 35%, respectively, differing from the other treatments. In the analysis of gene response expression to induce, it was observed that the highest expression of the genes encoding the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes occurred at 24 hours after application of ASM and 48 hours for Greenforce Cuca, no difference from one treatment to the other in these periods. Plants were sprayed with Greenforce Cuca showed a higher expression of the lipoxygenase gene at 72 hours after application of this product, differed from the ASM. With this work, we found that Greenforce Cuca and ASM products control Cercospora leaf spot through the induction of defense genes in coffee. |
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Resistance inductors in control of coffee cercosporiose: analysis of genes related to defenseIndutores de resistência no controle da cercosporiose do cafeeiro: análise de genes relacionados à defesaResistance inductionoxygen reactive speciesPR-proteinlipid oxidationIndução de resistênciaespécies reativas de oxigênioPR- proteínaoxidação de lipídeosThe objective of this study was to evaluate the effect of resistance inducers, plant extract-based formulations and fungicide over the protection of coffee seedlings in controlling Cercospora coffeicola. In addition, the objective was to select alternative treatments that highlight in control of Cercospora leaf spot and study its effect as resistance inducer through the defense gene expression analysis. The treatments applied were: Greenforce CuCa, Greenforce KP, ET64-DT, which are plant extracts-based formulations (are under patent secrecy - PI 0603575-2), mineral fertilizer, acibenzolar-S-methyl (ASM) and, as control, the epoxiconazole + pyraclostrobin fungicide. We conducted weekly evaluations of incidence of cercosporiose to calculate the area under the curve of disease incidence progress (AUCDIP). After analysis of this experiment, the two best alternative treatments to evaluate the resistance-inducing potential of them were chosen. For this, a second experiment was carried out in order to analyze the expression of genes that are translated into the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes. The application of epoxiconazole + pyraclostrobin fungicide resulted in more effective control of the incidence of the disease, differing from the other treatments tested. The Greenforce Cuca and ASM, 0.05 g.L-1, products resulted in a reduction of AUCDIP of cercosporiose by 38 and 35%, respectively, differing from the other treatments. In the analysis of gene response expression to induce, it was observed that the highest expression of the genes encoding the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes occurred at 24 hours after application of ASM and 48 hours for Greenforce Cuca, no difference from one treatment to the other in these periods. Plants were sprayed with Greenforce Cuca showed a higher expression of the lipoxygenase gene at 72 hours after application of this product, differed from the ASM. With this work, we found that Greenforce Cuca and ASM products control Cercospora leaf spot through the induction of defense genes in coffee.Objetivou-se, neste trabalho, avaliar o efeito de indutores de resistência, formulações à base de extratos vegetais e fungicida na proteção de mudas de cafeeiro no controle de Cercospora coffeicola. Além disso, objetivou-se selecionar os tratamentos alternativos que se destacaram no controle da cercosporiose e estudar seu efeito como indutor de resistência, por meio da análise da expressão de genes de defesa. Os tratamentos aplicados foram: Greenforce CuCa, Greenforce KP e ET64-DT, que são formulações à base de extratos vegetais (estão sob sigilo de patente - PI 0603575-2), fertilizante mineral, acibenzolar-S-metil (ASM), e como controle, o fungicida epoxiconazol + piraclostrobina. Foram realizadas avaliações semanais de incidência da cercosporiose para cálculo da área abaixo da curva de progresso da incidência (AACPI) da doença. Após análise deste experimento, foram escolhidos os dois melhores tratamentos alternativos para avaliar o potencial de indução de resistência dos mesmos. Para isso, foi instalado um segundo experimento para analisar a expressão de genes que são traduzidos nas enzimas lipoxigenase, catalase, glutationa peroxidase e quitinase. A aplicação do fungicida epoxiconazol + piraclostrobina resultou em controle mais efetivo da incidência da doença, diferindo dos demais tratamentos testados. Os produtos Greenforce CuCa e ASM, na dose 0,05 g.L-1, proporcionaram redução de 38 e 35%, respectivamente, da AACPI da cercosporiose, diferindo dos demais tratamentos. Na análise da expressão gênica da resposta aos indutores, observou-se que a maior expressão dos genes que codificam as enzimas lipoxigenase, catalase, glutationa peroxidase e quitinase ocorreu às 24 horas, após aplicação do ASM, e às 48 horas para o Greenforce CuCa, havendo diferença de um tratamento para o outro nestes períodos. Plantas que foram pulverizadas com Greenforce CuCa apresentaram, também, maior expressão do gene da lipoxigenase às 72 horas após a aplicação deste produto, diferindo do ASM. Com este trabalho, foi possível verificar que os produtos Greenforce CuCa e ASM controlam a cercosporiose por meio da indução de genes de defesa em cafeeiro.Editora UFLA2016-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/mswordapplication/mswordhttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071Coffee Science - ISSN 1984-3909; Vol. 11 No. 3 (2016); 308 - 317Coffee Science; Vol. 11 Núm. 3 (2016); 308 - 317Coffee Science; v. 11 n. 3 (2016); 308 - 3171984-3909reponame:Coffee Science (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/pdf_1071https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/1571https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/1572Copyright (c) 2016 Coffee Science - ISSN 1984-3909https://creativecommons.org/info:eu-repo/semantics/openAccessGUIMARÃES, SANDRA ELIZARESENDE, MÁRIO LÚCIODOS SANTOS, DEILAMONTEIRO, ANA CRISTINAVASCONCELOS, VICTOR AUGUSTOJÚNIOR, MANOEL BATISTA2016-10-18T14:32:44Zoai:coffeescience.ufla.br:article/1071Revistahttps://coffeescience.ufla.br/index.php/CoffeesciencePUBhttps://coffeescience.ufla.br/index.php/Coffeescience/oaicoffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com1984-39091809-6875opendoar:2016-10-18T14:32:44Coffee Science (Online) - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense Indutores de resistência no controle da cercosporiose do cafeeiro: análise de genes relacionados à defesa |
title |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
spellingShingle |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense GUIMARÃES, SANDRA ELIZA Resistance induction oxygen reactive species PR-protein lipid oxidation Indução de resistência espécies reativas de oxigênio PR- proteína oxidação de lipídeos |
title_short |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
title_full |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
title_fullStr |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
title_full_unstemmed |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
title_sort |
Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense |
author |
GUIMARÃES, SANDRA ELIZA |
author_facet |
GUIMARÃES, SANDRA ELIZA RESENDE, MÁRIO LÚCIO DOS SANTOS, DEILA MONTEIRO, ANA CRISTINA VASCONCELOS, VICTOR AUGUSTO JÚNIOR, MANOEL BATISTA |
author_role |
author |
author2 |
RESENDE, MÁRIO LÚCIO DOS SANTOS, DEILA MONTEIRO, ANA CRISTINA VASCONCELOS, VICTOR AUGUSTO JÚNIOR, MANOEL BATISTA |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
GUIMARÃES, SANDRA ELIZA RESENDE, MÁRIO LÚCIO DOS SANTOS, DEILA MONTEIRO, ANA CRISTINA VASCONCELOS, VICTOR AUGUSTO JÚNIOR, MANOEL BATISTA |
dc.subject.por.fl_str_mv |
Resistance induction oxygen reactive species PR-protein lipid oxidation Indução de resistência espécies reativas de oxigênio PR- proteína oxidação de lipídeos |
topic |
Resistance induction oxygen reactive species PR-protein lipid oxidation Indução de resistência espécies reativas de oxigênio PR- proteína oxidação de lipídeos |
description |
The objective of this study was to evaluate the effect of resistance inducers, plant extract-based formulations and fungicide over the protection of coffee seedlings in controlling Cercospora coffeicola. In addition, the objective was to select alternative treatments that highlight in control of Cercospora leaf spot and study its effect as resistance inducer through the defense gene expression analysis. The treatments applied were: Greenforce CuCa, Greenforce KP, ET64-DT, which are plant extracts-based formulations (are under patent secrecy - PI 0603575-2), mineral fertilizer, acibenzolar-S-methyl (ASM) and, as control, the epoxiconazole + pyraclostrobin fungicide. We conducted weekly evaluations of incidence of cercosporiose to calculate the area under the curve of disease incidence progress (AUCDIP). After analysis of this experiment, the two best alternative treatments to evaluate the resistance-inducing potential of them were chosen. For this, a second experiment was carried out in order to analyze the expression of genes that are translated into the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes. The application of epoxiconazole + pyraclostrobin fungicide resulted in more effective control of the incidence of the disease, differing from the other treatments tested. The Greenforce Cuca and ASM, 0.05 g.L-1, products resulted in a reduction of AUCDIP of cercosporiose by 38 and 35%, respectively, differing from the other treatments. In the analysis of gene response expression to induce, it was observed that the highest expression of the genes encoding the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes occurred at 24 hours after application of ASM and 48 hours for Greenforce Cuca, no difference from one treatment to the other in these periods. Plants were sprayed with Greenforce Cuca showed a higher expression of the lipoxygenase gene at 72 hours after application of this product, differed from the ASM. With this work, we found that Greenforce Cuca and ASM products control Cercospora leaf spot through the induction of defense genes in coffee. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071 |
url |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/pdf_1071 https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/1571 https://coffeescience.ufla.br/index.php/Coffeescience/article/view/1071/1572 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Coffee Science - ISSN 1984-3909 https://creativecommons.org/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Coffee Science - ISSN 1984-3909 https://creativecommons.org/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/msword application/msword |
dc.publisher.none.fl_str_mv |
Editora UFLA |
publisher.none.fl_str_mv |
Editora UFLA |
dc.source.none.fl_str_mv |
Coffee Science - ISSN 1984-3909; Vol. 11 No. 3 (2016); 308 - 317 Coffee Science; Vol. 11 Núm. 3 (2016); 308 - 317 Coffee Science; v. 11 n. 3 (2016); 308 - 317 1984-3909 reponame:Coffee Science (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Coffee Science (Online) |
collection |
Coffee Science (Online) |
repository.name.fl_str_mv |
Coffee Science (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
coffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com |
_version_ |
1789440322046001152 |