Physiological quality and amylase enzyme expression in maize seeds
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/1481 |
Resumo: | The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines. |
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Physiological quality and amylase enzyme expression in maize seedsQualidade fisiológica e expressão das enzimas amilases em sementes de milhoMilhoSeeds - QualityqRT-PCRSementes - qualidadeCornThe physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines.A qualidade fisiológica de sementes de milho é influenciada pelo genótipo. Assim, o estudo da expressão de genes associados a essa característica é importante no processo de seleção de genótipos em programas de melhoramento. O objetivo neste trabalho foi estudar a expressão das enzimas amilases associadas à qualidade fisiológica de sementes de milho, de diferentes genótipos e tamanhos de sementes. Objetivou-se ainda avaliar a expressão dessas enzimas em sementes secas e embebidas. O experimento foi conduzido em área experimental e no Laboratório Central de Sementes do Departamento de Agricultura da Universidade Federal de Lavras. Foram utilizadas sementes de quatro linhagens de milho, classificadas em dois tamanhos. A qualidade fisiológica das sementes foi avaliada por meio dos testes de germinação, emergência de plântulas, índice de velocidade de emergência de plântulas e envelhecimento artificial. A expressão da enzima α-amilase foi avaliada pela técnica de eletroforese e a expressão dos genes alpha amylase B73, alpha amylase (LOC542522) e beta amylase 5 (amyb5), foi estudada pela técnica de qRT-PCR em sementes secas e embebidas das linhagens. Há expressão diferenciada das enzimas amilases em sementes de linhagens com diferentes níveis de qualidade fisiológica. Maior expressão das enzimas amilases é observada em sementes de milho de menor tamanho e embebidas.Editora da Universidade Federal de Lavras2013-12-06T16:44:50Z2013-12-06T16:44:50Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, G. E. et al. Physiological quality and amylase enzyme expression in maize seeds. Ciência e Agrotecnologia, Lavras, v. 37, n. 1, p. 40-48, jan./fev. 2013.http://repositorio.ufla.br/jspui/handle/1/1481Ciência e Agrotecnologiareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAOliveira, Gustavo EvangelistaVon Pinho, Renzo GarciaAndrade, Thaís deVon Pinho, Édila Vilela de ResendeSantos, Custódio Donizete dosVeiga, Adriano Dellyinfo:eu-repo/semantics/openAccesspor2013-12-06T16:54:46Zoai:localhost:1/1481Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2013-12-06T16:54:46Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Physiological quality and amylase enzyme expression in maize seeds Qualidade fisiológica e expressão das enzimas amilases em sementes de milho |
title |
Physiological quality and amylase enzyme expression in maize seeds |
spellingShingle |
Physiological quality and amylase enzyme expression in maize seeds Oliveira, Gustavo Evangelista Milho Seeds - Quality qRT-PCR Sementes - qualidade Corn |
title_short |
Physiological quality and amylase enzyme expression in maize seeds |
title_full |
Physiological quality and amylase enzyme expression in maize seeds |
title_fullStr |
Physiological quality and amylase enzyme expression in maize seeds |
title_full_unstemmed |
Physiological quality and amylase enzyme expression in maize seeds |
title_sort |
Physiological quality and amylase enzyme expression in maize seeds |
author |
Oliveira, Gustavo Evangelista |
author_facet |
Oliveira, Gustavo Evangelista Von Pinho, Renzo Garcia Andrade, Thaís de Von Pinho, Édila Vilela de Resende Santos, Custódio Donizete dos Veiga, Adriano Delly |
author_role |
author |
author2 |
Von Pinho, Renzo Garcia Andrade, Thaís de Von Pinho, Édila Vilela de Resende Santos, Custódio Donizete dos Veiga, Adriano Delly |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Gustavo Evangelista Von Pinho, Renzo Garcia Andrade, Thaís de Von Pinho, Édila Vilela de Resende Santos, Custódio Donizete dos Veiga, Adriano Delly |
dc.subject.por.fl_str_mv |
Milho Seeds - Quality qRT-PCR Sementes - qualidade Corn |
topic |
Milho Seeds - Quality qRT-PCR Sementes - qualidade Corn |
description |
The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-06T16:44:50Z 2013-12-06T16:44:50Z 2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, G. E. et al. Physiological quality and amylase enzyme expression in maize seeds. Ciência e Agrotecnologia, Lavras, v. 37, n. 1, p. 40-48, jan./fev. 2013. http://repositorio.ufla.br/jspui/handle/1/1481 |
identifier_str_mv |
OLIVEIRA, G. E. et al. Physiological quality and amylase enzyme expression in maize seeds. Ciência e Agrotecnologia, Lavras, v. 37, n. 1, p. 40-48, jan./fev. 2013. |
url |
http://repositorio.ufla.br/jspui/handle/1/1481 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784549952519667712 |