Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses

Detalhes bibliográficos
Autor(a) principal: Pádua, Marlúcia Souza
Data de Publicação: 2013
Outros Autores: Paiva, Luciano Vilela, Alves, Eduardo, Labory, Claudia Regina Gontijo, Stein, Vanessa Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28543
Resumo: Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm
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spelling Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic massesEmbryogenic potentialGrowth regulatorsTissue cultureTransmission electron microscopyOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palmO dendezeiro é uma das oleoginosas mais importante economicamente, mas a expansão das plantações tem sido limitada pela disponibilidade de mudas, como a propagação convencional é através de sementes, que têm baixas taxas de germinação. Uma possível solução para produção em larga escala é por meio da embriogênese somática. O objetivo deste trabalho foi avaliar os efeitos das auxinas 2,4-D e picloram na indução de massas pró-embriogênicas em explantes foliares de Elaeis guineenesis híbrido Tenera e caracterizá-las, considerando as características embriogênicas, com analises citoquímicas e ultraestruturais. Para indução das massas pró- embriogênicas, fragmentos foliares de plântulas in vitro foram inoculados em meio de cultivo Y3 suplementado com 2,4-D ou picloram em diferentes concentrações (0,0; 1,0; 3,0; 6,0 e 9,0 mg.L–1). Após 90 dias foram avaliadas presença e ausência das massas celulares. Ambos reguladores de crescimento foram eficientes na indução destas massas, independentemente, das concentrações utilizadas. Como as massas celulares não eram homogêneas, estas foram classificadas quanto à cor e ao formato em quatro tipos: TIPO 1 – translúcido e alongado, TIPO 2 – translúcido e aquoso, TIPO 3 – bege e globular e TIPO 4 - branco e globular). De acordo com as características anatômicas e ultraestruturais os as massas celulares dos TIPOS 2, 3 e 4, foram consideradas com maior potencial embriogênico e portanto podem ser as mais promissoras para a propagação vegetativa em larga escala para o dendezeiro.Academia Brasileira de Ciências2018-02-06T15:35:56Z2018-02-06T15:35:56Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013.http://repositorio.ufla.br/jspui/handle/1/28543Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessPádua, Marlúcia SouzaPaiva, Luciano VilelaAlves, EduardoLabory, Claudia Regina GontijoStein, Vanessa Cristinaeng2018-02-06T15:35:56Zoai:localhost:1/28543Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-02-06T15:35:56Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
title Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
spellingShingle Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
Pádua, Marlúcia Souza
Embryogenic potential
Growth regulators
Tissue culture
Transmission electron microscopy
title_short Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
title_full Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
title_fullStr Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
title_full_unstemmed Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
title_sort Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
author Pádua, Marlúcia Souza
author_facet Pádua, Marlúcia Souza
Paiva, Luciano Vilela
Alves, Eduardo
Labory, Claudia Regina Gontijo
Stein, Vanessa Cristina
author_role author
author2 Paiva, Luciano Vilela
Alves, Eduardo
Labory, Claudia Regina Gontijo
Stein, Vanessa Cristina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pádua, Marlúcia Souza
Paiva, Luciano Vilela
Alves, Eduardo
Labory, Claudia Regina Gontijo
Stein, Vanessa Cristina
dc.subject.por.fl_str_mv Embryogenic potential
Growth regulators
Tissue culture
Transmission electron microscopy
topic Embryogenic potential
Growth regulators
Tissue culture
Transmission electron microscopy
description Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm
publishDate 2013
dc.date.none.fl_str_mv 2013
2018-02-06T15:35:56Z
2018-02-06T15:35:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013.
http://repositorio.ufla.br/jspui/handle/1/28543
identifier_str_mv PÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013.
url http://repositorio.ufla.br/jspui/handle/1/28543
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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