Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor

Detalhes bibliográficos
Autor(a) principal: Souza, Denys Matheus Santana Costa
Data de Publicação: 2022
Outros Autores: Martins, Andressa Rosa, Fernandes, Sérgio Bruno, Santos, Juscelina Arcanjo dos, Brondani, Gilvano Ebling
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/50930
Resumo: Micropropagation is one technology to propagate endemic species of the Ferruginous Rupestrian Grasslands when in vitro genetic conservation is sought. The present study aimed to assess the breaking of dormancy, in vitro establishment, multiplication, elongation, rooting, and acclimatization of Mimosa calodendron from culture in a temporary immersion bioreactor system. The seeds used for the experiments were from plants originating from the Ferruginous Rupestrian Grasslands. The percentage of contamination, oxidation, unresponsive seeds, germination, number of buds per explant, shoot length, senescence, percentage of adventitious rooting, and acclimatization were assessed. The breaking of dormancy was most successful by mechanical scarification (80% germination). Immersion in sodium hypochlorite for 5 minutes was the most efficient treatment for in vitro establishment (90%). For the in vitro multiplication and elongation phase, the use of liquid culture medium from cultivation in a temporary immersion bioreactor was the most suitable for the characteristics number of buds per explant (2.55 buds), vigor (1.1), oxidation (1.3) and senescence (1.3) according to the score’s scale. Regardless of the in vitro cultivation method, the percentages of rooting and acclimatization were satisfactory, and it was possible to obtain complete plants in 190 days.
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spelling Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactorProdução de mudas de Mimosacalodendron Mart. ex Benth. em biorreator de imersão temporáriaIn vitro propagationFerruginous rupestrian grasslandsEndemic speciesPropagação in vitroCampo rupestre ferruginosoEspécie endêmicaMicropropagation is one technology to propagate endemic species of the Ferruginous Rupestrian Grasslands when in vitro genetic conservation is sought. The present study aimed to assess the breaking of dormancy, in vitro establishment, multiplication, elongation, rooting, and acclimatization of Mimosa calodendron from culture in a temporary immersion bioreactor system. The seeds used for the experiments were from plants originating from the Ferruginous Rupestrian Grasslands. The percentage of contamination, oxidation, unresponsive seeds, germination, number of buds per explant, shoot length, senescence, percentage of adventitious rooting, and acclimatization were assessed. The breaking of dormancy was most successful by mechanical scarification (80% germination). Immersion in sodium hypochlorite for 5 minutes was the most efficient treatment for in vitro establishment (90%). For the in vitro multiplication and elongation phase, the use of liquid culture medium from cultivation in a temporary immersion bioreactor was the most suitable for the characteristics number of buds per explant (2.55 buds), vigor (1.1), oxidation (1.3) and senescence (1.3) according to the score’s scale. Regardless of the in vitro cultivation method, the percentages of rooting and acclimatization were satisfactory, and it was possible to obtain complete plants in 190 days.A micropropagação é uma alternativa para a propagação de espécies endêmicas do Campo Rupestre Ferruginoso, quando se busca a conservação genética in vitro. O trabalho teve como objetivo avaliar a superação de dormência, estabelecimento in vitro, multiplicação, alongamento, enraizamento e aclimatização de Mimosa calodendron a partir do cultivo em sistema de biorreator de imersão temporária (BIT). As sementes utilizadas para os experimentos foram provenientes de plantas oriundas do Campo Rupestre Ferruginoso. A porcentagem de contaminação, oxidação, sementes não responsivas, germinação, número de gemas por explante, comprimento de brotos, senescência, porcentagem de enraizamento e aclimatização foram avaliados. A superação de dormência por escarificação mecânica (80% de germinação) foi a mais adequada para a superação de dormência. A imersão em hipoclorito de sódio por 5 minutos foi o tratamento mais eficiente para o estabelecimento in vitro (90%). Para a fase de multiplicação e alongamento in vitro, o uso do meio de cultivo líquido a partir do cultivo em biorreator de imersão temporária foi o mais adequado para as características número de gemas por explante (2,55 gemas), vigor (1,1), oxidação (1,3) e senescência (1,3) de acordo à escala de notas. Independentemente do método de cultivo in vitro, a porcentagem de enraizamento e aclimatização foram satisfatórios, sendo possível obter plantas completas em 190 dias.Universidade Federal de Mato Grosso2022-08-11T19:19:09Z2022-08-11T19:19:09Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, D. M. S. C. et al. Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor. Nativa, Sinop, v. 10, n. 1, 2022.http://repositorio.ufla.br/jspui/handle/1/50930Nativareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessSouza, Denys Matheus Santana CostaMartins, Andressa RosaFernandes, Sérgio BrunoSantos, Juscelina Arcanjo dosBrondani, Gilvano Eblingeng2022-08-11T19:19:10Zoai:localhost:1/50930Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-08-11T19:19:10Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
Produção de mudas de Mimosacalodendron Mart. ex Benth. em biorreator de imersão temporária
title Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
spellingShingle Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
Souza, Denys Matheus Santana Costa
In vitro propagation
Ferruginous rupestrian grasslands
Endemic species
Propagação in vitro
Campo rupestre ferruginoso
Espécie endêmica
title_short Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
title_full Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
title_fullStr Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
title_full_unstemmed Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
title_sort Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor
author Souza, Denys Matheus Santana Costa
author_facet Souza, Denys Matheus Santana Costa
Martins, Andressa Rosa
Fernandes, Sérgio Bruno
Santos, Juscelina Arcanjo dos
Brondani, Gilvano Ebling
author_role author
author2 Martins, Andressa Rosa
Fernandes, Sérgio Bruno
Santos, Juscelina Arcanjo dos
Brondani, Gilvano Ebling
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Denys Matheus Santana Costa
Martins, Andressa Rosa
Fernandes, Sérgio Bruno
Santos, Juscelina Arcanjo dos
Brondani, Gilvano Ebling
dc.subject.por.fl_str_mv In vitro propagation
Ferruginous rupestrian grasslands
Endemic species
Propagação in vitro
Campo rupestre ferruginoso
Espécie endêmica
topic In vitro propagation
Ferruginous rupestrian grasslands
Endemic species
Propagação in vitro
Campo rupestre ferruginoso
Espécie endêmica
description Micropropagation is one technology to propagate endemic species of the Ferruginous Rupestrian Grasslands when in vitro genetic conservation is sought. The present study aimed to assess the breaking of dormancy, in vitro establishment, multiplication, elongation, rooting, and acclimatization of Mimosa calodendron from culture in a temporary immersion bioreactor system. The seeds used for the experiments were from plants originating from the Ferruginous Rupestrian Grasslands. The percentage of contamination, oxidation, unresponsive seeds, germination, number of buds per explant, shoot length, senescence, percentage of adventitious rooting, and acclimatization were assessed. The breaking of dormancy was most successful by mechanical scarification (80% germination). Immersion in sodium hypochlorite for 5 minutes was the most efficient treatment for in vitro establishment (90%). For the in vitro multiplication and elongation phase, the use of liquid culture medium from cultivation in a temporary immersion bioreactor was the most suitable for the characteristics number of buds per explant (2.55 buds), vigor (1.1), oxidation (1.3) and senescence (1.3) according to the score’s scale. Regardless of the in vitro cultivation method, the percentages of rooting and acclimatization were satisfactory, and it was possible to obtain complete plants in 190 days.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-11T19:19:09Z
2022-08-11T19:19:09Z
2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, D. M. S. C. et al. Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor. Nativa, Sinop, v. 10, n. 1, 2022.
http://repositorio.ufla.br/jspui/handle/1/50930
identifier_str_mv SOUZA, D. M. S. C. et al. Seedling production of Mimosa calodendron Mart. ex Benth. in a temporary immersion bioreactor. Nativa, Sinop, v. 10, n. 1, 2022.
url http://repositorio.ufla.br/jspui/handle/1/50930
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
dc.source.none.fl_str_mv Nativa
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
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