Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.academicjournals.org/article/article1379332438_Silva%20et%20al.pdf http://repositorio.ufla.br/jspui/handle/1/12461 |
Resumo: | Lectins have various biotechnological applications including their use in agriculture as antimicrobials and pesticides. Lectin from manioc leaf powder has been extracted, partially purified and evaluated in terms of its haemagglutination activity and fungicidal properties against Fusarium oxysporum. Ground, dried and the entire leaves of Manihot esculenta (Cacao cultivar) were extracted with distilled water and the protein extract was concentrated by precipitation in 80% saturated ammonium sulphate solution. The concentrated protein solution was partially purified on a Sepharose column and the unbound protein fraction was submitted to native and denaturing polyacrylamide gel electrophoresis. Lectin from manioc leaf powder exhibited a haemagglutination (HU) activity of 0.5 HU/100 μl and was stable over a wide temperatures range (up to 70°C). Four protein bands (of 13, 58, 61, 64 kDa) were identified in the unbound protein fraction obtained by column chromatography, and the presence of a lectin was demonstrated by the determination of an HA value of 0.5 HU/100 μl. The concentrated protein solution was not, however, active against F. oxysporum at concentrations up to 100 μg/ml. |
id |
UFLA_78e457672656605f1934b7bdcb4c5449 |
---|---|
oai_identifier_str |
oai:localhost:1/12461 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activityManihot esculentaEuphorbiaceaeManiocFungicidal activityHemagglutination activityLectinLectins have various biotechnological applications including their use in agriculture as antimicrobials and pesticides. Lectin from manioc leaf powder has been extracted, partially purified and evaluated in terms of its haemagglutination activity and fungicidal properties against Fusarium oxysporum. Ground, dried and the entire leaves of Manihot esculenta (Cacao cultivar) were extracted with distilled water and the protein extract was concentrated by precipitation in 80% saturated ammonium sulphate solution. The concentrated protein solution was partially purified on a Sepharose column and the unbound protein fraction was submitted to native and denaturing polyacrylamide gel electrophoresis. Lectin from manioc leaf powder exhibited a haemagglutination (HU) activity of 0.5 HU/100 μl and was stable over a wide temperatures range (up to 70°C). Four protein bands (of 13, 58, 61, 64 kDa) were identified in the unbound protein fraction obtained by column chromatography, and the presence of a lectin was demonstrated by the determination of an HA value of 0.5 HU/100 μl. The concentrated protein solution was not, however, active against F. oxysporum at concentrations up to 100 μg/ml.Academic Journals2017-03-10T19:36:40Z2017-03-10T19:36:40Z2010-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSILVA, M. C. et al. Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity. Journal of Agricultural Biotechnology and Sustainable Development, [S. l.], v. 2, n. 8, p. 136-141, Sept. 2010.http://www.academicjournals.org/article/article1379332438_Silva%20et%20al.pdfhttp://repositorio.ufla.br/jspui/handle/1/12461Journal of Agricultural Biotechnology and Sustainable Developmentreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASilva, Maria CristinaCorrêa, Angelita DuarteSantos, Custódio Donizete dosMarcos, Flávia Cristina AlmeidaAbreu, Celeste Maria Patto deinfo:eu-repo/semantics/openAccesspor2017-03-10T19:37:53Zoai:localhost:1/12461Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-03-10T19:37:53Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
title |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
spellingShingle |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity Silva, Maria Cristina Manihot esculenta Euphorbiaceae Manioc Fungicidal activity Hemagglutination activity Lectin |
title_short |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
title_full |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
title_fullStr |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
title_full_unstemmed |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
title_sort |
Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity |
author |
Silva, Maria Cristina |
author_facet |
Silva, Maria Cristina Corrêa, Angelita Duarte Santos, Custódio Donizete dos Marcos, Flávia Cristina Almeida Abreu, Celeste Maria Patto de |
author_role |
author |
author2 |
Corrêa, Angelita Duarte Santos, Custódio Donizete dos Marcos, Flávia Cristina Almeida Abreu, Celeste Maria Patto de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Maria Cristina Corrêa, Angelita Duarte Santos, Custódio Donizete dos Marcos, Flávia Cristina Almeida Abreu, Celeste Maria Patto de |
dc.subject.por.fl_str_mv |
Manihot esculenta Euphorbiaceae Manioc Fungicidal activity Hemagglutination activity Lectin |
topic |
Manihot esculenta Euphorbiaceae Manioc Fungicidal activity Hemagglutination activity Lectin |
description |
Lectins have various biotechnological applications including their use in agriculture as antimicrobials and pesticides. Lectin from manioc leaf powder has been extracted, partially purified and evaluated in terms of its haemagglutination activity and fungicidal properties against Fusarium oxysporum. Ground, dried and the entire leaves of Manihot esculenta (Cacao cultivar) were extracted with distilled water and the protein extract was concentrated by precipitation in 80% saturated ammonium sulphate solution. The concentrated protein solution was partially purified on a Sepharose column and the unbound protein fraction was submitted to native and denaturing polyacrylamide gel electrophoresis. Lectin from manioc leaf powder exhibited a haemagglutination (HU) activity of 0.5 HU/100 μl and was stable over a wide temperatures range (up to 70°C). Four protein bands (of 13, 58, 61, 64 kDa) were identified in the unbound protein fraction obtained by column chromatography, and the presence of a lectin was demonstrated by the determination of an HA value of 0.5 HU/100 μl. The concentrated protein solution was not, however, active against F. oxysporum at concentrations up to 100 μg/ml. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09 2017-03-10T19:36:40Z 2017-03-10T19:36:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, M. C. et al. Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity. Journal of Agricultural Biotechnology and Sustainable Development, [S. l.], v. 2, n. 8, p. 136-141, Sept. 2010. http://www.academicjournals.org/article/article1379332438_Silva%20et%20al.pdf http://repositorio.ufla.br/jspui/handle/1/12461 |
identifier_str_mv |
SILVA, M. C. et al. Partial purification of leaf lectin from Manihot esculenta and screening its fungicidal activity. Journal of Agricultural Biotechnology and Sustainable Development, [S. l.], v. 2, n. 8, p. 136-141, Sept. 2010. |
url |
http://www.academicjournals.org/article/article1379332438_Silva%20et%20al.pdf http://repositorio.ufla.br/jspui/handle/1/12461 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Academic Journals |
publisher.none.fl_str_mv |
Academic Journals |
dc.source.none.fl_str_mv |
Journal of Agricultural Biotechnology and Sustainable Development reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784550102406266880 |