Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator

Detalhes bibliográficos
Autor(a) principal: Paula, Daniella Aparecida de Jesus
Data de Publicação: 2014
Outros Autores: Machado, Mônica Rodrigues Ferreira, Murgas, Luis David Solis, Rabelo, Denilson, Zangerônimo, Márcio Gilberto, Reis Neto, Rafael Vilhena, Andrade, Estefânia de Souza, Andrade, Eliane Aparecida de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39221
Resumo: This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo diameter and embryo volume comparing the treatments versus control group (water) (p<0.05), this results can help in future cryopreservation protocols. Although the temperature and oxygenation differed between the usual and experimental incubators (p<0.05), the results showed a high fertilization rate (79.6 ± 13.2%) for experimental incubators (p<0.05) which is sufficient for the maintenance of embryos in a cryoprotective environment and effectively allows experimentation for long periods with cryoprotectant substances. Cryopreservation of fish embryos has not been accomplished yet and new approaches are required for understanding the permeability of teleost embryos, especially in Brazilian native species.
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spelling Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubatorFish - ReproductionFertilizationFreshwater fishFish - MorphologyPeixes - ReproduçãoFertilizaçãoPeixe de água docePeixes - MorfologiaThis paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo diameter and embryo volume comparing the treatments versus control group (water) (p<0.05), this results can help in future cryopreservation protocols. Although the temperature and oxygenation differed between the usual and experimental incubators (p<0.05), the results showed a high fertilization rate (79.6 ± 13.2%) for experimental incubators (p<0.05) which is sufficient for the maintenance of embryos in a cryoprotective environment and effectively allows experimentation for long periods with cryoprotectant substances. Cryopreservation of fish embryos has not been accomplished yet and new approaches are required for understanding the permeability of teleost embryos, especially in Brazilian native species.Este trabalho avaliou o efeito de substâncias crioprotetoras sobre embriões de Prochilodus lineatus em uma incubadora experimental. O estudo aplicou combinações de álcool polivinílico, hidroxietilcelulose, gelatina e soro fetal bovino com dimetilosulfóxido e etilenoglicol em uma nova incubadora experimental. Foram avaliadas a morfologia dos embriões, a viabilidade larval e a eficiência das incubadoras experimentais na manutenção da qualidade dos embriões. Este estudo demonstra a associação eficiente entre hidroxietilcelulose e dimetilsulfóxido pela maior viabilidade (p<0,05) encontrada para os embriões (72,9 ± 23,9%). Deve-se notar também a permeação dos crioprotetores nos embriões através das alterações encontradas no diâmetro córion, diâmetro do embrião e no volume do embrião comparando os tratamentos ao grupo controle (água) (p<0,05), estes resultados podem ajudar em futuros protocolos de criopreservação. Embora a temperatura e a oxigenação diferiram entre as incubadoras comuns e as experimentais (p<0,05), os resultados mostraram elevada taxa de fertilização (79,6 ± 13,2%) para incubadoras experimentais (p<0,05), o que é suficiente para a manutenção de embriões em ambiente crioprotetor e permite efetivamente a experimentação por longos períodos com substâncias crioprotetoras. A criopreservação de embriões de peixes ainda não foi realizada e novas abordagens são necessárias para a compreensão da permeabilidade dos embriões de teleósteos, especialmente em espécies nativas brasileiras.Sociedade Brasileira de Ictiologia2020-03-06T17:00:14Z2020-03-06T17:00:14Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPAULA, D. A. J. et al. Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator. Neotropical Ichthyology, Porto Alegre, v. 12, n. 4, p. 835-844, out./dez. 2014.http://repositorio.ufla.br/jspui/handle/1/39221Neotropical Ichthyologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessPaula, Daniella Aparecida de JesusMachado, Mônica Rodrigues FerreiraMurgas, Luis David SolisRabelo, DenilsonZangerônimo, Márcio GilbertoReis Neto, Rafael VilhenaAndrade, Estefânia de SouzaAndrade, Eliane Aparecida deeng2020-03-06T17:00:34Zoai:localhost:1/39221Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-03-06T17:00:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
title Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
spellingShingle Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
Paula, Daniella Aparecida de Jesus
Fish - Reproduction
Fertilization
Freshwater fish
Fish - Morphology
Peixes - Reprodução
Fertilização
Peixe de água doce
Peixes - Morfologia
title_short Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
title_full Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
title_fullStr Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
title_full_unstemmed Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
title_sort Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator
author Paula, Daniella Aparecida de Jesus
author_facet Paula, Daniella Aparecida de Jesus
Machado, Mônica Rodrigues Ferreira
Murgas, Luis David Solis
Rabelo, Denilson
Zangerônimo, Márcio Gilberto
Reis Neto, Rafael Vilhena
Andrade, Estefânia de Souza
Andrade, Eliane Aparecida de
author_role author
author2 Machado, Mônica Rodrigues Ferreira
Murgas, Luis David Solis
Rabelo, Denilson
Zangerônimo, Márcio Gilberto
Reis Neto, Rafael Vilhena
Andrade, Estefânia de Souza
Andrade, Eliane Aparecida de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paula, Daniella Aparecida de Jesus
Machado, Mônica Rodrigues Ferreira
Murgas, Luis David Solis
Rabelo, Denilson
Zangerônimo, Márcio Gilberto
Reis Neto, Rafael Vilhena
Andrade, Estefânia de Souza
Andrade, Eliane Aparecida de
dc.subject.por.fl_str_mv Fish - Reproduction
Fertilization
Freshwater fish
Fish - Morphology
Peixes - Reprodução
Fertilização
Peixe de água doce
Peixes - Morfologia
topic Fish - Reproduction
Fertilization
Freshwater fish
Fish - Morphology
Peixes - Reprodução
Fertilização
Peixe de água doce
Peixes - Morfologia
description This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo diameter and embryo volume comparing the treatments versus control group (water) (p<0.05), this results can help in future cryopreservation protocols. Although the temperature and oxygenation differed between the usual and experimental incubators (p<0.05), the results showed a high fertilization rate (79.6 ± 13.2%) for experimental incubators (p<0.05) which is sufficient for the maintenance of embryos in a cryoprotective environment and effectively allows experimentation for long periods with cryoprotectant substances. Cryopreservation of fish embryos has not been accomplished yet and new approaches are required for understanding the permeability of teleost embryos, especially in Brazilian native species.
publishDate 2014
dc.date.none.fl_str_mv 2014
2020-03-06T17:00:14Z
2020-03-06T17:00:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PAULA, D. A. J. et al. Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator. Neotropical Ichthyology, Porto Alegre, v. 12, n. 4, p. 835-844, out./dez. 2014.
http://repositorio.ufla.br/jspui/handle/1/39221
identifier_str_mv PAULA, D. A. J. et al. Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator. Neotropical Ichthyology, Porto Alegre, v. 12, n. 4, p. 835-844, out./dez. 2014.
url http://repositorio.ufla.br/jspui/handle/1/39221
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
dc.source.none.fl_str_mv Neotropical Ichthyology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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