Haploid identification using tropicalized haploid inducer progenies in maize

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Camila Bastos
Data de Publicação: 2018
Outros Autores: Pereira, Francielly de Cássia, Nóbrega Filho, Lamartine da, Rezende, Breno Alvarenga, Dias, Kaio Olímpio das Graças, Braz, Guilherme Tomaz, Ruy, Murilo Candido, Silva, Maria Beatriz, Cenzi, Gisele, Techio, Vânia Helena, Souza, João Cândido de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/33241
Resumo: The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.
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spelling Haploid identification using tropicalized haploid inducer progenies in maizeFlow cytometryZea maysChromosome countingChromosome duplicationPloidy levelThe aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.Universidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT)2019-03-22T13:23:54Z2019-03-22T13:23:54Z2018-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, C. B. et al. Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 1, p. 16-23, Jan./Mar. 2018. DOI: 10.1590/1984-70332018v18n1a3.http://repositorio.ufla.br/jspui/handle/1/33241Crop Breeding and Applied Biotechnology (CBAB)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessRibeiro, Camila BastosPereira, Francielly de CássiaNóbrega Filho, Lamartine daRezende, Breno AlvarengaDias, Kaio Olímpio das GraçasBraz, Guilherme TomazRuy, Murilo CandidoSilva, Maria BeatrizCenzi, GiseleTechio, Vânia HelenaSouza, João Cândido deeng2019-03-22T13:23:54Zoai:localhost:1/33241Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-03-22T13:23:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Haploid identification using tropicalized haploid inducer progenies in maize
title Haploid identification using tropicalized haploid inducer progenies in maize
spellingShingle Haploid identification using tropicalized haploid inducer progenies in maize
Ribeiro, Camila Bastos
Flow cytometry
Zea mays
Chromosome counting
Chromosome duplication
Ploidy level
title_short Haploid identification using tropicalized haploid inducer progenies in maize
title_full Haploid identification using tropicalized haploid inducer progenies in maize
title_fullStr Haploid identification using tropicalized haploid inducer progenies in maize
title_full_unstemmed Haploid identification using tropicalized haploid inducer progenies in maize
title_sort Haploid identification using tropicalized haploid inducer progenies in maize
author Ribeiro, Camila Bastos
author_facet Ribeiro, Camila Bastos
Pereira, Francielly de Cássia
Nóbrega Filho, Lamartine da
Rezende, Breno Alvarenga
Dias, Kaio Olímpio das Graças
Braz, Guilherme Tomaz
Ruy, Murilo Candido
Silva, Maria Beatriz
Cenzi, Gisele
Techio, Vânia Helena
Souza, João Cândido de
author_role author
author2 Pereira, Francielly de Cássia
Nóbrega Filho, Lamartine da
Rezende, Breno Alvarenga
Dias, Kaio Olímpio das Graças
Braz, Guilherme Tomaz
Ruy, Murilo Candido
Silva, Maria Beatriz
Cenzi, Gisele
Techio, Vânia Helena
Souza, João Cândido de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Camila Bastos
Pereira, Francielly de Cássia
Nóbrega Filho, Lamartine da
Rezende, Breno Alvarenga
Dias, Kaio Olímpio das Graças
Braz, Guilherme Tomaz
Ruy, Murilo Candido
Silva, Maria Beatriz
Cenzi, Gisele
Techio, Vânia Helena
Souza, João Cândido de
dc.subject.por.fl_str_mv Flow cytometry
Zea mays
Chromosome counting
Chromosome duplication
Ploidy level
topic Flow cytometry
Zea mays
Chromosome counting
Chromosome duplication
Ploidy level
description The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
2019-03-22T13:23:54Z
2019-03-22T13:23:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, C. B. et al. Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 1, p. 16-23, Jan./Mar. 2018. DOI: 10.1590/1984-70332018v18n1a3.
http://repositorio.ufla.br/jspui/handle/1/33241
identifier_str_mv RIBEIRO, C. B. et al. Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 1, p. 16-23, Jan./Mar. 2018. DOI: 10.1590/1984-70332018v18n1a3.
url http://repositorio.ufla.br/jspui/handle/1/33241
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT)
publisher.none.fl_str_mv Universidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT)
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology (CBAB)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1784550106468450304

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